The Effects of Collagen Membrane Coated with PLGA on Bone Regeneration in Rat Calvarial Defects

2007 ◽  
Vol 342-343 ◽  
pp. 357-360
Author(s):  
Kun Young Song ◽  
Yoo Jung Um ◽  
Ui Won Jung ◽  
Yong Keun Lee ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Collagen Membrane ◽  
Histomorphometric Analysis ◽  
Local Bone ◽  
Calvarial Defects ◽  
Formation Behavior ◽  
Surgical Implantation ◽  
Collagen Membranes ◽  
Rat Calvarial Defect

The purpose of this study was to evaluate the effects of collagen membrane coated with PLGA on bone regeneration in rat calvarial defect. Five groups of 10 animals each received either collagen membrane coated with 0.5%, 1%, 3% concentration of PLGA, collagen membrane only or surgical control. Each group of animals was healed into 2 healing periods of 2(5 animals) and 8(5 animals)weeks and histologic and histomorphometric analysis were done. The results of the following study revealed that surgical implantation of collagen membranes coated with PLGA enhanced local bone formation at both 2 and 8 weeks independent of different PLGA concentrations. In conclusion, collagen membrane coated with PLGA shows a significant bone formation behavior irrespective of their concentration.

Guiding Bone Regeneration with a Novel Biodegradable Polymeric Membrane and Bioceramic Bone Grafts around Dental Implants

2007 ◽  
Vol 330-332 ◽  
pp. 1417-1420
Author(s):  
L. Li ◽  
C.Y. Bao ◽  
Guo Min Ou ◽  
W.C. Chen ◽  
X.J. Zhang ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Dental Implants ◽  
Polymeric Membrane ◽  
Collagen Membrane ◽  
Graft Material ◽  
The Novel ◽  
Histomorphometric Analysis ◽  
Mongrel Dog ◽  
Collagen Membranes

The aim of this study is to evaluate treatment effects of novel GBR membranes (PDLLA membranes) applied to bony defects around dental implants on new bone regeneration alone or in combination with bioceramic bone graft (BIO-OSS®) on the mongrel dog model in comparison with collagen membrane (BIO-GIDE®) and therefore to assess the clinical values of the novel GBR membranes and bioceramic bone graft material in dental implantology. 6 implants were placed in bilateral mandibular of each mongrel dog after preparation of mid- crestal defects. The defects on one side were covered with PDLLA membranes alone or with bone graft, while the contra-lateral sides received collagen membranes alone or with bone graft. These animals were sacrificed at 2, 4, 8,12 weeks post the GBR-operation. And gross sample examination, standardized radiographs, 99mTc-MDP SPECT and histomorphometric analysis were taken. All examinations showed similar amounts of newly formed bone beneath both types of barriers. The PDLLA or collagen membrane with bone graft groups showed better results than the membrane used alone groups. The results indicated that PDLLA membranes especially with bioceramic bone graft presented an improved response in dealing with bony defects around implants.

scholarly journals Evaluation of Open Hollow Hydroxyapatite Microsphere on Bone Regeneration in Rat Calvarial Defects

2019 ◽  
Author(s):  
Youqu Shen ◽  
Mohamed Rahaman ◽  
Yongxian Liu ◽  
Yue-Wern Huang
Keyword(s):  
Surface Roughness ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Convex Surface ◽  
Total Surface Area ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Calvarial Defects ◽  
The Difference ◽  
Rat Calvarial Defect

AbstractHollow hydroxyapatite (HA) microspheres showed the ability to facilitate bone regeneration in rats with non-healing calvarial defects. However, new bone formation in the rat calvarial defect implanted with the closed HA microspheres was limited. The objective of this work is to evaluate size-, time, and structure-dependent bone regeneration between open and closed HA microspheres in an osseous model. Open HA microspheres were obtained by sectioning closed HA microspheres. The open HA microsphere had dense convex surface and rough and porous concave surface. For both size ranges (ϕ106-150 μm vs. ϕ212-250 μm), the open HA microsphere were more effective in facilitating bone regeneration than the closed HA microspheres in rat calvarial defects. Bone regeneration in the open HA microspheres (49 ± 7% for ϕ106-150 μm; 40 ± 8% for ϕ212-250 μm) were higher than the closed HA microsphere (26 ± 8% for ϕ106-150 μm; 30 ± 9% for ϕ212-250 μm) at 12 weeks. Furthermore, the open HA microspheres of smaller size showed a significant increase in bone regeneration than the open HA microspheres of larger size at both 6 weeks and 12 weeks. The difference in bone regeneration between these microspheres could be due to their differences in microstructures, namely curvature, concavity, porosity, surface roughness, and total surface area available for cells to attached to.

scholarly journals Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2435 ◽  
Author(s):  
So-Yeun Kim ◽  
Eun-Bin Bae ◽  
Jae-Woong Huh ◽  
Jong-Ju Ahn ◽  
Hyun-Young Bae ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Graft Material ◽  
Implant Placement ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

scholarly journals Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs

Materials ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 4599
Author(s):  
Jong-Ju Ahn ◽  
Hyung-Joon Kim ◽  
Eun-Bin Bae ◽  
Won-Tak Cho ◽  
YunJeong Choi ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Physical Properties ◽  
Bone Regeneration ◽  
Enzymatic Degradation ◽  
Test Group ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Collagen Membrane ◽  
Micro Computed Tomography ◽  
Collagen Membranes

The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups (p > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups (p > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.

scholarly journals Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sahar Ansari ◽  
Marcelo O. Freire ◽  
Eun-Kyoung Pang ◽  
Alaa I. Abdelhamid ◽  
Mohammad Almohaimeed ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
De Novo ◽  
Isotype Control ◽  
Different Types ◽  
Calvarial Defects

Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the efficacy of immobilization of a specific murine anti-BMP-2 mAb on three different types of biomaterials and to evaluate their suitability as scaffolds for AMOR. Anti-BMP-2 mAb or isotype control mAb was immobilized on titanium (Ti) microbeads, alginate hydrogel, and ACS. The treated biomaterials were surgically implanted in rat critical-sized calvarial defects. After 8 weeks,de novobone formation was assessed using micro-CT and histomorphometric analyses. Results showedde novobone regeneration with all three scaffolds with immobilized anti-BMP-2 mAb, but not isotype control mAb. Ti microbeads showed the highest volume of bone regeneration, followed by ACS. Alginate showed the lowest volume of bone. Localization of BMP-2, -4, and -7 antigens was detected on all 3 scaffolds with immobilized anti-BMP-2 mAb implanted in calvarial defects. Altogether, these data suggested a potential mechanism for bone regeneration through entrapment of endogenous BMP-2, -4, and -7 proteins leading to bone formation using different types of scaffoldsviaAMOR.

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