scholarly journals THE ULTRASTRUCTURAL LOCALIZATION OF CHOLINESTERASE ACTIVITY IN THE SCIATIC NERVE OF THE RAT

1966 ◽  
Vol 14 (5) ◽  
pp. 369-378 ◽  
Author(s):  
WILLIAM W. SCHLAEPFER ◽  
RICHARD M. TORACK
Keyword(s):  
Sciatic Nerve ◽  
Enzymatic Activity ◽  
Cholinesterase Activity ◽  
Ultrastructural Localization ◽  
Nerve Fibers ◽  
Specific Inhibition ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Rat Sciatic Nerve ◽  
Substrate Preferences

Ultrastructural localization of cholinesterase activity was demonstrated in rat sciatic nerve using thiocholine esters as substrate. This enzymatic activity was limited to axons, where it was located on limiting axonal membranes, and on axonal vesicles of all unmyelinated and some myelinated nerve fibers. No accentuation of activity was present at nodes of Ranvier. The use of acetyl, propionyl and butyrylthiocholine as substrate resulted in decreasing amounts of end product precipitate, respectively. The cholinesterase activity was sensitive to heat, resistant to N-ethyl maleimide, and inhibited byeserine, DFP, E600, RO2-1250 and BW 62C47. Studies of specific inhibition sensitivities and substrate preferences suggested that the axonal cholinesterase of rat sciatic nerve is a specific acetylcholinesterase which is relatively homogeneous.

scholarly journals Combination of Acellular Nerve Graft and Schwann Cells-Like Cells for Rat Sciatic Nerve Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Songtao Gao ◽  
Yan Zheng ◽  
Qiqing Cai ◽  
Zhansheng Deng ◽  
Weitao Yao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sciatic Nerve ◽  
Gastrocnemius Muscle ◽  
Nerve Fibers ◽  
Recovery Ratio ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Nerve Grafts ◽  
Group A ◽  
Wet Weight

Objective.To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.Methods.Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.Results.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P<0.05), and the difference between groups B and C was not statistically significant (P>0.05).Conclusion.The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

scholarly journals CHOLINESTERASE ACTIVITY OF NODAL AND INTERNODAL REGIONS OF MYELINATED NERVE FIBERS OF FROG

1967 ◽  
Vol 32 (3) ◽  
pp. 577-583 ◽  
Author(s):  
Miro Brzin ◽  
Wolf-D. Dettbarn
Keyword(s):  
Enzyme Activity ◽  
Esterase Activity ◽  
Cholinesterase Activity ◽  
Node Of Ranvier ◽  
Nerve Fibers ◽  
Selective Inhibitors ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Significant Difference ◽  
Saltatory Conduction

The distribution of cholinesterase (Ch-esterase) in isolated myelinated fibers of the frog has been investigated. Quantitative microgasometric measurements have confirmed the previous histochemical observations. Both approaches indicate that in frog nerve fibers acetylcholinesterase (ACh-esterase) is the only or the predominant enzyme when selective inhibitors and different substrates are used: acetylcholine (ACh), butyrylcholine, and acetyl-B-methylcholine (Mecholyl). By means of the microgasometric technique, a significant difference in ACh-esterase activity between axons isolated from ventral (37.2 ± 1.7 µmole x 10-5 ACh/mm2/hr) and dorsal roots (2.0 ± 0.9 µmole x 10-5 ACh/mm2/hr) was found. In the region of the node of Ranvier the enzyme activity (50.4 ± 4.4 µmole x 10-5 ACh/mm2/hr) appears to be considerably higher than in the internodal area (36.6 ± 2.1 µmole x 10-5 ACh/mm2/hr). The findings are discussed in relation to the theory of saltatory conduction and the ACh system.

Regeneration of myelinated nerve fibers into branches of the sciatic nerve rapaired with treated muscle grafts

1990 ◽  
Vol 3 (2) ◽  
pp. 121-132 ◽  
Author(s):  
C. L. -H. Huang ◽  
J. M. Gattuso ◽  
M. A. Glasby ◽  
S. E. Gschmeissner
Keyword(s):  
Sciatic Nerve ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers

Morphometric analysis of the diameter and g-ratio of the myelinated nerve fibers of the human sciatic nerve during the aging process

2015 ◽  
Vol 91 (3) ◽  
pp. 238-245 ◽  
Author(s):  
Sladjana Ugrenović ◽  
Ivan Jovanović ◽  
Ljiljana Vasović ◽  
Braca Kundalić ◽  
Rade Čukuranović ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Morphometric Analysis ◽  
Aging Process ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
G Ratio
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