In Vivo Evaluation of Porous Hydroxyapatite/Collagen Composite as a Carrier of OP-1 in a Rabbit PLF Model

Transplantation surgeries of autologous bone require a second surgery with inherent risks. To avoid these risks, we developed a multi porous implant of hydroxyapatite/collagen composite with desirable biophysical properties (flexibility, elasticity and compression resistance) for use with OP-1 as a graft implant. In this study, we tested the efficacy of this multi porous implant as OP-1 carrier using rabbit posterolateral lumbar fusion model (PLF). PLFs were performed in the following 4 groups of 8 New Zealand white rabbits each: autograft, HAp/Col alone, HAp/Col plus 0.3 mg OP-1, and HAp/Col plus 1.2 mg OP-1. At 5 weeks, fusion masses were analyzed by radiographic and biomechanical tests. Implants consisting of HAp/Col plus OP-1 were more effective than autologous bone in promoting spinal fusion. Low dose and high dose OP-1 were equally effective.

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In vivo evaluation of bioresorbable polylactide implants for cervical graft containment in an ovine spinal fusion model

Neurosurgical FOCUS ◽

10.3171/foc.2004.16.3.6 ◽

2004 ◽

Vol 16 (3) ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

G. Bryan Cornwall ◽

Christopher P. Ames ◽

Neil R. Crawford ◽

Robert H. Chamberlain ◽

Anthony M. Rubino ◽

...

Keyword(s):

Anterior Cervical Discectomy ◽

Complete Fusion ◽

Fusion Model ◽

In Vivo Evaluation ◽

Cervical Discectomy ◽

Single Level ◽

Screw Breakage ◽

Time Periods ◽

Bioresorbable Polymer

Object An in vivo study was conducted in an ovine model to investigate the biomechanical changes after the animals underwent single-level anterior cervical discectomy followed by fusion in which autologous tricortical graft was used and implantation of cervical plates for which bioresorbable polymer screws and plates were used. The specific aims of the study were to evaluate whether implant failure or screw backout would occur over time and to measure the change in stiffness at the treated level at various postoperative time periods (3, 6, and 12 months). Methods A total of 58 x-ray films were evaluated over the 12-month survival period. No screw breakage or displacement was observed in any animal during the temporal radiographic analysis. Radiographically confirmed fusion appeared to be complete at all time periods longer than 6 months. The biomechanical testing demonstrated dramatic reductions in range of motion at the fusion level in the animals allowed to survive for 6 and 12 months, indicating complete fusion after 6 months. Conclusions The bioresorbable polymer cervical graft containment system appears to provide a safe and effective alternative for cervical fusion, and warrants further clinical evaluation for its use in single-level anterior cervical discectomy and fusion without postoperative orthosis.

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In vivo evaluation of porous hydroxyapatite ceramics including bone minerals using pig model

Materials Technology ◽

10.1080/10667857.2018.1495392 ◽

2018 ◽

Vol 33 (10) ◽

pp. 689-697 ◽

Cited By ~ 1

Author(s):

Tomohiro Yokota ◽

Kazuaki Nakano ◽

Masaki Nagaya ◽

Michiyo Honda ◽

Hiroshi Nagashima ◽

...

Keyword(s):

Pig Model ◽

In Vivo Evaluation ◽

Porous Hydroxyapatite ◽

Hydroxyapatite Ceramics ◽

Bone Minerals

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Standard Guide for in vivo Evaluation of Rabbit Lumbar Intertransverse Process Spinal Fusion Model

10.1520/f3207-17 ◽

2017 ◽

Author(s):

Keyword(s):

Spinal Fusion ◽

Fusion Model ◽

In Vivo Evaluation

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In Vivo Evaluation of Porous Hydroxyapatite/Poly D/L-Lactide Composite for Bone Substitute and Scaffold

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.284-286.769 ◽

2005 ◽

Vol 284-286 ◽

pp. 769-774

Author(s):

Shin Hasegawa ◽

Jiro Tamura ◽

Masashi Neo ◽

Koji Goto ◽

Yasuo Shikinami ◽

...

Keyword(s):

Bone Marrow ◽

Bone Formation ◽

Bone Remodeling ◽

Bone Substitute ◽

Femoral Condyle ◽

Marrow Cell ◽

In Vivo Evaluation ◽

Porous Hydroxyapatite ◽

Cell Scaffolds

We investigated the biocompatibility, osteoconductivity, and biodegradability of porous composite of Hydroxyapatite (HA) and Poly D/L-lactide (PDLLA). At 6weeks afterimplantation to rabbit femoral condyle, HA/PDLLA was covered with bone and contacted with bone directly. The amounts of newly formed bone in the pores had increased during the examined period. By 26weeks, bone remodeling of formed bone in the pores was seen and bone marrow tissue formation was seen in the pores of HA/PDLLA. Porous HA/PDLLA was resorbed much faster than porous HA as a control. Porous HA/PDLLA was resorbed constantly through the bone formation and bone remodeling but porous HA was hardly resorbed during the period. It might be one of the desirable materials for bone substitute. To evaluate for a scaffold, disc shaped blocks loaded with rat bone marrow cell were implanted in the subcutaneous pouch of the back of syngeneic rat. At 3weeks afterimplantation, newly bone formation in the pores was observed at ectopic site. It also suggested the availability of this material as cell scaffolds.

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A Porous Hydroxyapatite/Gelatin Nanocomposite Scaffold for Bone Tissue Repair: In Vitro and In Vivo Evaluation

Journal of Biomaterials Science Polymer Edition ◽

10.1163/156856211x617713 ◽

2012 ◽

Vol 23 (18) ◽

pp. 2353-2368 ◽

Cited By ~ 42

Author(s):

Mahmoud Azami ◽

Shima Tavakol ◽

Ali Samadikuchaksaraei ◽

Mehran Solati Hashjin ◽

Nafiseh Baheiraei ◽

...

Keyword(s):

Bone Tissue ◽

Tissue Repair ◽

In Vivo Evaluation ◽

Porous Hydroxyapatite ◽

Nanocomposite Scaffold

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In Vivo Evaluation of Jatropha curcas L (Euphorbiaceae) Leaves Acute and Subacute Toxicity in Mice

Journal of Scientific Research ◽

10.3329/jsr.v10i2.35267 ◽

2018 ◽

Vol 10 (2) ◽

pp. 187-193 ◽

Cited By ~ 3

Author(s):

S. Sawadogo ◽

S. D. Sanou ◽

A. P. Dabiré ◽

G. R. Belemtougri ◽

L. Sawadogo ◽

...

Keyword(s):

Aqueous Extract ◽

Jatropha Curcas ◽

Histological Study ◽

Significant Rise ◽

Toxicity Study ◽

High Dose ◽

In Vivo Evaluation ◽

Subacute Toxicity ◽

Hematological Analysis

Jatropha curcas is a medicinal plant whose dead leaves are particularly used in arterial hypertension and diabetes treatment. The purpose of this study is to conduct an acute and subacute toxicity study of Jatropha curcas leaves aqueous extract (JCLAE) using OECD method. For acute toxicity, dose limits of 2000 and 5000 mg/kg were used. In subacute toxicity study 4 batches were constituted including a control batch that received distilled water for 28 days and the other 3 batches, JCLAE doses of respectively 200, 400, 800 mg/kg for the same duration. The LD50 was determined and the hematological, biochemical and histological parameters were analyzed in mice. The LD50 is greater than 5000 mg/kg. HDL-C is the only biochemical parameter that has experienced significant rise variation. Hematological analysis showed a decrease in mean platelet volume and Platelets number. The histological study revealed cases of hepatic cellular apoptosis and kidneys tubular necrosis among animals treated with highest dose. JCLAE is less toxic than Jatropha curcas leaves aqueous extract and high-dose JCLAE also has a moderate toxic effect on thrombocyte line and a protective effect on cardiovascular system.

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Formulation, Optimization and In Vivo Evaluation of Fucoidan-Based Cream with Anti-Inflammatory Properties

Marine Drugs ◽

10.3390/md19110643 ◽

2021 ◽

Vol 19 (11) ◽

pp. 643

Author(s):

Ekaterina D. Obluchinskaya ◽

Olga N. Pozharitskaya ◽

Elena V. Flisyuk ◽

Alexander N. Shikov

Keyword(s):

Topical Application ◽

Colloidal Stability ◽

High Dose ◽

In Vivo Evaluation ◽

Release Studies ◽

Anti Inflammatory ◽

Kinetics Of ◽

Best Fit

Fucoidan is a polysaccharide found in brown alga with glorious potential for pharmacological activities, among which its anti-inflammatory properties have gained meaningful attention. Due to several advantages of formulations for topical application, this study aimed to develop and optimize a fucoidan-based cream formulation and to investigate its anti-inflammatory potential after topical application in vivo. Fucoidan from Fucus vesiculosus L. was used. The cream base consisting of olive oil and Kolliphor RH40 was optimized followed by in vitro agar diffusion and drug release studies. The fucoidan-based cream with 13% Kolliphor P 407, 1% Transcutol P, and 5% PEG400 showed good spreadability, washability, and colloidal stability, and it did not irritate the skin. The kinetics of fucoidan release from the optimized cream exhibited the best fit to the Korsmeyer–Peppas and Higuchi models with R2 > 0.99. Fucoidan release was controlled by drug diffusion and anomalous transport provided by the optimized cream base. The formulation was stable and provided high fucoidan release after storage for 1 year. Topical application of the fucoidan-based cream dose-dependently inhibited carrageenan-induced edema and ameliorated mechanical allodynia in rats. The efficacy of the fucoidan-based cream at a high dose was comparable with the efficacy of diclofenac gel. The fucoidan-based cream could be considered a promising anti-inflammatory formulation.

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High-Dose Benzodiazepines Positively Modulate GABAA Receptors via a Flumazenil-Insensitive Mechanism

International Journal of Molecular Sciences ◽

10.3390/ijms23010042 ◽

2021 ◽

Vol 23 (1) ◽

pp. 42

Author(s):

Na Wang ◽

Jingjing Lian ◽

Yanqing Cao ◽

Alai Muheyati ◽

Shanshan Yuan ◽

...

Keyword(s):

Gabaa Receptors ◽

High Dose ◽

High Concentration ◽

Concentration Effects ◽

In Vivo Evaluation ◽

Chemical Structures ◽

Loss Of Righting Reflex ◽

High Concentrations

Benzodiazepines (BZDs) produce versatile pharmacological actions through positive modulation of GABAA receptors (GABAARs). A previous study has demonstrated that high concentrations of diazepam potentiate GABA currents on the α1β2γ2 and α1β2 GABAARs in a flumazenil-insensitive manner. In this study, the high-concentration effects of BZDs and their sensitivity to flumazenil were determined on synaptic (α1β2γ2, α2β2γ2, α5β2γ2) and extra-synaptic (α4β2δ) GABAARs using the voltage-clamp electrophysiology technique. The in vivo evaluation of flumazenil-insensitive BZD effects was conducted in mice via the loss of righting reflex (LORR) test. Diazepam induced biphasic potentiation on the α1β2γ2, α2β2γ2 and α5β2γ2 GABAARs, but did not affect the α4β2δ receptor. In contrast to the nanomolar component of potentiation, the second potentiation elicited by micromolar diazepam was insensitive to flumazenil. Midazolam, clonazepam, and lorazepam at 200 µM exhibited similar flumazenil-insensitive effects on the α1β2γ2, α2β2γ2 and α5β2γ2 receptors, whereas the potentiation induced by 200 µM zolpidem or triazolam was abolished by flumazenil. Both the GABAAR antagonist pentylenetetrazol and Fa173, a proposed transmembrane site antagonist, abolished the potentiation induced by 200 µM diazepam. Consistent with the in vitro results, flumazenil antagonized the zolpidem-induced LORR, but not that induced by diazepam or midazolam. Pentylenetetrazol and Fa173 antagonized the diazepam-induced LORR. These findings support the existence of non-classical BZD binding sites on certain GABAAR subtypes and indicate that the flumazenil-insensitive effects depend on the chemical structures of BZD ligands.

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