scholarly journals Novel Real-time Digital Pressure Sensor Reveals Wide Variations in Current Nerve Crush Injury Models

2021 ◽  
Vol 186 (Supplement_1) ◽  
pp. 473-478
Author(s):  
Grant D Wandling ◽  
Jung Il Lee ◽  
M A Hassan Talukder ◽  
Prem Kumar Govindappa ◽  
John C Elfar

ABSTRACT Introduction: Peripheral nerve crush injury (PNCI) models are commonly used to study nerve damage and the potential beneficial effects of novel therapeutic strategies. Current models of PNCI rely on inter-device and operator precision to limit the variation with applied pressure. Although the inability to accurately quantify the PNCI pressure may result in reduced reproducibility between animals and studies, there is very limited information on the standardization and quantification of applied pressure with PNCI. To address this deficit, we constructed a novel device comprised of an Arduino UNO microcontroller board and Force Sensitive Resistor capable of reporting the real-time pressure applied to a nerve. Methods: Two forceps and two needle drivers were used to perform 30-second PNCIs to the sciatic nerves of mice (n = 5/group). Needle drivers were set to the first notch, and a jig was used to hold the forceps pinch at a reproducible pressure. The Force Sensitive Resistor was interposed in-series between the nerve and instrument during PNCI. Results: Data collected from these procedures displayed average needle driver pressures an order of multitude greater than forceps pressures. Additionally, needle driver inter- and intra-procedure pressure remained more consistent than forceps pressure, with needle driver coefficient of variation equal to 14.5% vs. a forceps coefficient of variation equal to 45.4%. Conclusions: This is the first demonstration of real-time pressure measurements in PNCI models and it reveals that the applied pressures are dependent on the types of device used. The large disparity in pressure represents an inability to apply graded accurate and consistent intermediate pressure gradients in PNCI. These findings indicate a need for documentation of pressure severity as a screening for PNCI in animals, and the real-time pressure sensor could be a useful tool in monitoring and applying consistent pressure, reducing the outcome variability within the same experimental model of PNCI.

2011 ◽  
Vol 103 ◽  
pp. 252-256 ◽  
Author(s):  
Lian Qing Zhu ◽  
Hong Li ◽  
Yun Xiao Na ◽  
Yang Kuan Guo ◽  
Ming Li Dong

In the Enzyme-linked immunosorbent assay (ELISA) test, many steps need traces pipetting. The ELISA test results will be different when we use different pipetting ways. Our traces pipetting system is based on the air displacement pipetting principle, comparable to the functioning of hand pipettes. It is applied pressure sensor to realize pressure-based liquid level detection (pLLD) and aspiration monitoring. The monitored system can distinguish the following situations: (1) a correct aspiration; (2) cup empty; (3) tip-blocked; (4) bubbles. Using the air displacement principle into traces pipetting can avoid contamination or dilution by system liquids, and problems with corroded tubing, pumps, etc. It applied pressure sensor to realize pLLD and aspiration monitoring. The results of the real-time monitor module on air displacement pipetting show that the traces pipetting system can agilely distinguish the different liquid pipetting situations. The method of air displacement pipetting offered an effective way for ELISA traces pipetting system.


2007 ◽  
Vol 205 (3) ◽  
pp. S92
Author(s):  
Terence M. Myckatyn ◽  
Christina Kenney ◽  
Alice Tong ◽  
Jessica Duan ◽  
Daniel Hunter ◽  
...  

1998 ◽  
Vol 96 (3-4) ◽  
pp. 245-254 ◽  
Author(s):  
Khalaf Al Moutaery ◽  
Mohammed Arshaduddin ◽  
Mohammad Tariq ◽  
Saleh Al Deeb

2017 ◽  
Vol 162 ◽  
pp. 97-103 ◽  
Author(s):  
Zhen Puyang ◽  
Hai-Qing Gong ◽  
Shi-Gang He ◽  
John B. Troy ◽  
Xiaorong Liu ◽  
...  

Neurosurgery ◽  
2021 ◽  
Vol 89 (Supplement_2) ◽  
pp. S139-S139
Author(s):  
Meei-Ling Sheu ◽  
Chiung-Chyi Shen ◽  
Hsi-Kai Tsou ◽  
Meng Yin Yang ◽  
Hong-Lin Su ◽  
...  

2018 ◽  
Vol 28 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Diego Noé Rodríguez Sánchez ◽  
Luiz Antonio de Lima Resende ◽  
Giovana Boff Araujo Pinto ◽  
Ana Lívia de Carvalho Bovolato ◽  
Fábio Sossai Possebon ◽  
...  

Crush injuries in peripheral nerves are frequent and induce long-term disability with motor and sensory deficits. Due to axonal and myelin sheath disruptions, strategies for optimized axonal regeneration are needed. Multipotent mesenchymal stromal cells (MSC) are promising because of their anti-inflammatory properties and secretion of neurotrophins. The present study investigated the effect of canine adipose tissue MSC (Ad-MSC) transplantation in an experimental sciatic nerve crush injury. Wistar rats were divided into three groups: sham ( n = 8); Crush+PBS ( n = 8); Crush+MSC ( n = 8). Measurements of sciatic nerve functional index (SFI), muscle mass, and electromyography (EMG) were performed. Canine Ad-MSC showed mesodermal characteristics (CD34-, CD45-, CD44+, CD90+ and CD105+) and multipotentiality due to chondrogenic, adipogenic, and osteogenic differentiation. SFI during weeks 3 and 4 was significantly higher in the Crush+MSC group ( p < 0.001). During week 4, the EMG latency in the Crush+MSC groups had better near normality ( p < 0.05). The EMG amplitude showed results close to normality during week 4 in the Crush+MSC group ( p < 0.04). There were no statistical differences in muscle weight between the groups ( p > 0.05), but there was a tendency toward weight gain in the Crush+MSC groups. Better motor functional recovery after crush and perineural canine Ad-MSC transplantation was observed during week 2. This was maintained till week 4. In conclusion, the canine Ad-MSC transplantation showed early pro-regenerative effects between 2–4 weeks in the rat model of sciatic nerve crush injury.


2018 ◽  
Vol 9 (1) ◽  
pp. 167-174
Author(s):  
Binbin Wang ◽  
Shiwei Wang ◽  
Song Liu ◽  
Shaodong Zhang ◽  
Dezhi Li ◽  
...  

Abstract Introduction This study investigated the effect of combining hypoglossal-facial nerve “side”-to-side neurorrhaphy and electrical myostimulation in a rat model of facial palsy. Methods Rats with facial nerve crush injury were subjected to control condition, monotherapy of either neurorrhaphy or electrical myostimulation, or bitherapy of the two treatments. After 1, 3, and 6 months, rats were performed the facial symmetry evaluation, electrophysiological examination and the retrograde labeling of motor neurons. Results As early as 3 months after injury, face symmetry significantly improved in rats of the bitherapy group. At 3 or 6 months after injury, either the parameters of electrophysiological examination or the number of labeled motor neurons were significantly increased in the bitherapy group than in any other group. Discussion The combination of neurorrhaphy and electrical myostimulation effectively promoted the functional recovery after facial nerve crush injury.


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