Optimization of DPPH Radical Scavenging Activity of Exopolysaccharides from Marine P. chrysogenum HGQ6 in Submerged Fermentation Using Response Surface Methodology

2011 ◽  
Vol 140 ◽  
pp. 379-384
Author(s):  
Gan Lin Guo ◽  
Lei Guo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Fermentation Medium ◽  
Fractional Factorial ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The objectives of this study were to investigate the effect of fermentation medium on the DPPH radical scavenging activity of exopolysaccharides from marine Penicillium chrysogenum HGQ6 by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Maltose, FeSO4, and K2HPO4 were important factors significantly affecting DPPH radical scavenging activity. These selected variables were subsequently optimized using a Box-Behnken design, and response surface analysis. The optimal medium compositions were (% w/v): maltose 2.71, FeSO4 0.0016, K2HPO4 0.1, and KNO3 1.0. Under these optimal conditions, the DPPH radical scavenging rate achieved 34.0%, which agreed with the predicted values.

Use of Response Surface Methodology for Phenolic Antioxidant Extraction from Lotus (Nelumbo nucifera Gaertn.) Pod Pericarp

2012 ◽  
Vol 506 ◽  
pp. 591-594 ◽  
Author(s):  
P. Pintathong ◽  
P. Chanphithak ◽  
P. Sereetaveekul ◽  
N. Thitipramote ◽  
P. Chaiwut
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phenolic Antioxidant ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The pod of lotus (Nelumbo nucifera Gaertn.) was value-added by using as raw material for phenolic antioxidant extraction using conventional shaking method. The pericarp part of lotus pod gave the significantly highest values of extractable phenolic content (EPC), flavonoid content and DPPH radical scavenging activity which were equal to 27.15±1.74 mg gallic acid equivalent (GAE)/g dry weight (dw), 0.59±0.04 mg quercetin equivalent (QE)/g dw and 0.65±0.02 mg trolox equivalent antioxidant capacity (TEAC)/g dw, respectively. Response surface methodology (RSM) was further employed to optimize the extraction conditions of phenolic antioxidant from the pod pericarp. Three independent variables, i.e., ethanol concentration (X1), shaking speed (X2) and extraction time (X3) were investigated through two responses of EPC and DPPH radical scavenging activity. The statistical analysis indicates that the three variables and the contribution of quadratic model had significant influence on the all responses (P<0.05). The optimum extraction condition was provided as follows: ethanol concentration 77.6%, shaking speed 223.4 rpm and extraction time 5.2 h. Under the optimized condition, the predicted values of EPC and DPPH radical scavenging activity were 30.00 mg GAE/g dw and 0.74 mg TEAC/g dw, respectively. This could be hence applied in industrial phenolic antioxidant extraction of inedible pericarp of lotus pod.

scholarly journals Optimization of Antioxidant Extraction in Hylocereus Polyrhizus Seed using Response Surface Methodology

2019 ◽  
Vol 8 (6S3) ◽  
pp. 43-47
Keyword(s):  
Response Surface Methodology ◽  
Ethanol Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging ◽  
Hylocereus Polyrhizus

Seeds of red pitaya fruit (Hylocereus polyrhizus) are commonly left underutilized in the food industry. Thus, the objective of this present study was to optimize the extraction condition for the maximum DPPH radical scavenging activity of red pitaya fruit seed extract using response surface methodology (RSM). A Central Composite design was employed to determine the effects of the selected variables, comprising extraction time (30-90 min), extraction temperature (40-80 ℃) and ethanol concentration (60-80 %) on the DPPH scavenging activities. Data were analyzed by using Design Expert (version 10.0.1, Stat-Ease, Inc., Minneapolis, MN, USA) statistical analysis software. The optimal extraction conditions for the highest antioxidant capacity were derived at 45 mins of extraction time, 70℃ extraction temperature and 80 % ethanol concentration that resulted in 92.89 % of scavenging activity. The optimized model developed was verified by comparing the predicted and experimental value of response. The result of measured response agreed well with the predicted values, demonstrating that the model can be used for optimizing the conditions of RSP extract that ensure high recovery of DPPH radical scavenging activity

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

DPPH Radical Scavenging Activity of Kaempferol

2013 ◽  
Vol 781-784 ◽  
pp. 1294-1297 ◽  
Author(s):  
Ping Liu ◽  
Yun Tao Gao ◽  
Jiao Jiao Yu ◽  
Jia Wei Cha ◽  
Dong Mei Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

scholarly journals IMPROVED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROSCOPY (HPLC/MS) METHOD FOR DETECTION OF ANTHRAQUINONES AND ANTIOXIDANT POTENTIAL DETERMINATION IN ALOE SINKATANA

2020 ◽  
Author(s):  
Asim Halfawi Shargi ◽  
Mohammed Aboied ◽  
Ibrahim ME ◽  
Fatehalrahman F Magbool
Keyword(s):  
Peer Review ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
E Mail ◽  
Dpph Radical Scavenging

Objectives:  Medicinal plants, either as an extract, pure compound or as a derivative, offer limitless opportunities for the discovery of new drugs. Sudan is a very rich source of medicinal plants which are used in the treatment of a wide range of diseases. Aloe sinkatana, has great potential to be developed as drug by pharmaceutical industries. The present study is undertaken to investigate the antioxidant potential of Aloe sinkatana by DPPH radical scavenging activity. In addition, the study also performed to explore the possibility of using HPLC-MS technique for the determination and analysis of Aloe sinkatana. Methods:  The extracts of Aloe sinkatana were analyzed for antioxidant activity by using DPPH free radical scavenging activity. The results indicated that the extracts showed a high effective free radical scavenging in the DPPH assay, also these extracts exhibited a noticeable antioxidant effect at low concentrations. Results:  During in vitro evaluation the antioxidant potential of methanolic extract was the highest, followed by aqueous extract  in DPPH radical scavenging activity. So the methanolic extract of the plant, exhibited a great antioxidant effect at 50 μg/ml which may be attributed to high phenolic content. Therefore, methanolic extract to be a more active radical scavenger than aqueous extract. The HPLC-MS analysis had shown the methanolic extract of Aloe sinkatana to be rich in the major anthraquinones and their glucosides, which revealed 9 compounds, and also UV spectroscopy detected the presence of two flavonoids. Conclusion:  The results indicated that the extracts of Aloe sinkatana  is a potential source of natural antioxidants or nutraceuticals with potential application to reduce oxidative stress with consequent health benefits. Due to stronger antioxidant potential and phytochemical composition, Aloe sinkatana could be proved as a valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals. Peer Review History: Received 26 March 2019; Revised 15 April; Accepted 4 May, Available online 15 May 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 4.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Name: Prof Cyprian Ogbonna ONYEJI Affiliation: Obafemi Awolowo University, Ile-Ife, Nigeria E-mail: [email protected]   Name: Dr. Gehan Fawzy Abdel Raoof Kandeel Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt E-mail: [email protected]   Name: Dr. Nyunaï Nyemb Affiliation: Ministry of Scientific Research and Innovation of Cameroon E-mail: [email protected]   Comments of reviewer(s): Similar Articles: ANTIHYPERGLYCEMIC AND ANTI-OXIDANT POTENTIAL OF ETHANOL EXTRACT OF VITEX THYRSIFLORA LEAVES ON DIABETIC RATS

scholarly journals Evaluation of antioxidant and antiinflammatory activity of ethanolic extracts of Polygonum senticosum in lipopolysaccharide-induced RAW 264.7 macrophages

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Chul Hwan Kim ◽  
Buyng Su Hwang ◽  
Yong Hwang ◽  
Young Taek Oh ◽  
Jin-Woo Jeong
Keyword(s):  
Antiinflammatory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw 264.7 ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Raw 264.7 Macrophages ◽  
Tnf Α ◽  
Dpph Radical Scavenging

Abstract Objectives This study aimed to examine the antioxidant activity and antiinflammatory effects of ethanol extract of Polygonum senticosum (EPS) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods Antioxidant activity of EPS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Proinflammatory markers produced by LPS-induced RAW 264.7 macrophages were quantified to assess the antiinflammatory activity of EPS. Results Our results showed that EPS significantly increased FRAP and DPPH radical-scavenging activity. Additionally, EPS reduced LPS-induced proinflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), along with proinflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-1β, without significant cytotoxicity. EPS significantly downregulated the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α, and IL-1β in LPS-stimulated RAW 264.7 macrophages. Positive correlations were noted between FRAP and DPPH radical-scavenging activity and antiinflammatory capacity. Conclusions Our results indicate that EPS downregulates the expression of pro-inflammatory mediators such as NO, PGE2, and cytokines (IL-1β and TNF-α) in LPS-stimulated RAW 264.7 macrophage cells. Further research is needed for its use as a treatment for inflammation and related diseases.

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