Investigation of Bone Formation using Non-Crystalline Calcium Phosphate Glass in Beagle Dogs

The purpose of this study was to compare the bone regenerative effect of calcium phosphate glass according to the particle size in vivo. We prepared two different sizes, that is 400 μm and 40 μm, of calcium phosphate glass powder using the system CaO-CaF2-P2O5-MgO-ZnO. Critical-sized calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 3 groups of 20 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As controls, the defect was left empty. The rats were sacrificed 2 or 8 weeks after postsurgery, and the results were evaluated using histological as well as histomorphometrical studies. The particle size of the calcium phosphate was crucial; 400 μm particles promoted new bone formation, while 40 μm particles inhibited it because of severe inflammation.

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Effect of calcium phosphate glass on bone formation in calvarial defects of Sprague-Dawley rats

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-006-9839-9 ◽

2006 ◽

Vol 17 (9) ◽

pp. 807-813 ◽

Cited By ~ 8

Author(s):

Hyun-Ju Moon ◽

Kyoung-Nam Kim ◽

Kwang-Mahn Kim ◽

Seong-Ho Choi ◽

Chong-Kwan Kim ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Phosphate Glass ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Calvarial Defects

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Bone formation in calvarial defects of Sprague-Dawley rats by transplantation of calcium phosphate glass

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.30408 ◽

2005 ◽

Vol 74A (3) ◽

pp. 497-502 ◽

Cited By ~ 14

Author(s):

Hyun-Ju Moon ◽

Kyoung-Nam Kim ◽

Kwang-Mahn Kim ◽

Seong-Ho Choi ◽

Chong-Kwan Kim ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Phosphate Glass ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Calvarial Defects

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Amorphous Calcium Polyphosphate Bone Regenerative Materials Based on Calcium Phosphate Glass

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.209 ◽

2008 ◽

Vol 396-398 ◽

pp. 209-212 ◽

Cited By ~ 8

Author(s):

Byung Hyun Lee ◽

Min Chul Kim ◽

Seong Ho Choi ◽

Yong Keun Lee

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Amorphous Phase ◽

Phosphate Glass ◽

Control Group ◽

New Bone Formation ◽

Inorganic Polyphosphates ◽

Calcium Polyphosphate ◽

Naoh Solution ◽

New Material

We developed new calcium phosphate bone substitute material, amorphous calcium polyphosphate. The new material is synthesized by a cement-like slif-setting reaction with calcium phosphate glass, basic materials and water. In this study, we prepared with CPG, Na2CO3 and NaOH solution. When they are mixed together, amorphous phase was precipitated. The precipitated amorphous phase consisted of polyphosphate chains condensed with Na ions released from Na2CO3 and NaOH. When the amorphous calcium polyphosphate dissolves, inorganic polyphosphates are released into the medium. The inorganic polyphosphates as the dissolution product inducted the calcification of the osteoblast cells. Therefore, in animal test, the new bone formation in rat calvarial defects treated with the new material was significantly higher than sham-surgery control group, especially in the initial stage. The amorphous calcium polyphosphate was biocompatible and bioresorbable and promoted the new bone formation.

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The effects of hydroxyapatite/calcium phosphate glass scaffold and its surface modification with bovine serum albumin on 1-wall intrabony defects of beagle dogs: a preliminary study

Biomedical Materials ◽

10.1088/1748-6041/3/4/044113 ◽

2008 ◽

Vol 3 (4) ◽

pp. 044113 ◽

Cited By ~ 2

Author(s):

Yoo-Jung Um ◽

Ui-Won Jung ◽

Gyung-Joon Chae ◽

Chang-Sung Kim ◽

Yong-Keun Lee ◽

...

Keyword(s):

Surface Modification ◽

Calcium Phosphate ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Phosphate Glass ◽

Bovine Serum ◽

Beagle Dogs ◽

Intrabony Defects ◽

Preliminary Study

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Bone Formation of Intrabony Defects in Beagle Dogs Using Calcium Phosphate Glass and Glass-Ceramics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.284-286.957 ◽

2005 ◽

Vol 284-286 ◽

pp. 957-960 ◽

Cited By ~ 1

Author(s):

Seong Ho Choi ◽

D.H. Baik ◽

C.S. Kim ◽

Chong Kwan Kim ◽

Kyoung Nam Kim ◽

...

Keyword(s):

Calcium Phosphate ◽

Phosphate Glass ◽

Alveolar Bone ◽

Root Resorption ◽

Glass Ceramics ◽

Beagle Dogs ◽

Sprague Dawley ◽

Intrabony Defects ◽

Prepared Glass ◽

Significant Difference

The purpose of this study was to compare the bone regenerative effect of calcium phosphate glass and glass-ceramics in the system of CaO-CaF2-P2O5-MgO-ZnO, which is already reported that promoted the bone-like tissue formation in vitro and formed new bone in Sprague-Dawley rats. We prepared calcium phosphate glass and glass-ceramics with Ca/P ratio of 0.6 using the system CaO-CaF2-P2O5-MgO-ZnO and subsequently milled to 400 µm. 4×4 mm 1-wall intrabony defects of six beagle dogs were surgically created and used in this study. 8 weeks after transplantation of the prepared calcium phosphate glass and glass-ceramics in the experimental group 1 and 2, respectively, the animals were sacrificed. No root resorption or ankylosis were observed in all groups. It can be examined that the prepared glass-ceramics were more effective in alveolar bone regeneration with statistical signigicant difference. In cementum regeneration, both the prepared glass and glass-ceramics showed significantly different effect. However, there were no statistical significant difference neither in glass nor glass-ceramics in the amounts of junctional epithelium migration and connective tissue adhesion. Further study is required to control the flowability as well as reduce the absorption rate in vivo.

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