Ultrastructural Changes in the Myelinated Nerve Fibers of the Sciatic Nerve in Galactose Intoxication in Rats

2012 ◽  
Vol 11 (4) ◽  
pp. 391-400 ◽  
Author(s):  
Faris M. Altaf
Keyword(s):  
Sciatic Nerve ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers

scholarly journals Combination of Acellular Nerve Graft and Schwann Cells-Like Cells for Rat Sciatic Nerve Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Songtao Gao ◽  
Yan Zheng ◽  
Qiqing Cai ◽  
Zhansheng Deng ◽  
Weitao Yao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sciatic Nerve ◽  
Gastrocnemius Muscle ◽  
Nerve Fibers ◽  
Recovery Ratio ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Nerve Grafts ◽  
Group A ◽  
Wet Weight

Objective.To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.Methods.Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.Results.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P<0.05), and the difference between groups B and C was not statistically significant (P>0.05).Conclusion.The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

Regeneration of myelinated nerve fibers into branches of the sciatic nerve rapaired with treated muscle grafts

1990 ◽  
Vol 3 (2) ◽  
pp. 121-132 ◽  
Author(s):  
C. L. -H. Huang ◽  
J. M. Gattuso ◽  
M. A. Glasby ◽  
S. E. Gschmeissner
Keyword(s):  
Sciatic Nerve ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers

Morphometric analysis of the diameter and g-ratio of the myelinated nerve fibers of the human sciatic nerve during the aging process

2015 ◽  
Vol 91 (3) ◽  
pp. 238-245 ◽  
Author(s):  
Sladjana Ugrenović ◽  
Ivan Jovanović ◽  
Ljiljana Vasović ◽  
Braca Kundalić ◽  
Rade Čukuranović ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Morphometric Analysis ◽  
Aging Process ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
G Ratio

scholarly journals THE ULTRASTRUCTURAL LOCALIZATION OF CHOLINESTERASE ACTIVITY IN THE SCIATIC NERVE OF THE RAT

1966 ◽  
Vol 14 (5) ◽  
pp. 369-378 ◽  
Author(s):  
WILLIAM W. SCHLAEPFER ◽  
RICHARD M. TORACK
Keyword(s):  
Sciatic Nerve ◽  
Enzymatic Activity ◽  
Cholinesterase Activity ◽  
Ultrastructural Localization ◽  
Nerve Fibers ◽  
Specific Inhibition ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Rat Sciatic Nerve ◽  
Substrate Preferences

Ultrastructural localization of cholinesterase activity was demonstrated in rat sciatic nerve using thiocholine esters as substrate. This enzymatic activity was limited to axons, where it was located on limiting axonal membranes, and on axonal vesicles of all unmyelinated and some myelinated nerve fibers. No accentuation of activity was present at nodes of Ranvier. The use of acetyl, propionyl and butyrylthiocholine as substrate resulted in decreasing amounts of end product precipitate, respectively. The cholinesterase activity was sensitive to heat, resistant to N-ethyl maleimide, and inhibited byeserine, DFP, E600, RO2-1250 and BW 62C47. Studies of specific inhibition sensitivities and substrate preferences suggested that the axonal cholinesterase of rat sciatic nerve is a specific acetylcholinesterase which is relatively homogeneous.

HISTOMETRIC AND ULTRASTRUCTURAL ORGANIZATION OF THE NERVIMUSCULAR TERMINALS OF SKELETAL MUSCLES AT A HYPOKINESIA

2019 ◽  
pp. 55-63
Author(s):  
Z. M. Yaschyshyn ◽  
S. L. Popel
Keyword(s):  
Skeletal Muscles ◽  
Structural Adjustment ◽  
Muscle Fibers ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Myelinated Nerve ◽  
Male Wistar Rats

The aim: to study the dynamics of histological and ultrastructural changes in muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia at different stages of ontogenesis. Methods. Studied skeletal muscles and their peripheral nervous apparatus of laboratory male Wistar rats aged 30 to 270 days. The restriction of motor activity was carried out in special canister cells for 30, 60, 90, and 240 days (5 animals for each term). To determine the type of muscle fiber, the Nahlas histochemical method was used, the Kulchitsky method was used to detect myelinated nerve fibers, the Bilshovsky-Gros method and the electron microscopic method to identify neuromuscular endings. Results. The data of histological and electron microscopic examination of skeletal muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia indicate their regular restructuring during the development of muscles, the formation of their synapses and structures that are associated with them at different stages of ontogenesis. Conclusion. The study provides an in-depth understanding of the relative frequency and nature of the disturbance of the neuromuscular endings during prolonged hypokinesia and its effect on the dynamics of structural adjustment of individual types of muscle fibers in ontogenesis.

HISTOMETRIC AND ULTRASTRUCTURAL ORGANIZATION OF THE NERVIMUSCULAR TERMINALS OF SKELETAL MUSCLES AT A HYPOKINESIA

2019 ◽  
pp. 55-63
Author(s):  
Z. M. Yaschyshyn ◽  
S. L. Popel
Keyword(s):  
Skeletal Muscles ◽  
Structural Adjustment ◽  
Muscle Fibers ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Myelinated Nerve ◽  
Male Wistar Rats

The aim: to study the dynamics of histological and ultrastructural changes in muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia at different stages of ontogenesis. Methods. Studied skeletal muscles and their peripheral nervous apparatus of laboratory male Wistar rats aged 30 to 270 days. The restriction of motor activity was carried out in special canister cells for 30, 60, 90, and 240 days (5 animals for each term). To determine the type of muscle fiber, the Nahlas histochemical method was used, the Kulchitsky method was used to detect myelinated nerve fibers, the Bilshovsky-Gros method and the electron microscopic method to identify neuromuscular endings. Results. The data of histological and electron microscopic examination of skeletal muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia indicate their regular restructuring during the development of muscles, the formation of their synapses and structures that are associated with them at different stages of ontogenesis. Conclusion. The study provides an in-depth understanding of the relative frequency and nature of the disturbance of the neuromuscular endings during prolonged hypokinesia and its effect on the dynamics of structural adjustment of individual types of muscle fibers in ontogenesis.

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