Enzymolysis Optimization of Walnut Residue Protein Using Trypsase

2013 ◽  
Vol 690-693 ◽  
pp. 1203-1207 ◽  
Author(s):  
Shao Ying Zhang ◽  
Yin Zhe Ren

The enzymolysis of walnut residue protein was investigated using trypsase, and the best process conditions were studied by one-factor experiment and orthogonal experiment. The result showed that the influential degree weakened as follows sequence: temperature, pH, enzyme quantity, and time. The optimum conditions were temperature 53 °C, pH 8.3, enzyme quantity 0.85%, and time 2.5 h. The enzymolysis liquid obtained was thickly fragrant, nigger-brown, and not bitter. Its ammonia-nitrogen content was 6.98 g/100 g, and hydrolysis degree was 15.76%.

2011 ◽  
Vol 331 ◽  
pp. 261-264 ◽  
Author(s):  
Qi Ming Zhao ◽  
Shan Yan Zhang

The auxiliary devices of ultrasonic treatment was designed and manufactured. The cotton fabric was desized using 2000L desizing enzyme with the conventional enzyme desizing process and ultrasonic enzyme desizing process respectively. Through the orthogonal experiment, the optimum process conditions of conventional enzyme desizing process and ultrasonic enzyme desizing process were determined. For the conventional enzyme desizing process, the optimized desizing conditions of cotton fabrics were: desizing enzyme dosage was 1.5g/l, temperature was 80°C, PH value was 6, and time was 60mins. The optimum process conditions of ultrasonic enzyme desizing process were: desizing enzyme dosage was 1.5g/l, temperature was 50°C, PH value was 6 and time was 45minutes. The research result indicates that, under the same desizing condition, ultrasonication can improve the desizing percentage and whiteness of cotton fabric, but the fabric strength loss increases slightly. And for the same required desizing percentage, the ultrasonic enzyme desizing process saved time and reduced the temperature of experiments compared with traditional enzyme desizing process


2011 ◽  
Vol 396-398 ◽  
pp. 1180-1183
Author(s):  
He Ping Li ◽  
Hu Qiang Lv ◽  
Yao Zhang ◽  
Yong Zhe Yang

In the normal pressure and presence of catalyst, a novel composite modified starch or functional surfactant was synthesized by using the cassava starch as the main feedstock through the alkalization, etherification and oxidation etc.. The degree of substitution was up to 1.21. Based on the orthogonal experiment and computer-aid methods, the optimum conditions were resulted as follows: the mass fractions(vs. dry starch) of chloroacetic acid, catalyst and oxidant were 32%, 0.01% and 1.14% respectively, and the reaction temperature of etherification was at 50°C for 4.1h. The structural characterization of the composite modified starch was determined by IR and X-ray diffraction.


2011 ◽  
Vol 55-57 ◽  
pp. 789-795
Author(s):  
Xiu Ju Duan ◽  
Qiang He ◽  
Ya Li Liu

This thesis put forward the treatment concept of “without Biomass Retention Sequential Batch Intensified Pretreatment (WSIP)” in leachate treatment, for sake of improving performance of nitrogen removal, optimizing excess water’s nutritional ratio and benefitting the follow-up aerobic biological treatment. Based on orthogonal experiment of WSIP Reactor’s leachate treatment performance, Conclusions can be drew: the removal performance of ammonia nitrogen and TN is higher of WSIP, in which short-cut nitrification and denitrification can be realized; HRT, DO and sequential period are remarkable factors of ammonia removal performance, TN removal performance and realization of short-cut nitrification and denitrification; In normal temperature, the most perfect functional parameter of WSIP Reactor is: HRT=4d, DO=0.75mg/L and sequential period is 6h.


2021 ◽  
Vol 251 ◽  
pp. 02058
Author(s):  
Feng Xuehua ◽  
Tao Ali ◽  
Song Zurong ◽  
Gong Panpan

The aqueous enzymatic method was applied to extract the common pistache oil and the optimal extraction process conditions were identified. By observing the effect of enzymatic hydrolysis time, pH value, temperature on aqueous enzymatic extraction process and performing the orthogonal experiment based on the single factor test, the optimal process parameters were obtained, namely, the optimal time, temperature, and pH value were respectively 3 h, 50℃, and 7 with a final extraction rate of 25.38 %.


2013 ◽  
Vol 726-731 ◽  
pp. 2829-2832
Author(s):  
Yong Shu Tian ◽  
Run Xin Hou

Photocatalyst of Fe-Sm-Yb modified TiO2 loaded on clay brick was prepared for degradation of NH3-N in coking water. The influence factors of NH3-N degradation rates were discussed by single factor experiment and orthogonal experiment. The optimum process conditions of photocatalytic degradation NH3-N in coking water were confirmed. The results show that NH3-N degradation rates 82.23% were highest in the conditions of pH 6.5, photocatalyst charge of 1.4 g, illumination for 6h, reaction at 55°C, calcination at 500°C.


2021 ◽  
Vol 1033 ◽  
pp. 103-108
Author(s):  
Ying Cai ◽  
Zhan Xia Wu ◽  
Zhao Yu Zhang ◽  
Zhang Hu

To meet the requirement for high transparency of agar gel used in tissue culture, bacteriological applications, and high-quality cosmetics and food, this study on improving the transparency of agar products was carried out by using activated carbon for decolorization and perlite for aiding filtration. The results showed that the pre-coating filtration method was better than that of the mixed slurry filtration, so an orthogonal experiment optimization was conducted using the pre-coating filtration method based on the single factor experimental results. The experimental results showed that the optimal process conditions based on the transparency index were: concentration of agar of 1.30%, activated carbon dosage of 0.80%, processing temperature of 95 °C, and treatment time of 20 min. Agar products with gel transparency of 58.93% and blue-ray whiteness value of 93.82 could be obtained under these experimental conditions. The order of the factors influencing the decolorization ratio of agar was as follows: activated carbon dosage > concentration of agar > processing temperature > treatment time. The experimental results provided effective procession methods for the production enterprises based on transparency and increased economic benefits, and it was of practical significance.


2013 ◽  
Vol 634-638 ◽  
pp. 349-352 ◽  
Author(s):  
Zheng Yang An ◽  
Xiao Jun Xu

A self-made coagulant was applied in the treatment of some membrane concentrated reject of the “young” landfill leachate. The optimum operation diameters were determined by orthogonal and single factor experiments. Under the optimum conditions, the values of COD and ammonia nitrogen of water sample after treatment were 88 mg/L and 10.8 mg/L, respectively, which reached the standard in Pollution Control Standard of Domestic Refuse Landfill GB 16889-2008. Therefore, coagulation method can be applied in the treatment of membrane concentrated “young” landfill leachate to achieve standard discharge.


1983 ◽  
Vol 29 (5) ◽  
pp. 867-869 ◽  
Author(s):  
R J Cooke ◽  
R L Jensen

Abstract A micromethod for measuring the ammonia nitrogen content of plasma by use of an ion-selective electrode is evaluated and described. The effect of storing plasma for 24, 48, and 72 h at -70 degrees C was evaluated. Values for newborns and fasting adults were 880 micrograms/L (SD, 210 micrograms/L) and 620 micrograms/L (SD, 170 micrograms/L), respectively.


2012 ◽  
Vol 550-553 ◽  
pp. 3323-3330 ◽  
Author(s):  
Jian Feng Xu ◽  
Ling Long ◽  
Hui Ren Hu ◽  
Yan Bo

A series of starch-based styrene acrylate emulsion (SAE) modified with water-resistance agent were prepared by introducing rosin, organosilane(γ-amino propyl triethoxysilane (KH550), γ-(2,3-epoxypropoxy)-propytrimethoxysilane(KH560) and γ-methacryloxypropyl trimethoxy silane (KH570) as the functional monomer, respectively. In the reaction, starch(CS-8), which is low relative viscosity, was used as the emulsifier and dispersant. The optimum conditions of the reaction had especially been studied by orthogonal experiment and then the emulsion properties and surface sizing behaviors of the SAE modified with different kinds of water-resistance agents were compared. Finally, the products which had excellent properties were characterized and the sizing performance was investigated. The results showed that the excellent performance and good sizing effects of styrene acrylate emulsion (SAE) were achieved when the reaction conditions were as follows: rosin was selected as the water resistance-agent with the dosage of 2%(wt), the reaction temperature was 88°C, the mass ratio of St and BA was 9:11, the stirring speed was 130 round per minute and the dosage of initiator (APS), cationic monomer (DM) and CS-8 was 0.5%, 2%(wt) and 7%, respectively. The water-resistance properties of starch-based SAE were significantly improved by using rosin as the water-resistance agent and the surface sizing performance were better than that of the commercial AKD.


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