scholarly journals A Role of YlBud8 in the Regulation of Cell Separation in the Yeast Yarrowia lipolytica

2019 ◽  
Vol 29 (1) ◽  
pp. 141-150
Author(s):  
Li Yun Qing ◽  
Xue Qing Jie ◽  
Yang Yuan Yuan ◽  
Wang Hui ◽  
Li Xiu Zhen
Keyword(s):  
Yarrowia Lipolytica ◽  
Cell Separation ◽  
Yeast Yarrowia Lipolytica

scholarly journals The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast Yarrowia lipolytica

2021 ◽  
Vol 22 (17) ◽  
pp. 9282
Author(s):  
Piotr Hapeta ◽  
Patrycja Szczepańska ◽  
Tadeusz Witkowski ◽  
Jean-Marc Nicaud ◽  
Anne-Marie Crutz-Le Coq ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Sugar Industry ◽  
Pentose Phosphate ◽  
Glucose Depletion ◽  
Hexose Transporters ◽  
Growth Profiles ◽  
Kinetics Of ◽  
Sucrose Utilization ◽  
Yeast Yarrowia Lipolytica

The development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product of the sugar industry, contains mostly sucrose, a disaccharide composed of glucose and fructose, both easily absorbed by microorganisms. Yarrowia lipolytica, a platform for the production of various chemicals, can be engineered for sucrose utilization by heterologous invertase expression, yet the problem of preferential use of glucose over fructose remains, as fructose consumption begins only after glucose depletion what significantly extends the bioprocesses. We investigated the role of hexose transporters and hexokinase (native and fructophilic) in this preference. Analysis of growth profiles and kinetics of monosaccharide utilization has proven that the glucose preference in Y. lipolytica depends primarily on the affinity of native hexokinase for glucose. Interestingly, combined overexpression of either hexokinase with hexose transporters significantly accelerated citric acid biosynthesis and enhanced pentose phosphate pathway leading to secretion of polyols (31.5 g/L vs. no polyols in the control strain). So far, polyol biosynthesis was efficient in glycerol-containing media. Moreover, overexpression of fructophilic hexokinase in combination with hexose transporters not only shortened this process to 48 h (84 h for the medium with glycerol) but also allowed to obtain 23% more polyols (40 g/L) compared to the glycerol medium (32.5 g/L).

scholarly journals The Overexpression of YALI0B07117g Results in EnhancedErythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7549
Author(s):  
Mateusz Szczepańczyk ◽  
Dorota A. Rzechonek ◽  
Adam Dobrowolski ◽  
Aleksandra M. Mirończuk
Keyword(s):  
Carbon Source ◽  
Yarrowia Lipolytica ◽  
Sole Carbon Source ◽  
Consumption Rate ◽  
Batch Cultures ◽  
Cultivation Time ◽  
Erythrose Reductase ◽  
Yeast Yarrowia Lipolytica ◽  
Rate Of Consumption

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog YALI0B07117g in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began. The strain overexpressing the YALI0B07117g gene was used to increase the erythritol yield from glycerol as a sole carbon source in batch cultures, resulting in a yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the modified strain and the productivity of erythritol increased from 0.28 g/(L h) for the A101 strain to 0.41 g/(L h ) for the modified strain. The application of the research may prove positive for shortening the cultivation time due to the increased rate of consumption of the substrate combined with the increased parameters of erythritol synthesis.

Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109

2014 ◽  
Vol 99 (4) ◽  
pp. 1637-1645 ◽  
Author(s):  
Guang-Yuan Wang ◽  
Yan Zhang ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Zhi-Peng Wang ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Pyruvate Carboxylase ◽  
Oleaginous Yeast ◽  
Intracellular Lipid ◽  
Yeast Yarrowia Lipolytica

scholarly journals Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 32 ◽  
Author(s):  
Mugdha Apte ◽  
Devashree Sambre ◽  
Shital Gaikawad ◽  
Swanand Joshi ◽  
Ashok Bankar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

scholarly journals Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sivamoke Dissook ◽  
Tomohisa Kuzuyama ◽  
Yuri Nishimoto ◽  
Shigeru Kitani ◽  
Sastia Putri ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Authentic Standard ◽  
Chemical Inhibition ◽  
Chromatographic Peaks ◽  
Methyl Erythritol Phosphate ◽  
Limiting Condition ◽  
Yeast Yarrowia Lipolytica

AbstractMethyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.

scholarly journals Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Thomas Beneyton ◽  
Stéphane Thomas ◽  
Andrew D. Griffiths ◽  
Jean-Marc Nicaud ◽  
Antoine Drevelle ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Throughput ◽  
High Throughput Screening ◽  
Yeast Yarrowia Lipolytica
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