Evaluation of Gamma Irradiated Human Bone in Nude Rats

2005 ◽  
Vol 288-289 ◽  
pp. 269-272
Author(s):  
Y. Yu ◽  
Jin Biao Chen ◽  
J.L. Yang ◽  
D.A.F. Morgan ◽  
W.R. Walsh
Keyword(s):  
Bone Formation ◽  
Gamma Irradiation ◽  
Soft Tissues ◽  
Bone Grafts ◽  
Human Bone ◽  
New Bone Formation ◽  
Nude Rats ◽  
Defect Area ◽  
Post Operation ◽  
Gamma Irradiated

Deep-frozen morselized human bone grafts showed osteoconductivity and osteoinductivity when implanted into tibial window defects of nude rats. The osteoconductivity was assessed by measuring the total area of newly formed bone bridged by the implanted bone grafts in the entire defect area. The osteoinductivity was evidenced by the presence of active osteoblast-like cells and new bone formation around the implanted bone grafts, which were surrounded by soft tissues distant from the host cortex. Gamma irradiation at the doses of 15 or 25 kGray reduced the osteoconductivity (ANOVA and LSD tests, p<0.05) at 3 weeks post operation. The 25 kGray group had a significantly lower level of new bone formation compared with the 0 and 15 kGray groups. The evidences of osteoinductivity were only noted in the 0 and 15 kGray groups. Our data indicate that 25 kGray gamma irradiation reduces the osteoconductive and osteoinductive properties of the morselized human bone graft.

Biological Response to Wollastonite Doped α-Tricalcium Phosphate Implants in Hard and Soft Tissues in Rats

2008 ◽  
Vol 396-398 ◽  
pp. 7-10 ◽  
Author(s):  
Ana Maria Minarelli Gaspar ◽  
Sybele Saska ◽  
R. García Carrodeguas ◽  
A.H. De Aza ◽  
P. Pena ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Rattus Norvegicus ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
New Bone Formation ◽  
Rat Bone

The biological response following subcutaneous and bone implantation of β-wollastonite(β-W)-doped α-tricalcium phosphate bioceramics in rats was evaluated. Tested materials were: tricalcium phosphate (TCP), consisting of a mixture of α- and β-polymorphs; TCP doped with 5 wt. % of β-W (TCP5W), composed of α-TCP as only crystalline phase; and TCP doped with 15 wt. % of β-W (TCP15), containing crystalline α-TCP and β-W. Cylinders of 2x1 mm were implanted in tibiae and backs of adult male Rattus norvegicus, Holtzman rats. After 7, 30 and 120 days, animals were sacrificed and the tissue blocks containing the implants were excised, fixed and processed for histological examination. TCP, TCP5W and TCP15W implants were biocompatible but neither bioactive nor biodegradable in rat subcutaneous tissue. They were not osteoinductive in connective tissue either. However, in rat bone tissue β-W-doped α-TCP implants (TCP5W and TCP15W) were bioactive, biodegradable and osteoconductive. The rates of biodegradation and new bone formation observed for TCP5W and TCP15W implants in rat bone tissue were greater than for non-doped TCP.

scholarly journals The use of solvent-preserved human and bovine cancellous bone blocks for lateral defect augmentation - an experimental controlled study in vivo

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Lara Schorn ◽  
Tim Fienitz ◽  
Kathrin Berndsen ◽  
Norbert R. Kübler ◽  
Henrik Holtmann ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Substitute ◽  
Bone Matrix ◽  
Human Bone ◽  
Controlled Study ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Substitute Material

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

Induction of new-bone formation in the host bed by human bone-tumor transplants in athymic nude mice.

1979 ◽  
Vol 61 (8) ◽  
pp. 1207-1216 ◽  
Author(s):  
M R Urist ◽  
T T Grant ◽  
T S Lindholm ◽  
J M Mirra ◽  
H Hirano ◽  
...  
Keyword(s):  
Bone Formation ◽  
Nude Mice ◽  
Bone Tumor ◽  
Human Bone ◽  
New Bone Formation ◽  
Athymic Nude Mice

Quantitative Analysis of New Bone Formation in Various Bone Grafts by In - Vivo Tetracycline Labeling

1985 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Sang Cheol Seong ◽  
Young Min Kim ◽  
Han Koo Lee ◽  
In Ho Choi ◽  
Moon Sang Chung ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation ◽  
Tetracycline Labeling

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

Comparison of Bone Substitutes in a Tibia Defect Model in Wistar-Rats

2011 ◽  
Vol 493-494 ◽  
pp. 732-738 ◽  
Author(s):  
Cornelia Ganz ◽  
W. Xu ◽  
G. Holzhüter ◽  
W. Götz ◽  
B. Vollmar ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Substitutes ◽  
Porous Matrix ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Defect Model ◽  
Inflammatory Reactions ◽  
Nanocrystalline Hydroxyapatite ◽  
Defect Area ◽  
Tibia Defect

Various bone graft substitutes were used in clinical practise in the treatment of bone defects after trauma or osteoporosis. Many synthetic biomaterials were developed in recent years primarily based on hydroxyapatite (HA). NanoBone® is a nanocrystalline hydroxyapatite (HA) embedded in a porous matrix of silica (SiO2). The ratio of HA:SiO2 varied between 76:24 (wt%; NanoBone®) and 61:39 (wt%; Nanobone® S). The two bone substitutes NB and NB S and a natural bovine bone substitute Bio-Oss® (BO) were evaluated by means of implantation in the tibia of the rat. The aim of this study was to analyze the remodelling process and to measure new bone formation and degradation after implantation of these biomaterials. A tibia defect model was used for all investigations with testing periods of 12, 21 and 84 days. (n=5 for each time point). The results showed, that all bone grafts were well accepted by the host tissue without inflammatory reactions. In comparison to the biomaterial BO, NanoBone® and NanoBone® S were quickly degraded, whereas autologous proteins were incorporated into nanopores. New bone formation was statistically higher in NanoBone® S compared to Bio-Oss® in defect area after 84 days implantation. The presence of osteoclasts in tissue sections were demonstrated by TRAP- and ED1-immunohistology.

Retracted: New bone formation in bone defects after melatonin and porcine bone grafts: experimental study in rabbits

2014 ◽  
Vol 26 (4) ◽  
pp. 399-406 ◽  
Author(s):  
José-Luis Calvo-Guirado ◽  
Gerardo Gómez-Moreno ◽  
José-Eduardo Maté-Sánchez ◽  
Laura López-Marí ◽  
Rafael Delgado-Ruiz ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Formation ◽  
Bone Grafts ◽  
Bone Defects ◽  
New Bone Formation
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