Cytotoxicity Test of Brown Silk from Attacus atlas L. on Rat Smooth Muscle Cells

2015 ◽  
Vol 1112 ◽  
pp. 433-436
Author(s):  
Mokhamad Fakhrul Ulum ◽  
Deni Noviana
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Direct Method ◽  
Muscle Cells ◽  
Cytotoxicity Test ◽  
Natural Silk ◽  
Surgical Suture ◽  
Methyl Thiazolyl Tetrazolium Assay ◽  
Suture Thread ◽  
Low Cytotoxicity

Natural brown silk biomaterial from silkworm Attacus atlas L. (A. atlas L.) is a biopolymer that has many advantages compared to commercial silk from Bombyx mori (B. mori). The aim of this study was to investigate the cytotoxicity of A. atlas L. silk on rat smooth muscle cells with the direct method of methyl thiazolyl tetrazolium assay compared to commercial surgical suture silk. The result showed that brown colored natural silk has low cytotoxicity as same as commercial surgical suture silk with slightly lower cell viability. It was found that A. atlas L. silk potentially can developed to be surgical suture thread as a new expectation of biopolymer material in biomedical products.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

173: Lipid Rafts are Negative Regulators of TGFβ Signaling in Ureteral Smooth Muscle Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 46-46
Author(s):  
Carlos R. Estrada ◽  
Theodora Danciu ◽  
Maximilian Stehr ◽  
Joseph Khoury ◽  
Keith R. Solomon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Lipid Rafts ◽  
Muscle Cells ◽  
Negative Regulators ◽  
Tgfβ Signaling

1708: Transfection of Bladder Smooth Muscle Cells with Telokin Alters their Smooth Muscle Phenotype

2004 ◽  
Vol 171 (4S) ◽  
pp. 452-452
Author(s):  
Grace Hyun ◽  
Shaohua Chang ◽  
Stephen A. Zderic ◽  
Alan J. Wein ◽  
Samuel Chacko ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bladder Smooth Muscle
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