Using Chitosan Besides Nano Hydroxyapatite and Fluorohydroxyapatite Boost Dental Pulp Stem Cell Proliferation

2019 ◽  
Vol 42 ◽  
pp. 39-50
Author(s):  
Farbod Tondnevis ◽  
Mohammadali Ketabi ◽  
Reza Fekrazad ◽  
Ali Sadeghi ◽  
Mohamad Mahdi Abolhasani
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Contact Angle ◽  
Stem Cell ◽  
Dental Pulp ◽  
Freeze Drying ◽  
Cell Attachment ◽  
Dental Tissue ◽  
Dental Pulp Stem Cell ◽  
Dental Tissue Engineering

The dental tissue scaffold must provide a favorable surface for dental pulp stem cell attachment and proliferation. Employing nanohydroxyapatite (HA) and nanofluorohydroxyapatite (FHA) beside synthetic and organic polymer in favor of scaffolds would be used in bone and dental tissue engineering. In this research, nanoHA and FHA/chitosan scaffolds were synthesized by freeze-drying technique. Surface morphology, chemical composition and hydrophilicity have a great impact on initial cell attachment which will further affect the cell viability and proliferation which evaluated by SEM, XRD and contact angle measurement. Bioactivity of scaffolds was investigated by immersion in simulated body fluid (SBF) and cell proliferation assay. In freeze-drying technique percentage usage of hydroxyapatite could be risen up to 40% and shown better macro-mechanical and physical properties and bioactivity. According to obtained results by adding chitosan, contact angle was decreased by %54 and %37 for polycaprolactone (PCL)/HA and PCL/FHA scaffolds. In addition, addition of chitosan causes significant increase in the cell proliferation for PCL/HA and PCL/FHA up to 81% and 164%, respectively. These results indicate that PCL/FHA/chitosan scaffold represent a big potential for dental tissue engineering.

Dental Tissue Engineering Research and Translational Approaches towards Clinical Application

2013 ◽  
pp. 279-312
Author(s):  
Athina Bakopoulou ◽  
Gabriele Leyhausen ◽  
Werner Geurtsen ◽  
Petros Koidis
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Clinical Application ◽  
Exciting Field ◽  
Dental Tissue ◽  
Engineering Research ◽  
Biomimetic Scaffold ◽  
Research Findings ◽  
Dental Tissue Engineering ◽  
State Of The Science

Stem cell-based dental tissue regeneration is a new and exciting field that has the potential to transform the way that we practice dentistry. It is, however, imperative its clinical application is supported by solid basic and translational research. In this way, the full extent of the potential risks involved in the use of these technologies will be understood, and the means to prevent them will be discovered. Therefore, the aim of this chapter is to analyze the state-of-the-science with regard to dental pulp stem cell research in dental tissue engineering, the new developments in biomimetic scaffold materials customized for dental tissue applications, and to give a prospectus with respect to translational approaches of these research findings towards clinical application.

Dental Tissue Engineering Research and Translational Approaches towards Clinical Application

2017 ◽  
pp. 186-220
Author(s):  
Athina Bakopoulou ◽  
Gabriele Leyhausen ◽  
Werner Geurtsen ◽  
Petros Koidis
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Clinical Application ◽  
Exciting Field ◽  
Dental Tissue ◽  
Engineering Research ◽  
Biomimetic Scaffold ◽  
Research Findings ◽  
Dental Tissue Engineering ◽  
State Of The Science

Stem cell-based dental tissue regeneration is a new and exciting field that has the potential to transform the way that we practice dentistry. It is, however, imperative its clinical application is supported by solid basic and translational research. In this way, the full extent of the potential risks involved in the use of these technologies will be understood, and the means to prevent them will be discovered. Therefore, the aim of this chapter is to analyze the state-of-the-science with regard to dental pulp stem cell research in dental tissue engineering, the new developments in biomimetic scaffold materials customized for dental tissue applications, and to give a prospectus with respect to translational approaches of these research findings towards clinical application.

Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

2011 ◽  
Vol 11 ◽  
pp. 13-20 ◽  
Author(s):  
Tran Le Bao Ha ◽  
Doan Nguyen Vu ◽  
To Minh Quan ◽  
Ngoc Phan Kim ◽  
Hung Hoang Tu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Dental Tissue ◽  
Dentin Surface ◽  
Human Dental Pulp ◽  
Dental Tissue Engineering ◽  
High Level

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

scholarly journals Effects of TAZ on human dental pulp stem cell proliferation and migration

2017 ◽  
Vol 15 (6) ◽  
pp. 4326-4332 ◽  
Author(s):  
Songbo Tian ◽  
Xiaochao Tian ◽  
Yanping Liu ◽  
Fusheng Dong ◽  
Jie Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Dental Pulp ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Migration ◽  
Human Dental Pulp ◽  
Dental Pulp Stem Cell ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Stem Cell and Biomaterials Research in Dental Tissue Engineering and Regeneration

2012 ◽  
Vol 56 (3) ◽  
pp. 495-520 ◽  
Author(s):  
Orapin V. Horst ◽  
Miquella G. Chavez ◽  
Andrew H. Jheon ◽  
Tejal Desai ◽  
Ophir D. Klein
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Dental Tissue ◽  
Dental Tissue Engineering
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