Light, Scanning Electron Microscopy and SDS-PAGE Studies on the Effect of the Essential Oil, Citrus sinensis Var. balady on the Embryonic Development of Camel Tick Hyalomma dromedarii (Koch, 1818) (Acari: Ixodidae)

2007 ◽  
Vol 10 (8) ◽  
pp. 1151-1160 ◽  
Author(s):  
Salwa M. Habeeb ◽  
Sobhy Abdel-Shaf ◽  
Abd El-Ghany A. Youssef
1986 ◽  
Vol 64 (9) ◽  
pp. 1994-1997 ◽  
Author(s):  
M. El Gohary ◽  
M. Y. Kamel ◽  
M. H. Madbouly

External egg morphology of the camel tick Hyalomma dromedarii was studied. Injection of egg-laying females with 1% trypan blue resulted in incorporation of vital stain in the oviposited eggs. Daily measurements of fresh eggs revealed changes in egg length and width during embryonic development. Scanning electron microscopy showed the outermost layer of the egg to be spongy and porous containing clear pits of different sizes. An elevated longitudinal hatching ridge encircled the egg chorion. The development of cephalothorax, appendages, Malpighian tubules, and rectal sacs were monitered.


2012 ◽  
Vol 554-556 ◽  
pp. 990-993
Author(s):  
Jian Zou ◽  
Hai Yan Gao ◽  
Jie Zeng

Scanning electron microscopy of samples showed that corn flour granules by fermented and wet-milling were angular, pentagonal or elliptical similar to native starches while samples with extruding and cooking were irregularly fibrous and large granules. Protein of samples with wet-milling exhibited an obvious DSC endotherm, To 85.81°C, Tp 92.95°C, Tc 101.73°C and ΔH 1.614J/g.While the fermented and extruded samples had no endotherm. Ultra-violet absorption spectrometry showed that absorbance of fermented and extruded samples increased and the λmax were slightly red shift. The λmax were 294nm, 297nm and 310nm for wet-milling , fermented and extruded flour, respectively. SDS-PAGE patterns of wet-milling flour contained four bands, fermented samples only three bands while extruded samples had same bands with dry-milling.


Zygote ◽  
2016 ◽  
Vol 25 (1) ◽  
pp. 17-31 ◽  
Author(s):  
Camila Marques ◽  
Francine Faustino ◽  
Bruno Bertolucci ◽  
Maria do Carmo Faria Paes ◽  
Regiane Cristina da Silva ◽  
...  

SummaryThe aim of this study was to characterize the embryonic development of Zungaro jahu, a fresh water teleostei commonly known as ‘jaú’. Samples were collected at pre-determined times from oocyte release to larval hatching and analysed under light microscopy, transmission electron microscopy and scanning electron microscopy. At the first collection times, the oocytes and eggs were spherical and yellowish, with an evident micropyle. Embryo development took place at 29.4 ± 1.5°C and was divided into seven stages: zygote, cleavage, morula, blastula, gastrula, organogenesis, and hatching. The differentiation of the animal and vegetative poles occured during the zygote stage, at 10 min post-fertilization (mpf), leading to the development of the egg cell at 15 mpf. From 20 to 75 mpf, successive cleavages resulted in the formation of 2, 4, 8, 16, 32 and 64 blastomeres. The morula stage was observed between 90 and 105 mpf, and the blastula and gastrula stage at 120 and 180 mpf; respectively. The end of the gastrula stage was characterized by the presence of the yolk plug at 360 mpf. Organogenesis followed, with differentiation of the cephalic and caudal regions, elongation of the embryo by the cephalo-caudal axis, and somitogenesis. Hatching occurred at 780 mpf, with mean larval total length of 3.79 ± 0.11 mm.


2020 ◽  
Vol 861 ◽  
pp. 378-382
Author(s):  
Sophoan Phal ◽  
Muhammad Rafiullah Khan ◽  
Pattarin Leelaphiwat ◽  
Vanee Chonhenchob

Sodium alginate based films containing turmeric oil (TEO) at different concentrations (1, 2 and 3%) were developed. The film with no TEO was used as control. Incorporation of TEO had the effects on the film properties. With increasing TEO concentrations, thickness, elongation at break, permeability of oxygen and water vapor of the films significantly (p ≤ 0.05) increased. Whereas moisture content, tensile strength and modulus of elasticity significantly (p ≤ 0.05) decreased. Scanning electron microscopy (SEM) analysis showed more numerous pores and rougher surface of the antifungal films than the control film.


2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.


2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Peyman Rajaei ◽  
Taher Nejadsattari ◽  
Ali Asghar Maassoumi ◽  
Valiollah Mozaffarian ◽  
Ali Sonboli

The essential oil was obtained from the aerial flowering parts of Tanacetum fisherae, a new record from Iran, by hydrodistillation and analyzed by GC and GC-MS. Eleven components representing 99.9% of the total oil were identified. 1,8-Cineole (79.9%) was characterized as the principal compound. The essential oil and its main component were tested against nine microbial strains. The result of the bioassays revealed that the oil possess potent antimicrobial property. This may be associated due to the presence of 1,8-cineole in the oil which tested individually and its high activity was observed. Micromorphological studies of hairs by scanning electron microscopy (SEM) exhibited the presence of abundant sessile capitate glandular and medifixed T-shaped eglandular trichomes on the leaves, flowers and achene, giving useful diagnostic characters for identification of this medicinal plant.


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