The Effect of T-7 Peptide on Human Non-Small-Cell Carcinoma A549 Cells

2013 ◽  
Vol 641-642 ◽  
pp. 820-823
Author(s):  
Shu Jing Wang ◽  
Shan Jiang ◽  
Jia Liu ◽  
Fei Wang ◽  
Ning Chen ◽  
...  

7 peptide of Tumstatin(T-7 peptide) is composed of 185-191 amino acids. To study T-7 peptide antitumor activity to human non-small-cell carcinoma(A549), T-7 peptide was designed and synthesized by amino acid synthesizer. Its purity ran up to 98.45% by HPLC and MS. The effect of T-7 peptide on A549 cell growth was observed by MTT assay, growth curve and transmission electron microscopy(TEM). T-7 peptide had the effects of suppressing A549 cell growth and promoting its apoptosis, showing dose- and time-dependent. Its IC50 was 92.84 μg/ml. TEM also revealed that A549 cell treated with T-7 peptide appeared apoptotic morphology,such as cell pyknosis and mitochondrial vacuoles formed. While T-7 peptide had little effect on human umbilical vein endothelial cells(ECV304). These researches were significant to treat human non-small-cell carcinoma in the future.

Author(s):  
Jong Chul Hong ◽  
Seo Hee Rha ◽  
Hyun-Jik Lee ◽  
Heon Soo Park

2006 ◽  
Vol 55 (5) ◽  
pp. 477
Author(s):  
In Jae Lee ◽  
Kwang Seok Eom ◽  
Seon Young Jeon ◽  
Im Kyung Hwang ◽  
Yul Lee ◽  
...  

Blood ◽  
1979 ◽  
Vol 53 (4) ◽  
pp. 677-686 ◽  
Author(s):  
DC Ihde ◽  
EB Simms ◽  
MJ Matthews ◽  
MH Cohen ◽  
PA Bunn ◽  
...  

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