Epipremnum pinnatum Activity on Chick Pancreatic Lipase

Epipremnum pinnatum is a vegetative, type of vines and climbing plant that can be found in a semitropical and tropical country in South East Asia and Asian region. It has been used as folk remedies due to its medicinal properties and capability in enhancing the immune system. However, limited scientific data are present to support this claim. Thus, the present study was focused on the determination of phytochemical properties of the extract E. pinnatum and the effect on the pancreatic lipase inhibitory activities. Phytochemical of E. pinnatum was extracted using supercritical carbon dioxide (CO2) at 6000 psi and 40°C. The extracted oil was analyzed using Follin- Ciocalteu method to determine the phenolic components and followed by gas chromatography analysis. Chicken pancreas was used to investigate pancreatic lipase inhibitory activities of the extracted E.pinnatum. The results indicated that extract E.pinnatum could inhibit the chicken pancreatic lipase activity and based on the analysis obtained, the amount of lipase activity inhibited was 74 % by using 48 μg/ml of extract E.pinnatum.

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Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Foods ◽

10.3390/foods9070844 ◽

2020 ◽

Vol 9 (7) ◽

pp. 844

Author(s):

Razia Sultana ◽

Adeola M. Alashi ◽

Khaleda Islam ◽

Md Saifullah ◽

C. Emdad Haque ◽

...

Keyword(s):

Angiotensin Converting Enzyme ◽

Pancreatic Lipase ◽

Lipase Activity ◽

Inhibitory Activity ◽

The Other ◽

Converting Enzyme ◽

Inhibitory Activities ◽

Ash Gourd ◽

Better Than

The aim of the study was to determine the in vitro enzyme inhibition activities of aqueous polyphenolic extracts of nine popular Bangladeshi vegetables, namely ash gourd, bitter gourd, brinjal, Indian spinach, kangkong, okra, ridge gourd, snake gourd, and stem amaranth. Polyphenolic glycosides were the major compounds present in the extracts. Inhibition of α-amylase (up to 100% at 1 mg/mL) was stronger than α-glucosidase inhibition (up to 70.78% at 10 mg/mL). The Indian spinach extract was the strongest inhibitor of pancreatic lipase activity (IC50 = 276.77 µg/mL), which was significantly better than that of orlistat (381.16 µg/mL), a drug. Ash gourd (76.51%), brinjal (72.48%), and snake gourd (66.82%) extracts were the most effective inhibitors of angiotensin-converting enzyme (ACE), an enzyme whose excessive activities have been associated with hypertension. Brinjal also had a significantly higher renin-inhibitory activity than the other vegetable extracts. We conclude that the vegetable extracts may have the ability to reduce enzyme activities that have been associated with hyperglycemia, hyperlipidemia, and hypertension.

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Isolation of Polyphenol Compounds from Ganoderma lucidum and Pancreatic Lipase Inhibitory Activities

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2020.49.1.28 ◽

2020 ◽

Vol 49 (1) ◽

pp. 28-34

Author(s):

Eun-Ah Ryu ◽

Ji-Hye Choi ◽

Gi-Un Seong ◽

Shin-Kyo Chung

Keyword(s):

Pancreatic Lipase ◽

Ganoderma Lucidum ◽

Polyphenol Compounds ◽

Inhibitory Activities

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Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

Molecules ◽

10.3390/molecules26072075 ◽

2021 ◽

Vol 26 (7) ◽

pp. 2075

Author(s):

Tianrui Zhao ◽

Mengxue Sun ◽

Lingpeng Kong ◽

Qingwang Xue ◽

Yudan Wang ◽

...

Keyword(s):

Pancreatic Lipase ◽

Antioxidant Activities ◽

Bioactive Components ◽

Inhibitory Effects ◽

Macroporous Resin ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ic50 Values ◽

Trolox Equivalent ◽

Inhibitory Activities

Vaccinium dunalianum Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of V.dunalianum extract and isolate the bioactive components. In this study, the crude extract (CE) from the buds of V. dunalianum was prepared by the ultrasound-assisted extraction method in 70% methanol and then purified with macroporous resin D101 to obtain the purified extract (PM). Five fractions (Fr. A–E) were further obtained by MPLC column (RP-C18). Bioactivity assays revealed that Fr. B with 40% methanol and Fr. D with 80% methanol had better antioxidant with 0.48 ± 0.03 and 0.62 ± 0.01 nM Trolox equivalent (TE)/mg extract for DPPH, 0.87 ± 0.02 and 1.58 ± 0.02 nM TE/mg extract for FRAP, 14.42 ± 0.41 and 19.25 ± 0.23 nM TE/mg extract for ABTS, and enzyme inhibitory effects with IC50 values of 95.21 ± 2.21 and 74.55 ± 3.85 for α-glucosidase, and 142.53 ± 11.45 and 128.76 ± 13.85 µg/mL for pancreatic lipase. Multivariate analysis indicated that the TPC and TFC were positively related to the antioxidant activities. Further phytochemical purification led to the isolation of ten compounds (1–10). 6-O-Caffeoylarbutin (7) showed significant inhibitory effects on α-glucosidase and pancreatic lipase enzymes with values of 38.38 ± 1.84 and 97.56 ± 7.53 µg/mL, and had the highest antioxidant capacity compared to the other compounds.

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Modification of a phospholipid stabilized emulsion interface by bile salt: effect on pancreatic lipase activity

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)33300-9 ◽

1998 ◽

Vol 39 (3) ◽

pp. 623-632 ◽

Cited By ~ 7

Author(s):

Martin Wickham ◽

Martin Garrood ◽

John Leney ◽

Peter D.G. Wilson ◽

Annette Fillery-Travis

Keyword(s):

Bile Salt ◽

Pancreatic Lipase ◽

Lipase Activity ◽

Salt Effect

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Diversity of Root Bacteria from Peanut Cropping Systems

Peanut Science ◽

10.3146/pnut.31.2.0005 ◽

2004 ◽

Vol 31 (2) ◽

pp. 86-91 ◽

Cited By ~ 2

Author(s):

D. T. Gooden ◽

H. D. Skipper ◽

J. H. Kim ◽

K. Xiong

Keyword(s):

Fatty Acids ◽

Biological Control ◽

Gas Chromatography ◽

Cropping Systems ◽

Cropping System ◽

Critical Research ◽

Chromatography Analysis ◽

Rotation System ◽

Gas Chromatography Analysis ◽

Peanut Production

Abstract Rhizobacteria play an important role in sustainable agriculture via plant growth and biological control of pests in a number of ecosystems. Understanding the interactions of crop rotation and rhizobacteria on peanut production is a critical research need. Development of a database on the rhizobacteria obtained from continuous and rotational fields of peanut was initiated in 1997 and terminated in 2000. Peanut was planted in monoculture for 4 yr. In rotational plots, peanut, cotton, corn, and peanut were planted in sequence. Rhizobacteria were isolated from the roots of crop plants grown in a Norfolk soil near Florence, SC. These isolates were identified by composition of fatty acids from gas chromatography analysis (GC/FAME). Arthrobacter and Bacillus were the major genera from non-rhizosphere soils. At initiation of this study in July 1997, the plots selected for continuous peanut had more diversity in rhizobacteria than those plots selected for rotation. In July 2000, rhizobacteria diversity was greater from peanut roots in the rotation cropping system than continuous peanut. Even though rhizobacteria diversity was greater in the rotation system, higher peanut yields were recorded in the continuous peanut system in 2000. Burkholderia spp. were always isolated from the peanut and other crop rhizospheres at each sampling date.

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