Flocculating Activity Optimization of Lactobacillus paracasei subsp.paracasei L1 in Corn Liquid

2013 ◽  
Vol 850-851 ◽  
pp. 1172-1175
Author(s):  
Xiao Na Li ◽  
Xin Hua Li ◽  
Li Li Zhang ◽  
Jing Mu
Keyword(s):  
Culture Medium ◽  
Corn Starch ◽  
Culture Conditions ◽  
Lactobacillus Paracasei ◽  
Analysis Method ◽  
Medium Ph ◽  
Inoculum Concentration ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Surface Analysis Method

In order to improve the production process of corn starch and accelerate the separation of corn starch, flocculating activity of lactobacillus paracasei subsp.paracasei L1 isolated from natural fermentation of sweet potato acid liquor in corn liquid was optimized. The main influencing factors were determined by Plackett-Burman experimental design and the flocculating activity was optimized by response surface analysis method. The optimum culture conditions was: inoculum concentration 18%, lactose addition 0.2%, corn steeping time 0h, culture temperature 30°C, initial pH of the culture medium pH 6.5, culture time 36h, yeast extract addition 1%,concentration of corn thick liquid 1:3(w/v).The theoretical value of the flocculating activity was 0.1906 mg/ml and the verified value was 0.1854 mg/ml.

Adsorption Applications of Bacillus sp. Biomass for Decolorization of Soluble Reactive Dyes Contaminated Solutions

2011 ◽  
Vol 71-78 ◽  
pp. 2294-2297
Author(s):  
Yan Li Mao ◽  
Yun Yu ◽  
Shi Tian Luo ◽  
Xiao Lin Lv
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus sp.were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Bacillus sp.was 4500 mg•L-1, and inoculum size of 6%(v/v), 28◦C, initial pH 7.0 and shaking speed of 160 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 4.0 g biomass /L broth was obtained. The Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 98.5 and 92.5%, respectively, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Biosorption Applications of Flavobacterium sp. Biomass for Decolorization of Soluble Reactive Light-Yellow K-4G Solutions

2011 ◽  
Vol 322 ◽  
pp. 440-443
Author(s):  
Kui Zhang ◽  
Hong Qiao Xing ◽  
Yao Qing Wang
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of flocculant by the culture of Flavobacterium sp. were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Flavobacterium sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30°C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 95.0% and 4.0 g biomass /L broth was obtained. The Flavobacterium sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G with a decolorization efficiency of 98.5, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Production of a Novel Biopolymer by Culture of Bacillus cereus B-11 Using Molasses Wastewater and its Use for Dye Removal

2011 ◽  
Vol 230-232 ◽  
pp. 1119-1122 ◽  
Author(s):  
Yan Li Mao ◽  
Chang Xun Tian ◽  
Ji Wei Zhu ◽  
Tai Zhi Zhang ◽  
Lv Bao Tong
Keyword(s):  
Bacillus Cereus ◽  
Culture Medium ◽  
Dye Removal ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus cereus B-11 were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the production of the biopolymer was 5000 mg•L-1, and inoculum size of 5%(v/v), 28◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 98.5% and 3.55 g biopolymer /L broth was obtained. The biopolymer was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 97.5 and 94.7%, respectively,using 25 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

scholarly journals Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Zhu Li ◽  
Lingqia Su ◽  
Xuguo Duan ◽  
Dan Wu ◽  
Jing Wu
Keyword(s):  
Culture Medium ◽  
Bacillus Stearothermophilus ◽  
Corn Starch ◽  
Expression System ◽  
Initial Ph ◽  
Brevibacillus Choshinensis ◽  
Efficient Expression ◽  
Cellular Integrity ◽  
Maltose Syrup ◽  
Starch Industry

The maltohexaose-forming, Ca2+-independent α-amylase gene from Bacillus stearothermophilus (AmyMH) was efficiently expressed in Brevibacillus choshinensis SP3. To improve the production of AmyMH in B. choshinensis SP3, the temperature and initial pH of culture medium were optimized. In addition, single-factor and response surface methodologies were pursued to optimize culture medium. Addition of proline to the culture medium significantly improved the production of recombinant α-amylase in B. choshinensis SP3. This improvement may result from improved cellular integrity of recombinant B. choshinensis SP3 in existence of proline. Culture medium optimization resulted in an 8-fold improvement in α-amylase yield, which reached 1.72 × 104 U·mL−1. The recombinant α-amylase was applied to the production of maltose on a laboratory scale. A maltose content of 90.72%, which could be classified as an extremely high maltose syrup, could be achieved using 15% (m/v) corn starch as the substrate. This study demonstrated that the B. choshinensis SP3 expression system was able to produce substantial quantities of recombinant α-amylase that has potential application in the starch industry.

scholarly journals The Growth Comparison of Haematococcus Pluvialis in Two Different Medium

Biota ◽  
2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Dina Soes Putri ◽  
Siti Alaa
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Haematococcus Pluvialis ◽  
Marine Ecosystem ◽  
Culture Conditions ◽  
Vital Role ◽  
Freshwater Microalgae ◽  
Inoculum Concentration ◽  
Chemical Factors ◽  
Physical And Chemical

Microalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on  H. pluvialis biomass is exploring its high-value bio compound. 

Production of a Novel Biopolymer by Culture of Pseudomonas fluorescens Using Brewery Wastewater and its Use for Dye Removal

2010 ◽  
Vol 171-172 ◽  
pp. 45-48 ◽  
Author(s):  
Yan Li Mao ◽  
Xiao Cun Xiao ◽  
Yan Jing Liu ◽  
Er Li Zhao ◽  
Lv Bin Zhai
Keyword(s):  
Pseudomonas Fluorescens ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Brewery Wastewater ◽  
Solution Ph ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Optimal Culture Condition

The optimal conditions of production of biopolymer by the culture of Pseudomonas fluorescens were examined, using brewery wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in brewery wastewater favorable for the production of the biopolymer was 6000 mg•L-1, and an optimal culture condition of inoculum size of 5%(v/v), 28°C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 3.67 g biopolymer /L broth was obtained. The biopolymer was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 95.8 and 96.2%, respectively，using 25 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.The decolorization efficiency was dependent on the flocculant dosage and solution pH.

Screening of a Flocculant-Producing Strain and Optimal Conditions for Production of Bioflocculant

2011 ◽  
Vol 183-185 ◽  
pp. 829-833
Author(s):  
Jia Hong Wang ◽  
Jun Fei Wei ◽  
Hong Rui Ma
Keyword(s):  
Culture Conditions ◽  
Kaolin Clay ◽  
Optimal Conditions ◽  
Single Factor ◽  
Clay Suspension ◽  
Factors Affecting ◽  
Dilution Plate ◽  
Flocculating Activity ◽  
Initial Ph ◽  
High Possibility

A bioflocculant-producing strain M-3 with high flocculating activity was isolated from soil by conventional panel lineation and dilution-plate methods. It was preliminarily identified as Aspergillus based on its morphological and physiological characteristics of colony. The single factor experiments were conducted to optimize cultivating factors affecting flocculating activity, the optimal culture conditions were found to be as follows: glucose 10 g/L, glutamate 2 g/L, cultivating time 36 h, cultivating temperature 37 oC, shaking speed 120 r/min, initial pH 10. The strain could produce high effective flocculants with flocculating activity of 98.3% for kaolin clay suspension, which afforded high possibility of its practical use.

scholarly journals Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient StrainBacillus subtilisUJS0717

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Zhuan Wei ◽  
Jue Zhou ◽  
WenJing Sun ◽  
FengJie Cui ◽  
QinHua Xu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Corn Steep Liquor ◽  
Culture Conditions ◽  
Medium Composition ◽  
Production Performance ◽  
Raw Material ◽  
Initial Ph ◽  
Fermentation Parameters ◽  
Inoculum Volume ◽  
Starch Hydrolysate

D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design. The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3. These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.

Optimization of Removal Conditions of Soluble Reactive Dyes from Aqueous Solutions by Bacillus sp. Biomass

2012 ◽  
Vol 430-432 ◽  
pp. 395-398
Author(s):  
Gai Xia Fang ◽  
Li Hua Chen
Keyword(s):  
Energy Source ◽  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Initial Ph

The optimal conditions of by the culture of Bacillus sp.were examined, using beer wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in beer wastewater favorable for the growth of the Bacillus sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 93.8 and 95.5%, respectively, using 20 mL of the culture medium in 500 mL of 100 mg•L-1 dye solution.

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