Sensitive Determination of Bioactive Crocin Analogues in Gardenia jasminoides Ellis Using SPE Prior to HPLC Analysis

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.303-306.654 ◽

2013 ◽

Vol 303-306 ◽

pp. 654-658

Author(s):

Xiao Ling Zhang ◽

Hui Juan Yu

Keyword(s):

Hplc Analysis ◽

Solid Phase ◽

Single Walled Carbon Nanotubes ◽

Gardenia Jasminoides ◽

Factors Influencing ◽

Efficient Extraction ◽

Sensitive Determination ◽

Walled Carbon Nanotubes ◽

As Solid Phase

In this study, the feasibility of single-walled carbon nanotubes (SWCNTs) as solid-phase extraction (SPE) adsorbent for the determination of bioactive crocin analogues in the traditional Chinese Medicine (TCM) of Gardenia Jasminoides Ellis prior to HPLC analysis was studied. Various experimental factors influencing SPE efficiency and HPLC conditions were studied. By using SWCNTs as the SPE adsorbent, the detection limit of 1.19-2.21 fmol (at S/N of 3) were achieved with the preconcentration efficiency of more than 120-folds, indicating this proposed method was very practical for increasing the sensitivity prior to HPLC analysis, and it may provides useful clue for the efficient extraction for the bioactive compounds in TCMs.

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Single-walled carbon nanotubes as solid-phase microextraction adsorbent for the determination of low-level concentrations of butyltin compounds in seawater

Analytica Chimica Acta ◽

10.1016/j.aca.2009.12.035 ◽

2010 ◽

Vol 662 (1) ◽

pp. 90-96 ◽

Cited By ~ 57

Author(s):

Noushin Rastkari ◽

Reza Ahmadkhaniha ◽

Nasrin Samadi ◽

Abbas Shafiee ◽

Masud Yunesian

Keyword(s):

Carbon Nanotubes ◽

Solid Phase Microextraction ◽

Solid Phase ◽

Single Walled Carbon Nanotubes ◽

Single Walled Carbon ◽

Low Level ◽

Walled Carbon Nanotubes ◽

Butyltin Compounds ◽

As Solid Phase

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Using Single-Walled Carbon Nanotubes as Solid-Phase Extraction Adsorbent for the Sensitive Determination of Amino Acid Neurotransmitters in Human Cerebrospinal Fluids

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.159.622 ◽

2010 ◽

Vol 159 ◽

pp. 622-627

Author(s):

Xiao Ling Zhang ◽

Qiao Yang

Keyword(s):

Carbon Nanotubes ◽

Amino Acid ◽

Solid Phase Extraction ◽

Solid Phase ◽

Single Walled Carbon Nanotubes ◽

Phase Extraction ◽

Amino Acid Neurotransmitters ◽

Walled Carbon Nanotubes ◽

As Solid Phase

Carbon nanotubes are a kind of new carbon-based nanomaterials, which have drawn great attention in many application fields including the biomarker analysis in biomedical and clinical research. In the present study, the feasibility of single-walled carbon nanotubes (SWCNTs) as solid-phase extraction (SPE) adsorbent for the determination of amino acid neurotransmitters (AANs) (glutamic, dopamine and γ-amino-n-butyric acid) in human cerebrospinal fluids (CSFs) was investigated. Various parameters affecting SPE efficiency including the eluent and its volume, adsorbent amount and sample volume were systematically studied. The acquired calibration curves were linear (r2 > 0.999) over the concentration range 10 to 500 ng per injection. By using SWCNTs as the SPE adsorbent, the detection limit of 12.9-42.5 fmol (at S/N of 3) were achieved with the preconcentration efficiency of more than 500-folds. The feasibility of the proposed method was validated by successfully applied to the measurements of ANNs in human CSFs sampled from healthy subjects and the patients with human immunodeficiency virus (HIV) infection, malignant melanoma and hypertension. This original application represents a powerful tool to study the kinetics of ANNs release by neuronal cells during neurotransmission, as well as for the understanding of the pathobiological and therapeutic basis of these remarkable molecular for diverse diseases.

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