On the morphology of developing eggs of the camel tick Hyalomma dromedarii Koch, 1844

1986 ◽  
Vol 64 (9) ◽  
pp. 1994-1997 ◽  
Author(s):  
M. El Gohary ◽  
M. Y. Kamel ◽  
M. H. Madbouly
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Trypan Blue ◽  
Egg Laying ◽  
Malpighian Tubules ◽  
Hyalomma Dromedarii ◽  
Outermost Layer ◽  
Camel Tick ◽  
Egg Morphology ◽  
Scanning Electron

External egg morphology of the camel tick Hyalomma dromedarii was studied. Injection of egg-laying females with 1% trypan blue resulted in incorporation of vital stain in the oviposited eggs. Daily measurements of fresh eggs revealed changes in egg length and width during embryonic development. Scanning electron microscopy showed the outermost layer of the egg to be spongy and porous containing clear pits of different sizes. An elevated longitudinal hatching ridge encircled the egg chorion. The development of cephalothorax, appendages, Malpighian tubules, and rectal sacs were monitered.

Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)

2019 ◽  
Vol 57 (3) ◽  
pp. 745-754
Author(s):  
Francisco Augusto da Silva Ferreira ◽  
Rejane de Castro Simões ◽  
Ruth Leila Ferreira-Keppler ◽  
Jeronimo Alencar ◽  
Vera Margarete Scarpassa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Principal Component ◽  
Accuracy Rate ◽  
Morphometric Characters ◽  
Geometric Morphometry ◽  
Neotropical Species ◽  
Egg Morphology ◽  
Scanning Electron

Abstract The eggs of three Amazonian species of the genus Mansonia (Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralis Dyar & Knab, 1916 were morphologically different from those of Mansonia titillans (Walker, 1848) and Mansonia amazonensis (Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensis and Ma. titillans are more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.

A comparative SEM study of the eggs of the Sepsidae (Diptera) with a cladistic analysis based on egg, larval and adult characters

1995 ◽  
Vol 26 (4) ◽  
pp. 425-438 ◽  
Author(s):  
Rudolf Meier
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cladistic Analysis ◽  
Central Canal ◽  
Single Filament ◽  
Data Set ◽  
Sem Study ◽  
Egg Morphology ◽  
Existing Data ◽  
Scanning Electron

AbstractThe eggs of 21 species in 12 genera of Sepsidae were studied using scanning electron microscopy. All but the eggs of Ortalischema have respiratory filaments which aid in gas exchange. Judged by positional differences and the lack of a central canal, the two filaments of Orygma are not homologous to the single filament of most remaining sepsids. Hinton's hypothesis that egg filaments are plesiomorphic for the Sepsidae is therefore rejected. Three egg characters are added to an existing data set comprising 58 taxa and 85 larval and adult characters. The egg characters allow the unambiguous placement of Lasionemopoda, for which the larval and adult data set had suggested two different positions on equally parsimonious trees. Based on the position of Lasionemopoda, the evolution of the fore femora of the Sepsidae is briefly discussed. The egg morphology of the Sepsidae is also compared to the morphology of drosophiline eggs which also have egg filaments.

scholarly journals Comparison of Two Kinds of Cell Packets ofMicrococcus luteusby Scanning Electron Microscopy: Outermost Layer Maintaining the Packet Structure

1990 ◽  
Vol 34 (8) ◽  
pp. 691-696
Author(s):  
Toshihiko Monodane ◽  
Michiko Tokunaga ◽  
Hirotami Koike ◽  
Shozo Kotani ◽  
Michio Matsuhashi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Outermost Layer ◽  
Scanning Electron

Egg morphology update based on new chorionic data of Potamanthus luteus  (Linnaeus), Ephemera danica Müller and Oligoneuriella rhenana (Imhoff) (Insecta, Ephemeroptera) obtained by scanning electron microscopy

Zootaxa ◽  
2007 ◽  
Vol 1465 (1) ◽  
pp. 15-29 ◽  
Author(s):  
NICOLÁS UBERO-PASCAL ◽  
M. ANGELS PUIG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Adhesive Layer ◽  
Morphological Data ◽  
Current Trends ◽  
Sem Study ◽  
Egg Morphology ◽  
Ephemera Danica ◽  
Scanning Electron

The chorionic patterns of Ephemeroptera eggs are very diverse and these have often been used for taxonomic and systematic purposes. In a great number of species, including Potamanthus luteus, Ephemera danica and Oligoneuriella rhenana, these egg features have been studied using light microscopy. However, current trends in egg morphology studies use scanning electron microscopy (SEM), so that the eggs of these species need to be re-described in order to establish morphological comparisons. The general chorionic features which have already been described in these three species are confirmed in our SEM study, although a more detailed description of both the architecture and arrangement of these can now be offered. In addition, this study has allowed us to note new morphological data, such as the chorionic reticulation in P. luteus and the complex extrachorion-adhesive layer in E. danica; classification of the lateral attachment structure in P. luteus and O. rhenana has been changed; and the variability of the polar cap observed in P. luteus underlines the care that must to be taken when selecting chorionic structures for taxonomic purposes.

Scanning electron microscopy of eggs of Ochlerotatus taeniorhynchus (Diptera: Culicidae, Aedini)

Zootaxa ◽  
2012 ◽  
Vol 3509 (1) ◽  
pp. 77 ◽  
Author(s):  
CARLOS BRISOLA MARCONDES ◽  
GERSON AZULIM MÜLLER ◽  
JACENIR SANTOS-MALLET ◽  
JERONIMO ALENCAR ◽  
JULIANA SOARES SARMENTO ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
United States ◽  
Scanning Electron Microscopy ◽  
North Carolina ◽  
External Morphology ◽  
Aquatic Habitats ◽  
Coastal Habitats ◽  
Egg Morphology ◽  
Scanning Electron

The study of egg morphology is useful for the identification of material from aquatic habitats. For example, based on brief descriptions of external morphology of the eggs, Scotton and Axtell (1979) analysed the distribution of Ochlerotatus sollicitans (Walker) and Oc. taeniorhynchus (Wiedemann) in several coastal habitats in North Carolina, United States.

scholarly journals Egg shell morphology of an amblyceran louse, Hohorstiella rampurensis (Phthiraptera) infesting ring dove, Streptopelia decaocta

2016 ◽  
Vol 8 (1) ◽  
pp. 469-472
Author(s):  
Padam Singh ◽  
Jagirti Madan ◽  
Nidhi Gupta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Shell Morphology ◽  
Rice Grain ◽  
Ring Dove ◽  
Sem Study ◽  
Egg Morphology ◽  
Egg Shell ◽  
Scanning Electron

Scanning electron microscopy (SEM) study showed that miniature rice grain like egg shell of Hohorstiella rampurensis was covered by obliquely placed opercular disc. The apophyses were present only on one side of the egg shell. There were 25-35 long elongated rod like apophyses (apically turned), which were arranged in 3-4 rows and occurred in pair. The nature of egg shell of H. rampurensis characteristically differed from that of H. lata (the only species of Hohorstiella studied, so far). The study further indicates about the role of egg morphology as a guide to louse taxonomy.

Preparation of Amyl Acetate and Acetone Labile Eggs From Parasitic Nematodes For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 440-441
Author(s):  
Robert D. Specian ◽  
Venita F. Allison ◽  
J. E. Ubelaker ◽  
J. H. Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Toxocara Canis ◽  
Parasitic Nematodes ◽  
Lipid Complex ◽  
Critical Point Drying ◽  
Egg Morphology ◽  
Nematode Eggs ◽  
Egg Shell ◽  
Scanning Electron

The structure and composition of parasitic nematode eggs, particularly the shells, are of interest to various investigators. Egg morphology is widely used for species identification where sacrifice of the host is not desirable. Scanning electron microscopy (SEM) allows surface sculpture patterns on eggs of closely related species to be observed and aids in identification. The egg shell, in many species of parasitic nematodes, is composed of a proteinchitin-lipid complex which, unfortunately, is labile in many organic solvents including those routinely employed in the preparation of samples for SEM by the critical point drying method.The present study is concerned with the fine surface morphology of eggs from Toxocara canis (Werner, 1782), Toxocara mystax (Zeder, 1800), and Ascaris suum Goeze, 1782, as revealed by SEM after several preparative procedures.

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