Antioxidant Defense Enzymes Response Following Polychlorinated Biphenyls Exposure to Eisenia fetida in Actual Polluted Soil

The responses of antioxidant systems after exposing Eisenia fetida to actual soil contaminated with PCBs were investigated. Among antioxidant enzymes, the primary response to early PCBs exposure can be attributed to catalase (CAT) and peroxidase (POD). These two enzymes could have a combined effect on fighting damage by reactive oxygen species. An obvious increase in the activity of CAT was recorded at 4 mg kg-1 compared to the control throughout the whole test days. PCBs exposure caused changes in POD activity in 2d and 4d. However, the activity of POD in E. fetida tissues changed little with PCBs concentrations in day 8 and 16d. Superoxide dismutase (SOD) changed little with PCBs in different time. The results suggest that the variations in CAT and POD of E. fetida could be used as early responsive biomarkers for oxidative stress caused by PCBs in a soil environment.

Download Full-text

Effects of age and strain on small intestinal and hepatic antioxidant defense enzymes in Wistar and Fisher 344 rats

Mechanisms of Ageing and Development ◽

10.1016/s0047-6374(01)00236-6 ◽

2001 ◽

Vol 122 (6) ◽

pp. 561-570 ◽

Cited By ~ 23

Author(s):

InSurk Jang ◽

KabRyong Chae ◽

JungSik Cho

Keyword(s):

Antioxidant Defense ◽

Defense Enzymes ◽

Antioxidant Defense Enzymes ◽

Small Intestinal

Download Full-text

Prospects for the use of highly effective and safe stimulants of embryogenesis in poultry farming

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202106007 ◽

2021 ◽

Vol 1 (6) ◽

pp. 42-50

Author(s):

I. I. Kochish ◽

◽

T. A. Sadovskaya ◽

T. O. Azarnova ◽

◽

...

Keyword(s):

Antioxidant Defense ◽

Morphological Changes ◽

Biologically Active ◽

Defense Enzymes ◽

Chick Embryos ◽

Negative Effects ◽

Resource Saving ◽

Antioxidant Defense Enzymes ◽

Active Substances ◽

Poultry Farming

To increase the embryonic and postembryonic viability of poultry, along with selection work, optimization of the microclimate parameters of poultry houses and rations, it is also advisable to develop resource-saving and environmentally friendly methods of using biologically active substances. It was shown that the treatment of eggs with a composition of solutions of natural metabolites (colamine, succinic acid and Ribav) in optimal concentrations stopped and prevented the negative effects of stress. Positive biochemical and morphological changes were observed in the development of chick embryos. An increase in chick withdrawal, a decrease in all incubation waste, an increase in the activity of antioxidant defense enzymes, and a decrease in the content of malondialdehyde in the blood serum of chickens were found.

Download Full-text

Molecular Role of Nitric Oxide in Secondary Products Production in Ginkgo biloba Cell Suspension Culture

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319660 ◽

2015 ◽

Vol 43 (1) ◽

pp. 12-18 ◽

Cited By ~ 5

Author(s):

Hossam Saad EL-BELTAGI ◽

Osama K. AHMED ◽

Adel E. HEGAZY

Keyword(s):

Nitric Oxide ◽

Ginkgo Biloba ◽

Antioxidant Defense ◽

Nitric Oxide Donor ◽

Peat Moss ◽

Defense Enzymes ◽

Secondary Products ◽

Equal Concentration ◽

Shoot Height ◽

Antioxidant Defense Enzymes

Effects of sodium nitroprusside (SNP; nitric oxide donor) treatment on the enhancement of secondary metabolites production, oxidative stress mediators (O2-.) accumulation and antioxidant defense enzymes of Ginkgo biloba callus culture was investigated. On one hand, the obtained data showed a highly metabolic modification of chemical constituents, PAL activity and various antioxidant defense enzymes (APX, SOD), which gradually increased in response to SNP treatments. On the other hands the high NO levels significantly increased the accumulation of various oxidative burst of O2-.. MS basal medium supplemented with casein hydrolase (500 mg/L), NAA and BA at equal concentration (0.5 mg/L) recorded the highest number of regenerated shoots (4.81 cm) and shoot height (4.96 cm) as well as root number (2.25 cm) and root length (4.5 cm). The highest survival (40 %) was shown in acclimatization on the mixture containing sand, peat moss and vermiculite (1: 1: 1, v/v/v), which significantly confirmed and reflected the variation in survival percentage. Meanwhile, higher treatment (500 μM) of NO positively enhanced secondary products accumulation of total tannins, saponins, phenols and total flavonoids in G. biloba callus culture.

Download Full-text

Biosynthetic insulin effect on red cell membrane lipid peroxidation in patients with insulin-dependent diabetes

Problems of Endocrinology ◽

10.14341/probl12003 ◽

1994 ◽

Vol 40 (3) ◽

pp. 12-15 ◽

Cited By ~ 2

Author(s):

T. S. Balashova ◽

Ye. N. Golega ◽

I. A. Rudko ◽

M. I. Balabolkin ◽

A. A. Kubatiev

Keyword(s):

Cell Membrane ◽

Human Insulin ◽

Antioxidant Defense ◽

Vascular Complications ◽

Membrane Lipid ◽

Defense Enzymes ◽

Red Cell ◽

Membrane Lipid Peroxidation ◽

Red Cell Membrane ◽

Antioxidant Defense Enzymes

The aim of our research was elucidation of a relationship between red cell membrane lipid peroxidation (LPO) and antioxidant' defense enzymes, on the one hand and the age disease duration and presence of vascular complications in patients with type I diabetes mellitus on the other. The possibility of correcting red cell peroxide status with human insulin preparations was investigated. Red cell membrane LPO was found increased more than twofold and antioxidant defense enzymes activities virtually unchanged vs. controls in 16 patients with diabetes aged 20 to 43. These characteristics of red cell peroxidation status do not depend on patients age disease standing or presence of vascular complications. A twelve-week therapy with biosynthetic insulin resulted in complete normalization of LPO processes in patients with angiopathies aged under 35 and with disease standing of less than 10 years. In diabetics with angiopathies aged over 35 and disease standing of more than 10 years red cell MDA level reduced under the effect of therapy with human insulin preparations but was still increased vs. that in healthy donors by 1.5 times. Red cell GP and SOD activities reduced in the course of insulin therapy in all the examined groups of diabetics. Catalase activity increased by approximately 50% in patients with angiopathies those aged over 35 and a disease standing of more than 10 years under the effect of insulin. In the rest groups of patients catalase activity did not differ from its initial level. Our results permit us recommending besides human insulin preparations antioxidant therapy for patients with vascular complications those aged over 35 and a disease standing of more than 10 years.

Download Full-text