scholarly journals Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient StrainBacillus subtilisUJS0717

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Zhuan Wei ◽  
Jue Zhou ◽  
WenJing Sun ◽  
FengJie Cui ◽  
QinHua Xu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Corn Steep Liquor ◽  
Culture Conditions ◽  
Medium Composition ◽  
Production Performance ◽  
Raw Material ◽  
Initial Ph ◽  
Fermentation Parameters ◽  
Inoculum Volume ◽  
Starch Hydrolysate

D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design. The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3. These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.

scholarly journals Improvement of lipase production from Geotrichum sp. in shaken flasks

2012 ◽  
Vol 18 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Resende Maldonadoa ◽  
Luiz Pancieraa ◽  
Alves Macedob ◽  
Marcio Mazuttic ◽  
Francisco Maugeria ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Lipase Activity ◽  
Corn Steep Liquor ◽  
Lipase Production ◽  
Inoculum Size ◽  
Inoculum Age ◽  
Initial Ph ◽  
Oil Concentration ◽  
Inoculum Volume ◽  
Steep Liquor

This work is focused on the study of different variables on inoculum build-up aiming to improve the lipase production by Geotrichum sp. by means a sequential strategy of experimental design. The effects of inoculum size, corn steep liquor concentration, volume of inoculum, pH of medium, age of inoculum and soybean oil concentration on lipase activity were assessed by means of two factorial experimental designs. A maximum lipase activity of 35.20?0.8 U/mL was obtained with a inoculum composed of one circular area of 0.78cm2 containing spores, 50 mL of inoculum volume medium, 12 hours of inoculum age, 15% w/v of corn steep liquor concentration, 1.0%w/v of soybean oil concentration and initial pH 5.0 at 30?C and 150 rpm in flasks. This work showed that an enhancement of lipase activity can be obtained using a sequential statistical factorial approach to define the variables for inoculum build-up.

Flocculating Activity Optimization of Lactobacillus paracasei subsp.paracasei L1 in Corn Liquid

2013 ◽  
Vol 850-851 ◽  
pp. 1172-1175
Author(s):  
Xiao Na Li ◽  
Xin Hua Li ◽  
Li Li Zhang ◽  
Jing Mu
Keyword(s):  
Culture Medium ◽  
Corn Starch ◽  
Culture Conditions ◽  
Lactobacillus Paracasei ◽  
Analysis Method ◽  
Medium Ph ◽  
Inoculum Concentration ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Surface Analysis Method

In order to improve the production process of corn starch and accelerate the separation of corn starch, flocculating activity of lactobacillus paracasei subsp.paracasei L1 isolated from natural fermentation of sweet potato acid liquor in corn liquid was optimized. The main influencing factors were determined by Plackett-Burman experimental design and the flocculating activity was optimized by response surface analysis method. The optimum culture conditions was: inoculum concentration 18%, lactose addition 0.2%, corn steeping time 0h, culture temperature 30°C, initial pH of the culture medium pH 6.5, culture time 36h, yeast extract addition 1%,concentration of corn thick liquid 1:3(w/v).The theoretical value of the flocculating activity was 0.1906 mg/ml and the verified value was 0.1854 mg/ml.

Optimization of Culture Conditions for Biosynthesis of Lipopeptide by Bacillus Subtilis with Starch

2013 ◽  
Vol 291-294 ◽  
pp. 225-229 ◽  
Author(s):  
Zong Ming Zheng ◽  
Ti Peng Wang ◽  
Qin Wu
Keyword(s):  
Bacillus Subtilis ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Ph Optimum ◽  
Linear Effect ◽  
Initial Ph ◽  
Culture Cycle ◽  
Optimization Of Culture Conditions ◽  
Fermentation Parameters ◽  
Main Effects

Optimization of culture conditions for lipopeptide production of lipopeptide by Bacillus subtilis NEL-01 was carried out in shaker flask batch fermentations using composite central design of response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum lipopeptide production at optimum levels for culture temperature, initial pH and culture cycle. Culture temperature and culture cycle showed the significant linear main effects, while pH had no significant linear effect. The production was also significantly affected by quadratic effect of culture temperature and initial pH. Optimum fermentation parameters were predicted at temperature, initial pH, and culture cycle of 34.81 °C, 7.33g/l, 49.26 h, respectively. The prediction lipopeptide yield was 1879.56 mg/l. The subsequent experiments confirmed the prediction.

scholarly journals Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Rayda Siala ◽  
Fakher Frikha ◽  
Samiha Mhamdi ◽  
Moncef Nasri ◽  
Alya Sellami Kamoun
Keyword(s):  
Culture Conditions ◽  
Medium Composition ◽  
Acid Protease ◽  
Protease Production ◽  
Statistical Experimental Design ◽  
Initial Ph ◽  
Burman Design ◽  
The Cost ◽  
Optimized Conditions ◽  
Plackett Burman Design

Medium composition and culture conditions for the acid protease production byAspergillus nigerI1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) withR2=0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO41.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2(7H2O) 0.4; ZnSO40.1; Na2HPO41.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.

Enhanced Activity of Intracellular Agarase from a Novel Marine Strain Agarivorans gilvus WH0801

2012 ◽  
Vol 554-556 ◽  
pp. 1227-1232 ◽  
Author(s):  
Wen Xue Gu ◽  
Yu Lin Chen ◽  
Hui Na Niu ◽  
Xiao Lu ◽  
Xiang Zhao Mao ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Fold Increase ◽  
Culture Conditions ◽  
Medium Composition ◽  
Optimization Strategy ◽  
Agarase Activity ◽  
Seed Age ◽  
Initial Ph ◽  
Agar Degradation ◽  
Marine Strain

A marine bacterium strain Agarivorans gilvus WH0801 with the efficient agar degradation ability isolated from fresh seaweed samples of Weihai coast was found to be potential in producing agarase. We studied on the optimal medium composition and culture conditions of Agarivorans gilvus WH0801 by statistical methods in shake flasks. First, several more important factors influencing agarase activity were selected by Plackett-Burman design. They are agar concentration, yeast extract concentration and seed age. Then the optimum levels of these three variables were further determined using Box-Behnken design. The highest agarase activity is obtained in the medium consisting of 2.49 g L-1 agar and 0.88 g L-1 yeast extract when the seed age is 25.64 h. The levels of other factors are 1 g L-1 peptone, 0.01 g L-1 ironic citrate at initial pH 7.0 and 28 °C. The whole optimization strategy results in the activity of agarase reaches 1.158 U mL-1, which is about 6.2-fold increase compares with the control.

scholarly journals PSII-21 Effects of barley starch proportion in diets on nutrient digestion, rumen fermentation parameters and rumen microorganisms of fattening Hu sheep

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 404-406
Author(s):  
Xiaowen Ma ◽  
Fadi Li ◽  
Fei Li
Keyword(s):  
Corn Starch ◽  
Rumen Fermentation ◽  
Shannon Index ◽  
Nutrient Digestibility ◽  
Production Performance ◽  
Rumen Microorganisms ◽  
Nutrient Digestion ◽  
Fermentation Parameters ◽  
The One ◽  
Hu Sheep

Abstract The purpose of this experiment was to study the effects of fattening Hu sheep diet with barley starch instead of corn starch in different proportions on the production performance, nutrient digestion, rumen fermentation parameters and rumen microbial of fattening Hu sheep. In this experiment, 72 Hu lambs (average BW:29.70 ± 1.70 kg) were selected and randomly divided into 4 treatments according to single factor block experiment. Each treatment included 18 replicates(n=18) and each sheep was raised in individual pen. Barely starch (BS) and corn starch (CS) were the starch source of the diets. Four treatments are BS-0 group(0%BS+100%CS), BS-33 group(33%BS+66%CS), BS-66 group(66%BS+33%CS), BS-100 group(100%BS+0%CS). The experiment lasted for 79 days with 7 days pre-feeding period and 63 days formal period(n=18), the last 6 days and 3 days were assigned to the digestibility(n=6) and sampled(n=10) after slaughter respectively. All dates were analyzed using the one-way ANOVE of SPSS 25.0. The results showed that the DM, OM, NDF and ADF digestibility of BS-0 group were higher than BS-100 group (P<0.05). the DM, OM and NDF digestibility of BS-66 group were higher than BS-100 group. Total VFA concentration of BS-66 and BS-100 is significantly higher than BS-33 and BS-0 (P<0.05). the propionate concentration of BS-33 is lower than BS-66 (P<0.05). The ruminal pH and NH3-N concentration of BS-33 are higher than BS-66 (P<0.05).BS-0 group has a higher ACE Chao1 and Shannon index than BS-100. At the phylum level, the abundance of Firmicutes increased and the abundance of Proteobacteria decreased in BS-100(P<0.05). At the genus level, the abundance of Ruminobacter in BS-100 is higher than other groups (P<0.05). These results indicated that the feeding efficiency of corn starch has positive affect than barley starch. 66% barley starch substitutes corn starch can improve nutrient digestibility and be good for rumen health.

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

scholarly journals Synthesis of the Microbial Polysaccharide Gellan from Dairy and Plant-Based Processing Coproducts

2021 ◽  
Vol 2 (2) ◽  
pp. 234-244
Author(s):  
Thomas P. West
Keyword(s):  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Culture Conditions ◽  
Water Soluble ◽  
Production Costs ◽  
Microbial Polysaccharide ◽  
Steep Liquor ◽  
The Cost ◽  
Condensed Distillers Solubles

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.

The effect of asynchronous diets on the function of the rumen in the lactating dairy cow

1998 ◽  
Vol 1998 ◽  
pp. 19-19
Author(s):  
A.R. Henderson ◽  
P.C. Garnsworthy ◽  
J.R. Newbold ◽  
P.J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Production Performance ◽  
Early Lactation ◽  
Microbial Protein ◽  
Nutrient Flows ◽  
Lactating Dairy Cows ◽  
Overall Efficiency ◽  
Fermentation Parameters

Sinclair et al. (1993) found that a diet formulated to be synchronous with regard to hourly release of nitrogen and energy increased microbial protein synthesis by 14% in sheep. Dairy cows in early lactation experience a shortfall of energy and protein, with available protein determining the overall efficiency of metabolism (MacRae and Lobley, 1986) and subsequent milk production. It is therefore necessary to maximise microbial protein yield during this period. In this study diets were designed for lactating dairy cows to contain the same feed ingredients, but to release nitrogen and energy in the rumen at different times. Rumen fermentation parameters, nutrient flows to the small intestine and production performance were investigated.

scholarly journals Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽  
2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Abdullah Abdullah ◽  
H. B. Mat
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Pineapple Waste ◽  
Fermentation Liquid ◽  
Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

Sign in / Sign up

Export Citation Format

Share Document