Adsorption Applications of Bacillus sp. Biomass for Decolorization of Soluble Reactive Dyes Contaminated Solutions

2011 ◽  
Vol 71-78 ◽  
pp. 2294-2297
Author(s):  
Yan Li Mao ◽  
Yun Yu ◽  
Shi Tian Luo ◽  
Xiao Lin Lv
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus sp.were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Bacillus sp.was 4500 mg•L-1, and inoculum size of 6%(v/v), 28◦C, initial pH 7.0 and shaking speed of 160 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 4.0 g biomass /L broth was obtained. The Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 98.5 and 92.5%, respectively, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Biosorption Applications of Flavobacterium sp. Biomass for Decolorization of Soluble Reactive Light-Yellow K-4G Solutions

2011 ◽  
Vol 322 ◽  
pp. 440-443
Author(s):  
Kui Zhang ◽  
Hong Qiao Xing ◽  
Yao Qing Wang
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of flocculant by the culture of Flavobacterium sp. were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Flavobacterium sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30°C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 95.0% and 4.0 g biomass /L broth was obtained. The Flavobacterium sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G with a decolorization efficiency of 98.5, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Optimization of Removal Conditions of Soluble Reactive Dyes from Aqueous Solutions by Bacillus sp. Biomass

2012 ◽  
Vol 430-432 ◽  
pp. 395-398
Author(s):  
Gai Xia Fang ◽  
Li Hua Chen
Keyword(s):  
Energy Source ◽  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Initial Ph

The optimal conditions of by the culture of Bacillus sp.were examined, using beer wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in beer wastewater favorable for the growth of the Bacillus sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 93.8 and 95.5%, respectively, using 20 mL of the culture medium in 500 mL of 100 mg•L-1 dye solution.

Production of a Novel Biopolymer by Culture of Bacillus cereus B-11 Using Molasses Wastewater and its Use for Dye Removal

2011 ◽  
Vol 230-232 ◽  
pp. 1119-1122 ◽  
Author(s):  
Yan Li Mao ◽  
Chang Xun Tian ◽  
Ji Wei Zhu ◽  
Tai Zhi Zhang ◽  
Lv Bao Tong
Keyword(s):  
Bacillus Cereus ◽  
Culture Medium ◽  
Dye Removal ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus cereus B-11 were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the production of the biopolymer was 5000 mg•L-1, and inoculum size of 5%(v/v), 28◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 98.5% and 3.55 g biopolymer /L broth was obtained. The biopolymer was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 97.5 and 94.7%, respectively,using 25 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Sequestration of Reactive Light-Yellow K-4G by Free Mycobacterium phlei Cells and its Extracellular Polysaccharide

2012 ◽  
Vol 430-432 ◽  
pp. 150-153
Author(s):  
Yuan Hong Wang ◽  
Zhong Ya Liu ◽  
Chao Ding ◽  
Hong Qiqo Xing
Keyword(s):  
Extracellular Polysaccharide ◽  
Reactive Dye ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Light Yellow ◽  
Mycobacterium Phlei ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

Production of extracellular polysaccharide by the culture of Mycobacterium phlei were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Mycobacterium phlei cell was 5000 mg•L-1, and inoculum size of 5%(v/v), 28°C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 95.5% and 5.0 g cell/L broth was obtained. The Mycobacterium phlei cell was effective in flocculating some soluble reactive dye in aqueous solution, reactive Light-Yellow K-4G with a decolorization efficiency of 93.5%, respectively, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Production of a Novel Biopolymer by Culture of Pseudomonas fluorescens Using Brewery Wastewater and its Use for Dye Removal

2010 ◽  
Vol 171-172 ◽  
pp. 45-48 ◽  
Author(s):  
Yan Li Mao ◽  
Xiao Cun Xiao ◽  
Yan Jing Liu ◽  
Er Li Zhao ◽  
Lv Bin Zhai
Keyword(s):  
Pseudomonas Fluorescens ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Brewery Wastewater ◽  
Solution Ph ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Optimal Culture Condition

The optimal conditions of production of biopolymer by the culture of Pseudomonas fluorescens were examined, using brewery wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in brewery wastewater favorable for the production of the biopolymer was 6000 mg•L-1, and an optimal culture condition of inoculum size of 5%(v/v), 28°C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 3.67 g biopolymer /L broth was obtained. The biopolymer was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 95.8 and 96.2%, respectively，using 25 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.The decolorization efficiency was dependent on the flocculant dosage and solution pH.

Flocculating Activity Optimization of Lactobacillus paracasei subsp.paracasei L1 in Corn Liquid

2013 ◽  
Vol 850-851 ◽  
pp. 1172-1175
Author(s):  
Xiao Na Li ◽  
Xin Hua Li ◽  
Li Li Zhang ◽  
Jing Mu
Keyword(s):  
Culture Medium ◽  
Corn Starch ◽  
Culture Conditions ◽  
Lactobacillus Paracasei ◽  
Analysis Method ◽  
Medium Ph ◽  
Inoculum Concentration ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Surface Analysis Method

In order to improve the production process of corn starch and accelerate the separation of corn starch, flocculating activity of lactobacillus paracasei subsp.paracasei L1 isolated from natural fermentation of sweet potato acid liquor in corn liquid was optimized. The main influencing factors were determined by Plackett-Burman experimental design and the flocculating activity was optimized by response surface analysis method. The optimum culture conditions was: inoculum concentration 18%, lactose addition 0.2%, corn steeping time 0h, culture temperature 30°C, initial pH of the culture medium pH 6.5, culture time 36h, yeast extract addition 1%,concentration of corn thick liquid 1:3(w/v).The theoretical value of the flocculating activity was 0.1906 mg/ml and the verified value was 0.1854 mg/ml.

Production of a Bioflocculant by Penicillium Sp. HHE-P7 Using Sugarcane Molasses

2012 ◽  
Vol 518-523 ◽  
pp. 453-459
Author(s):  
Li Fan Liu
Keyword(s):  
Rotation Speed ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Medium Components ◽  
Flocculating Activity ◽  
Initial Ph

Bioflocculant MBF7 was produced by a novel bioflocculant-producing microorganism HHE-P7. In order to reduce the bioflocculant producing cost, culture experiments were conducted. The effects of medium components including carbon and nitrogen sources as well as culture conditions such as pH of molasses diluents, cultivating temperature, inoculum size were investigated. The results showed when the molasses waste was diluted at COD concentration of 2000 mg/L, the optimal culture conditions for MBF7 production by HHE-P7 were inoculum size 1% (v/v), initial pH 5, cultivating temperature 25°C at the rotation speed 150 r/min. Under such conditions, MBF7 had a flocculating activity of 83% for 5 g/L kaolin clay suspension. About 3.19 g crude bioflocculant could be recovered from 1.0 L of molasses fermentation broth.

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

scholarly journals Response Surface Methodology for the Optimization of Keratinase Production in Culture Medium Containing Feathers by Bacillus sp. UPM-AAG1

Catalysts ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 848 ◽  
Author(s):  
Aa’ishah Abdul Gafar ◽  
Mohd Ezuan Khayat ◽  
Siti Aqlima Ahmad ◽  
Nur Adeela Yasid ◽  
Mohd Yunus Shukor
Keyword(s):  
Amino Acids ◽  
Proteolytic Enzyme ◽  
Culture Medium ◽  
Essential Amino Acids ◽  
Inoculum Size ◽  
Commercial Application ◽  
Bacillus Sp ◽  
Broad Application ◽  
Feather Waste ◽  
Central Composite

Keratinase is a type of proteolytic enzyme with broad application in industry. The main objective of this work is the optimization of keratinase production from Bacillus sp. strain UPM-AAG1 using Plackett-Burman (PB) and central composite design (CCD) for parameters, such as pH, temperature, feather concentration, and inoculum size. The optimum points for temperature, pH, and inoculum and feather concentrations were 31.66 °C, 6.87, 5.01 (w/v), and 4.53 (w/v), respectively, with an optimum keratinase activity of 60.55 U/mL. The keratinase activity was further numerically optimized for commercial application. The best numerical solution recommended a pH of 5.84, temperature of 25 °C, inoculums’ size of 5.0 (v/v), feather concentration of 4.97 (w/v). Optimization resulted an activity of 56.218 U/mL with the desirability value of 0.968. Amino acid analysis profile revealed the presence of essential and non-essential amino acids. These properties make Bacillus sp. UPM-AAG1 a potential bacterium to be used locally for the production of keratinase from feather waste.

Screening of a Flocculant-Producing Strain and Optimal Conditions for Production of Bioflocculant

2011 ◽  
Vol 183-185 ◽  
pp. 829-833
Author(s):  
Jia Hong Wang ◽  
Jun Fei Wei ◽  
Hong Rui Ma
Keyword(s):  
Culture Conditions ◽  
Kaolin Clay ◽  
Optimal Conditions ◽  
Single Factor ◽  
Clay Suspension ◽  
Factors Affecting ◽  
Dilution Plate ◽  
Flocculating Activity ◽  
Initial Ph ◽  
High Possibility

A bioflocculant-producing strain M-3 with high flocculating activity was isolated from soil by conventional panel lineation and dilution-plate methods. It was preliminarily identified as Aspergillus based on its morphological and physiological characteristics of colony. The single factor experiments were conducted to optimize cultivating factors affecting flocculating activity, the optimal culture conditions were found to be as follows: glucose 10 g/L, glutamate 2 g/L, cultivating time 36 h, cultivating temperature 37 oC, shaking speed 120 r/min, initial pH 10. The strain could produce high effective flocculants with flocculating activity of 98.3% for kaolin clay suspension, which afforded high possibility of its practical use.

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