Characterization of 1, 3-β-glucanases produced during autolysis of Penicillium oxalicum in different culture media

The study was intended for molecular detection of S. aureus isolated from raw cows milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

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Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽

10.1007/s11306-016-1029-2 ◽

2016 ◽

Vol 12 (5) ◽

Cited By ~ 4

Author(s):

Érika Cristina dos Santos ◽

Camila Bruna de Lima ◽

Kelly Annes ◽

Marcella Pecora Milazzotto

Keyword(s):

Culture Media ◽

Bovine Embryos ◽

In Vitro Production ◽

Noninvasive Characterization ◽

Vitro Production

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Short Communication: Characterization of Rhizoctonia-like mycorrhizae associated with five Dendrobium species in Java, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210321 ◽

2020 ◽

Vol 21 (3) ◽

Author(s):

R Soelistijono ◽

D.S. UTAMI ◽

DARYANTI ◽

M. FAIZIN ◽

R. DIAN

Keyword(s):

Short Communication ◽

Culture Media ◽

Dry Season ◽

The Other ◽

Root Tissue ◽

Binucleate Rhizoctonia ◽

Anatomical Characteristics ◽

Macroscopic Observation

Abstract. Soelistijono R, Utami DS, Daryanti, Faizin M, Dian R. 2020. Plankton biodiversity in various typologies of inundation in Paminggir swamp, South Kalimantan, Indonesia on dry season. Biodiversitas 21: 1007-1011. This study aims to determine the morphological and anatomical characteristics of Rhizoctonia-like mycorrhizae associated with the roots of five Dendrobium species; to determine the association between Rhizoctonia-like mycorrhizae with the root of five Dendrobium sp.; to obtain difference between Rhizoctonia-like mycorrhizae with the other in adjacent location Mycorrhizal observations of Rhizoctonia-like mycorrhizae in this study were carried out macroscopically (morphologically) and microscopically (anatomically). The macroscopic observation was performed by observing directly the development of fungal colonies on culture media. Microscopic observations were performed to determine the shape of the hyphal of fungi and the number of nuclei. The results showed that the Rhizoctonia-like mycorrhizae associated with the root of five species of Dendrobium sp. in Java were the binucleate Rhizoctonia groups (BNR). The binucleate Rhizoctonia has white colonies, right-angle branching hyphae, two nuclei, and brown hyphae. The association of the root of five species of Dendrobium sp.with Rhizoctonia-like mycorrhizae fungi was indicated by the existence of a peloton structure in cortical root tissue.

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Production and characterization of biomass and exopolysaccharides obtained in submerged culture under different initial pHs used in the cultivation of Colletotrichum gloeosporioides and Rhizopus stolonifer

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i8.2139 ◽

2020 ◽

pp. 628

Author(s):

Juan Diego Valenzuela Cobos ◽

René Oscar Rodríguez-Grimón ◽

Ana Grijalva-Endara ◽

Raúl Marcillo-Vallejo ◽

Onay Adonys Mercader-Camejo

Keyword(s):

Yeast Extract ◽

Submerged Culture ◽

Liquid Culture ◽

Colletotrichum Gloeosporioides ◽

Culture Media ◽

Mycelial Biomass ◽

Rhizopus Stolonifer

Colletotrichum gloeosporioides (GC003) and Rhizopus stolonifer (RS001) were cultivated in two different liquid culture media: LC1 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 2 g L-1) and LC2 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 10 g L-1) for the production of mycelial biomass and exopolysaccharides (EPS). By using the liquid culture (LC2) under pH of 4.5 presented the highest biomass content (15.73 g L-1) in the propagation of Rhizopus stolonifer. The highest production of exopolysaccharides (1.74 g L-1) was obtained by the liquid culture (LC2) under pH of 4.5 in the cultivation of Colletotrichum gloeosporioides. The results presented that the production of biomass and exopolysaccharides (EPS) is directly related with the pHs values and the strain used in the cultivation.

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Biodegradation of Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Newly Isolated Penicillium oxalicum SS2 in Soil Microcosms and Partial Characterization of Extracellular Depolymerase

Current Microbiology ◽

10.1007/s00284-020-01968-7 ◽

2020 ◽

Vol 77 (8) ◽

pp. 1622-1636

Author(s):

Sadia Mehmood Satti ◽

Ziaullah Shah ◽

Aaisha Luqman ◽

Fariha Hasan ◽

Muhammad Osman ◽

...

Keyword(s):

Penicillium Oxalicum ◽

Partial Characterization ◽

Soil Microcosms

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Characterization of Biomimetic Mineral Coated 3D PLGA Scaffolds

Materials, Nondestructive Evaluation, and Pressure Vessels and Piping ◽

10.1115/imece2006-14877 ◽

2006 ◽

Author(s):

A. Champa Jayasuriya ◽

Chiragkumar Shah ◽

Vijay Goel ◽

Nabil A. Ebraheim

Keyword(s):

Cell Culture ◽

Culture Media ◽

Biological Molecules ◽

Polymer Surfaces ◽

Human Blood Plasma ◽

Carbonate Apatite ◽

X Ray Diffraction ◽

Cell Culture Media ◽

Salt Leaching

The bone-like carbonate apatite (BLCA) coatings can be coated biomimetically in the polymer surfaces by soaking in the simulated body fluid (SBF). This SBF contains similar ionic constituents to human blood plasma. Micro-porous 3D poly(lactic-co-glycolic acid) PLGA scaffolds were fabricated by the solvent casting/salt leaching technique using chloroform to dissolve the polymer. We accelerated the deposition of mineral on scaffolds for 1-2 days, modifying the mineralization process using surface treatments and 5x SBF. These scaffolds were analyzed by Scanning Electron Microscopy (SEM), Fourier Transform Infra-Red (FTIR) and X-ray Diffraction (XRD). The scaffolds coated with BLCA layer were placed in the 24 well plates containing 2 ml of media, such as Tris Buffered Saline-pH 7.4, cell culture media containing αMEM supplemented with 10% FBS, and 1% penicillin-streptomycin and incubated at 37°C for 21 days. The BLCA layer on surfaces of scaffold was stable even after 21 days immersed in Tris Buffered Saline and cell culture media. This study suggests that BLCA were stable for at least 3 weeks in the both media, and therefore, mineral has a potential to use as a carrier for biological molecules for localized release applications as well as bone tissue engineering applications.

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Applicability and Limitations in the Characterization of Poly-Dispersed Engineered Nanomaterials in Cell Media by Dynamic Light Scattering (DLS)

Materials ◽

10.3390/ma12233833 ◽

2019 ◽

Vol 12 (23) ◽

pp. 3833 ◽

Cited By ~ 2

Author(s):

Arianna Marucco ◽

Elisabetta Aldieri ◽

Riccardo Leinardi ◽

Enrico Bergamaschi ◽

Chiara Riganti ◽

...

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Culture Media ◽

Raw 264.7 ◽

Pre Treatment ◽

The Stability ◽

Definition Of ◽

Cellular Tests

The dispersion protocol used to administer nanomaterials (NMs) in in vitro cellular tests might affect their toxicity. For this reason, several dispersion procedures have been proposed to harmonize the toxicological methods, allowing for the comparison of the data that were obtained by different laboratories. At the same time, several techniques and methods are available to monitor the identity of the NMs in the cell media. However, while the characterization of suspensions of engineered NMs having narrow size distribution may be easily performed, the description of aggregated NMs forming polydispersions is still challenging. In the present study, sub-micrometric/nanometric TiO2, SiO2, and CeO2 were dispersed in cell media by using two different dispersion protocols, with and without albumin (0.5%) and with different sonication procedures. Dynamic Light Scattering (DLS) was used to characterize NMs in stock solutions and culture media. Pitfalls that affect DLS measurements were identified and, guidance on a critical analysis of the results provided. The NMs were then tested for their cytotoxicity (LDH leakage) toward murine macrophages (RAW 264.7) and PMA-activated human monocytes (THP-1). As markers of pro-inflammatory response, nitric oxide (NO) and cytokine IL-1β production were measured on RAW 264.7 and THP-1 cells, respectively. The pre-treatment with albumin added to a strong sonication treatment increases the stability and homogeneity of the suspensions of nanometric samples, but not of the submicrometric-samples. Nevertheless, while TiO2 and CeO2 were non-cytotoxic in any conditions, differences in cytotoxicity, NO, and IL-1β releases were found for the SiO2, depending upon the protocol. Overall, the results suggest that there is no one-fits-all method valid for all NMs, since each class of NMs respond differently. The definition of validated procedures and parameters for the selection of the most appropriate method of dispersion for each class of NM appears to be a more efficacious strategy for the harmonization of the dispersion protocols.

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Site-specific N-glycosylation of HeLa cell glycoproteins

Scientific Reports ◽

10.1038/s41598-019-51428-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Lilla Turiák ◽

Simon Sugár ◽

András Ács ◽

Gábor Tóth ◽

Ágnes Gömöry ◽

...

Keyword(s):

Hela Cell ◽

Cell Line ◽

Bovine Serum ◽

Culture Media ◽

Cancer Cell Line ◽

Hela Cell Line ◽

Glycosylation Pattern ◽

Site Specific ◽

Glycosylation Sites

Abstract We have characterized site-specific N-glycosylation of the HeLa cell line glycoproteins, using a complex workflow based on high and low energy tandem mass spectrometry of glycopeptides. The objective was to obtain highly reliable data on common glycoforms, so rigorous data evaluation was performed. The analysis revealed the presence of a high amount of bovine serum contaminants originating from the cell culture media – nearly 50% of all glycans were of bovine origin. Unaccounted, the presence of bovine serum components causes major bias in the human cellular glycosylation pattern; as is shown when literature results using released glycan analysis are compared. We have reliably identified 43 (human) glycoproteins, 69 N-glycosylation sites, and 178 glycoforms. HeLa glycoproteins were found to be highly (68.7%) fucosylated. A medium degree of sialylation was observed, on average 46.8% of possible sialylation sites were occupied. High-mannose sugars were expressed in large amounts, as expected in the case of a cancer cell line. Glycosylation in HeLa cells is highly variable. It is markedly different not only on various proteins but also at the different glycosylation sites of the same protein. Our method enabled the detailed characterization of site-specific N-glycosylation of several glycoproteins expressed in HeLa cell line.

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