Analysis of Acidic End Products and Molecular Characterization of Biohydrogenbacterium R3 sp.nov

Biohydrogenbacterium R3 sp.nov, as a typical strains of hydrogen production bacteria, was isolated from the activated sludge in the biohydrogen production reaction by using culture media of HPB-LR which was designed particularly for isolating anaerobic fermentation bacteria and an improved Hungate rolling tubes technique and a plate method of culture bottle (PMCB) which were used for counting and isolating the anaerobes by our lab. Two representative strains of hydrogen production bacteria, namely RL20, RL37, and a strain of no hydrogen production bacterium, namely RL16 were used as contrast analysis. Experiment results indicated that the distributed proportion of ethanol and acetic acid was 95%-99% in the total amount of VAFs (Volatile Fatty Acid) of R3 which were typical ethanol-type fermentation, and the total amount of ethanol and acetic acid was commensurate with that of propionic acid.

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The New Hydrogenogen and its Industrial Application in Molasses Fermentation System

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.542 ◽

2011 ◽

Vol 183-185 ◽

pp. 542-546

Author(s):

Jing Wei Zhang ◽

Wei Han ◽

Jian Yu Yang ◽

Zhan Qing Wang ◽

Yong Juan Zhang ◽

...

Keyword(s):

Acetic Acid ◽

Hydrogen Production ◽

Production Technology ◽

Anaerobic Fermentation ◽

Industrial Applications ◽

Hydrogen Metabolism ◽

Product Analysis ◽

Flat Panel ◽

Fermentation System ◽

Fermentation Liquid

The important premise for hydrogen production technology of fermentation generator was to separate and identify the efficient hydrogen producing bacteria. By use of Hungater technology and flat-panel technology with wide-body narrow-neck flask, LM-1 and HPB-LR medium to separate and identify the anaerobic fermentation hydrogen production bacteria, and five hydrogen-producing bacteria were obtained, whose hydrogen metabolism was ethanol fermentation. The product analysis on the hydrogen production bacteria fermentation liquid showed that ethanol and acetic acid accounted for 95%-99% of the total metabolites. These strains can be fermented to produce hydrogen, and can be used for industrial applications.

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Construction of plastic contact deformation maps on ceramics: a case study on aluminum nitride

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100165641 ◽

1996 ◽

Vol 54 ◽

pp. 636-637

Author(s):

D. L. Callahan

Keyword(s):

Plastic Deformation ◽

Aluminum Nitride ◽

Mechanical Response ◽

Three Dimensional ◽

Bright Field Image ◽

Perfectly Plastic ◽

Contrast Analysis ◽

Deformation Patterns

Modern polishing, precision machining and microindentation techniques allow the processing and mechanical characterization of ceramics at nanometric scales and within entirely plastic deformation regimes. The mechanical response of most ceramics to such highly constrained contact is not predictable from macroscopic properties and the microstructural deformation patterns have proven difficult to characterize by the application of any individual technique. In this study, TEM techniques of contrast analysis and CBED are combined with stereographic analysis to construct a three-dimensional microstructure deformation map of the surface of a perfectly plastic microindentation on macroscopically brittle aluminum nitride.The bright field image in Figure 1 shows a lg Vickers microindentation contained within a single AlN grain far from any boundaries. High densities of dislocations are evident, particularly near facet edges but are not individually resolvable. The prominent bend contours also indicate the severity of plastic deformation. Figure 2 is a selected area diffraction pattern covering the entire indentation area.

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Microbial and functional characterization of an allochthonous consortium applied to hydrogen production from Citrus Peel Waste in batch reactor in optimized conditions

Journal of Environmental Management ◽

10.1016/j.jenvman.2021.112631 ◽

2021 ◽

Vol 291 ◽

pp. 112631

Author(s):

Franciele Pereira Camargo ◽

Isabel Kimiko Sakamoto ◽

Tiago Palladino Delforno ◽

Mahendra Mariadassou ◽

Valentin Loux ◽

...

Keyword(s):

Hydrogen Production ◽

Batch Reactor ◽

Functional Characterization ◽

Citrus Peel ◽

Optimized Conditions

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Characterization of 1, 3-β-glucanases produced during autolysis of Penicillium oxalicum in different culture media

Transactions of the British Mycological Society ◽

10.1016/s0007-1536(87)80004-9 ◽

1987 ◽

Vol 88 (3) ◽

pp. 317-321 ◽

Cited By ~ 10

Author(s):

J.L. Copa-Patiño ◽

I.F. Monistrol ◽

F. Laborda ◽

M.I. Pérez-Leblic

Keyword(s):

Culture Media ◽

Penicillium Oxalicum

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In Vitro Characterization of the Enzyme Properties of the Phospholipid N-Methyltransferase PmtA from Agrobacterium tumefaciens

Journal of Bacteriology ◽

10.1128/jb.01591-08 ◽

2009 ◽

Vol 191 (7) ◽

pp. 2033-2041 ◽

Cited By ~ 18

Author(s):

Meriyem Aktas ◽

Franz Narberhaus

Keyword(s):

Agrobacterium Tumefaciens ◽

In Vitro Assay ◽

Enzyme Properties ◽

Vitro Assay ◽

Plant Infection ◽

In Vitro Characterization ◽

End Products ◽

And Control

ABSTRACT Agrobacterium tumefaciens requires phosphatidylcholine (PC) in its membranes for plant infection. The phospholipid N-methyltransferase PmtA catalyzes all three transmethylation reactions of phosphatidylethanolamine (PE) to PC via the intermediates monomethylphosphatidylethanolamine (MMPE) and dimethylphosphatidylethanolamine (DMPE). The enzyme uses S-adenosylmethionine (SAM) as the methyl donor, converting it to S-adenosylhomocysteine (SAH). Little is known about the activity of bacterial Pmt enzymes, since PC biosynthesis in prokaryotes is rare. In this article, we present the purification and in vitro characterization of A. tumefaciens PmtA, which is a monomeric protein. It binds to PE, the intermediates MMPE and DMPE, the end product PC, and phosphatidylglycerol (PG) and phosphatidylinositol. Binding of the phospholipid substrates precedes binding of SAM. We used a coupled in vitro assay system to demonstrate the enzymatic activity of PmtA and to show that PmtA is inhibited by the end products PC and SAH and the antibiotic sinefungin. The presence of PG stimulates PmtA activity. Our study provides insights into the catalysis and control of a bacterial phospholipid N-methyltransferase.

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Synthesis and Characterization of Thiophen-3-yl Acetic Acid 4-Pyrrol-1-yl Phenyl Ester and its Conducting Polymers

International Journal of Polymeric Materials ◽

10.1080/00914030490462008 ◽

2005 ◽

Vol 54 (8) ◽

pp. 713-730 ◽

Cited By ~ 3

Author(s):

Bahar Bingöl ◽

Yusuf Güner ◽

Ali Çirpan ◽

Levent Toppare

Keyword(s):

Acetic Acid ◽

Conducting Polymers ◽

Synthesis And Characterization ◽

Phenyl Ester

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Preparation and Characterization of Polyaniline Nanotubes Doped with Different Acid

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.401-403.663 ◽

2013 ◽

Vol 401-403 ◽

pp. 663-666

Author(s):

Xue Lian Bai ◽

Jian Ting Mei ◽

Zhong Guo Mu ◽

Yun Bai

Keyword(s):

Acetic Acid ◽

Self Assembly ◽

Ammonium Persulfate ◽

Tetraacetic Acid ◽

X Ray Diffraction ◽

X Ray ◽

Structure And Property ◽

Assembly Method ◽

Polyaniline Nanotubes

Polyaniline (PANI) nanotubes were synthesized separately using amino acetic acid (AA), ethylenediamine tetraacetic acid (EDTA), oxalic acid (OA) as dopant and ammonium persulfate (APS) as oxidant by a self-assembly method. SEM, TEM,FTIR and X-ray diffraction (XRD) and applying the 4 probes method characterized the morphology, structure and property of the product. It was found that nanotubes morphology were synthesized when the [Aci/[A ratio is 1:2.The room template conductivity of the products were studied.

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