Novel Method for ELISA Traces Pipetting System by Air Displacement Pipetting

2011 ◽  
Vol 103 ◽  
pp. 252-256 ◽  
Author(s):  
Lian Qing Zhu ◽  
Hong Li ◽  
Yun Xiao Na ◽  
Yang Kuan Guo ◽  
Ming Li Dong
Keyword(s):  
Real Time ◽  
Immunosorbent Assay ◽  
Pressure Sensor ◽  
Applied Pressure ◽  
Elisa Test ◽  
Liquid Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
The Real ◽  
Novel Method

In the Enzyme-linked immunosorbent assay (ELISA) test, many steps need traces pipetting. The ELISA test results will be different when we use different pipetting ways. Our traces pipetting system is based on the air displacement pipetting principle, comparable to the functioning of hand pipettes. It is applied pressure sensor to realize pressure-based liquid level detection (pLLD) and aspiration monitoring. The monitored system can distinguish the following situations: (1) a correct aspiration; (2) cup empty; (3) tip-blocked; (4) bubbles. Using the air displacement principle into traces pipetting can avoid contamination or dilution by system liquids, and problems with corroded tubing, pumps, etc. It applied pressure sensor to realize pLLD and aspiration monitoring. The results of the real-time monitor module on air displacement pipetting show that the traces pipetting system can agilely distinguish the different liquid pipetting situations. The method of air displacement pipetting offered an effective way for ELISA traces pipetting system.

scholarly journals Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW)

2019 ◽  
Vol 20 (1) ◽  
pp. 30
Author(s):  
Rinaldi Ghurafa ◽  
Denny Widaya Lukman ◽  
Hadri Latif
Keyword(s):  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Kappa Value ◽  
Alternative Test ◽  
The Rose ◽  
Higher Sensitivity ◽  
Good Agreement

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.

scholarly journals Novel Real-time Digital Pressure Sensor Reveals Wide Variations in Current Nerve Crush Injury Models

2021 ◽  
Vol 186 (Supplement_1) ◽  
pp. 473-478
Author(s):  
Grant D Wandling ◽  
Jung Il Lee ◽  
M A Hassan Talukder ◽  
Prem Kumar Govindappa ◽  
John C Elfar
Keyword(s):  
Real Time ◽  
Pressure Sensor ◽  
Coefficient Of Variation ◽  
Time Pressure ◽  
Applied Pressure ◽  
Crush Injury ◽  
Nerve Crush ◽  
The Real ◽  
Needle Driver ◽  
Nerve Crush Injury

ABSTRACT Introduction: Peripheral nerve crush injury (PNCI) models are commonly used to study nerve damage and the potential beneficial effects of novel therapeutic strategies. Current models of PNCI rely on inter-device and operator precision to limit the variation with applied pressure. Although the inability to accurately quantify the PNCI pressure may result in reduced reproducibility between animals and studies, there is very limited information on the standardization and quantification of applied pressure with PNCI. To address this deficit, we constructed a novel device comprised of an Arduino UNO microcontroller board and Force Sensitive Resistor capable of reporting the real-time pressure applied to a nerve. Methods: Two forceps and two needle drivers were used to perform 30-second PNCIs to the sciatic nerves of mice (n = 5/group). Needle drivers were set to the first notch, and a jig was used to hold the forceps pinch at a reproducible pressure. The Force Sensitive Resistor was interposed in-series between the nerve and instrument during PNCI. Results: Data collected from these procedures displayed average needle driver pressures an order of multitude greater than forceps pressures. Additionally, needle driver inter- and intra-procedure pressure remained more consistent than forceps pressure, with needle driver coefficient of variation equal to 14.5% vs. a forceps coefficient of variation equal to 45.4%. Conclusions: This is the first demonstration of real-time pressure measurements in PNCI models and it reveals that the applied pressures are dependent on the types of device used. The large disparity in pressure represents an inability to apply graded accurate and consistent intermediate pressure gradients in PNCI. These findings indicate a need for documentation of pressure severity as a screening for PNCI in animals, and the real-time pressure sensor could be a useful tool in monitoring and applying consistent pressure, reducing the outcome variability within the same experimental model of PNCI.

Studies on the enzyme linked immunosorbent assay (ELISA) test forSchistosoma mansoniinfections

1978 ◽  
Vol 72 (3) ◽  
pp. 243-253 ◽  
Author(s):  
M. McLaren ◽  
C. C. Draper ◽  
J. M. Roberts ◽  
E. Minter-Goedbloed ◽  
G. S. Ligthart ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay and Microbiologic Culture for Diagnosis of Staphylococcus aureus Intramammary Infection in Cows

1996 ◽  
Vol 59 (1) ◽  
pp. 6-10 ◽  
Author(s):  
PAUL C. BARTLETT ◽  
RONALD J. ERSKINE ◽  
PATRICK GASTON ◽  
PHILIP M. SEARS ◽  
HENDRICUS WILHELMUS HOUDIJK
Keyword(s):  
Staphylococcus Aureus ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Relative Sensitivity ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Status ◽  
Intramammary Infection ◽  
Previous Infection ◽  
Current Infection

Recent reports have indicated that the relative sensitivity and specificity of the ELISA test for detection of intramammary infection of cows with Staphylococcus aureus is not as high as originally reported. It has been suggested that antibodies measured by enzyme-linked immunosorbent assay (ELISA) more closely reflect previous infection status rather than current infection status, and that the delay in antibody formation following infection and the persistence of antibodies after elimination of infection may be responsible for some of the discrepancy observed between ELISA and bacterial culture results conducted on the same milk sample. This study (n = 209 cows) was undertaken to determine if an ELISA for S. aureus intramammary infection more closely reflects previous infection status than it does current infection status, and to ascertain whether correction of this time-delay factor substantially improves calculated values of ELISA relative sensitivity and specificity. Receiver-operator curves were constructed to compare different time-related definitions of microbiologic culture results used for comparison with ELISA results. A greater degree of curvature in receiver-operator curves indicated that ELISA results did more closely reflect culture results performed on milk samples taken 1 and 3 weeks previously. Insignificant improvement in sensitivity and specificity occurred when the database was limited to cows (n = 140) with milk production greater than 13.6 kg/day. However, values of sensitivity were all less than or equal to 90%, and values of specificity were all less than 54%.

scholarly journals Antibody Responses to CampylobacterInfections Determined by an Enzyme-Linked Immunosorbent Assay: 2-Year Follow-Up Study of 210 Patients

2001 ◽  
Vol 8 (2) ◽  
pp. 314-319 ◽  
Author(s):  
Mette Aagaard Strid ◽  
Jørgen Engberg ◽  
Lena Brandt Larsen ◽  
Kamilla Begtrup ◽  
Kåre Mølbak ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Large Degree ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Campylobacter Coli ◽  
Negative Results ◽  
Heat Stable Antigen

ABSTRACT An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody toCampylobacter jejuni and Campylobacter coli. Heat-stable antigen, a combination of C. jejuni serotype O:1,44 and O:53 in the ratio 1:1, was used as a coating antigen in the ELISA test. A total of 631 sera from 210 patients with verifiedCampylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With a 90th percentile of specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). At follow-up of the patients, IgG antibodies were elevated 4.5 months after infection but exhibited a large degree of variation in antibody decay profiles. IgA and IgM antibodies were elevated during the acute phase of infection (up to 2 months from onset of infection). The antibody response did not depend on Campylobacter species or C. jejuniserotype, with the important exception of response to C. jejuni O:19, the serotype most frequently associated with Guillain-Barré syndrome. All of the patients infected with this serotype had higher levels of both IgM (P = 0.006) and IgA (P = 0.06) compared with other C. jejuni and C. coli serotypes.

Preliminary Report about the Efficacy of Prototype Pressure Sensor for the Real-Time Intravesical Pressure Monitoring in the Rabbit

2012 ◽  
Vol 30 (1) ◽  
pp. 80 ◽  
Author(s):  
Su Jin Kim ◽  
Dong Sup Lee ◽  
Jong Chan Kim ◽  
Ho Young Lee ◽  
Bumkyoo Choi ◽  
...  
Keyword(s):  
Real Time ◽  
Pressure Sensor ◽  
Preliminary Report ◽  
Intravesical Pressure ◽  
Pressure Monitoring ◽  
The Real

Rhizobium strain identification in Arachis hypogaea nodules by enzyme-linked immunosorbent assay (ELISA)

1978 ◽  
Vol 24 (12) ◽  
pp. 1537-1543 ◽  
Author(s):  
B. Kishinevsky ◽  
M. Bar-Joseph
Keyword(s):  
Arachis Hypogaea ◽  
Immunosorbent Assay ◽  
Root Nodules ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Strain Identification ◽  
Single Root ◽  
Rhizobium Strains ◽  
Serological Identification ◽  
Phosphate Buffered Saline

The technique of enzyme-linked immunosorbent assay (ELISA) was used for serological identification of peanut Rhizobium strains both in cell suspension of pure culture and in single root nodules of groundnut (Arachis hypogaea) plants. Antisera of three peanut Rhizobium strains were tested against eight different Rhizobium isolates. Three serogroups identified by agglutination and immunodiffusion tests were confirmed by ELISA.In this experiment ELISA was more sensitive by four to six orders of magnitude than the agglutination and immunodiffusion tests and enabled the detection of Rhizobium antigens in cell suspensions of 104–105 cells per millilitre.The reactions of culture and nodule antigens were identical for all strains investigated.ELISA enabled the precise typing of rhizobial isolates in single small root nodules. The minimum fresh weight of nodule tissue necessary to perform the ELISA test was 0.4 mg crushed in 1 ml of phosphate-buffered saline (PBS).ELISA was also successfully used for strain identification in mixed inoculated plants. One of the strains in each pair formed most of the nodules examined.

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