Porous Hydroxyapatite Scaffolds by Polymer Sponge Method

2008 ◽  
Vol 396-398 ◽  
pp. 703-706 ◽  
Author(s):  
A.C.B.M. Fook ◽  
A.H. Aparecida ◽  
Thiago Bizerra Fideles ◽  
R.C. Costa ◽  
Marcus Vinícius Lia Fook
Keyword(s):  
Chemical Composition ◽  
Bone Regeneration ◽  
Bone Substitutes ◽  
High Porosity ◽  
Porous Hydroxyapatite ◽  
Pore Sizes ◽  
Hydroxyapatite Scaffold

This study aimed to develop porous hydroxyapatite scaffold for bone regeneration using the replica of the polymeric sponge technique. Polyurethane sponges were used with varying densities to obtain the scaffolds. The results indicate the porous HA scaffolds developed in this study as potential materials for application as bone substitutes to have high porosity (> 70%), chemical composition, interconnectivity and pore sizes appropriate to the bone regeneration.

Simvastatin Loaded Nano Hydroxyapatite in Bone Regeneration: A Study in the Rabbit Femoral Condyle

2019 ◽  
Vol 16 (6) ◽  
pp. 530-537 ◽  
Author(s):  
Elna Paul Chalisserry ◽  
Seung Yun Nam ◽  
Sukumaran Anil
Keyword(s):  
Bone Regeneration ◽  
Femoral Condyle ◽  
Bone Substitutes ◽  
Control Site ◽  
Bone Volume ◽  
Bone Morphogenetic Protein 2 ◽  
Micro Ct ◽  
Mineral Density ◽  
Hydroxyapatite Ceramics ◽  
Hydroxyapatite Scaffold

Background: Enhancement of the bone regenerative capacity of the bone substitutes could be achieved by incorporating bioactive agents such as proteins, and different drugs. Simvastatin, an inhibitor of cholesterol synthesis, stimulates bone formation by enhancing the expression of Bone Morphogenetic Protein-2 (BMP-2) in osteoblasts. Objective: The objective of the study is to evaluate bone regeneration following simvastatin loaded nano-hydroxyapatite scaffold in the bone defect created on the femoral condyle of rabbits. Methods: Twelve adult, New Zealand white rabbits were used in the study. Twenty-four defects of size 5x8 mm were created on the lateral aspect of the femoral condyle. The defects were filled with either Nano-Hydroxyapatite (nHA) particles alone or nHA with Simvastatin (SIM). The condyles were retrieved after 8 weeks and analyzed using micro CT and histology. Results: The Bone Mineral Density (BMD) was significantly higher for the defects filled with SIM loaded nHA compared to the nHA site. Micro CT showed a significantly higher bone volume in the defects filled with Simvastatin loaded site compared to the control site. Quantitative analysis of the histologic sections also showed significantly higher bone volume in the defects filled with SIM loaded nHA (57.2±4.8) compared to nHA alone (50.1±5.5). Conclusion: Based on the results, it can be concluded that local delivery of simvastatin enhanced the bone regeneration in rabbit femoral condyle. Simvastatin could be used as an activator to enhance bone regeneration in bone defects along with hydroxyapatite ceramics.

scholarly journals Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design

2016 ◽  
Vol 144 (5-6) ◽  
pp. 280-287 ◽  
Author(s):  
Nikola Sjerobabin ◽  
Bozana Colovic ◽  
Milan Petrovic ◽  
Dejan Markovic ◽  
Slavoljub Zivkovic ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Calcium Hydroxyapatite ◽  
Bone Substitutes ◽  
Sem Analysis ◽  
Optimal Topology ◽  
Cell Multiplication ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Polymer Foam ◽  
Hydroxyapatite Scaffold

Introduction. Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective. The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods. The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls? topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results. The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion. The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained.

scholarly journals Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration

Materials ◽  
2017 ◽  
Vol 10 (1) ◽  
pp. 33 ◽  
Author(s):  
Manabu Tanaka ◽  
Hisao Haniu ◽  
Takayuki Kamanaka ◽  
Takashi Takizawa ◽  
Atsushi Sobajima ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
In Vivo Evaluation ◽  
Porous Hydroxyapatite ◽  
Physico Chemical ◽  
Hydroxyapatite Scaffold

scholarly journals Osteogenic Properties of Novel Methylsulfonylmethane-Coated Hydroxyapatite Scaffold

2020 ◽  
Vol 21 (22) ◽  
pp. 8501
Author(s):  
Jeong-Hyun Ryu ◽  
Tae-Yun Kang ◽  
Hyunjung Shin ◽  
Kwang-Mahn Kim ◽  
Min-Ho Hong ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Regeneration ◽  
Porous Scaffold ◽  
Calcium Deposition ◽  
Burst Release ◽  
Alizarin Red ◽  
Porous Hydroxyapatite ◽  
Level Of Activity ◽  
Significant Difference ◽  
Hydroxyapatite Scaffold

Despite numerous advantages of using porous hydroxyapatite (HAp) scaffolds in bone regeneration, the material is limited in terms of osteoinduction. In this study, the porous scaffold made from nanosized HAp was coated with different concentrations of osteoinductive aqueous methylsulfonylmethane (MSM) solution (2.5, 5, 10, and 20%) and the corresponding MH scaffolds were referred to as MH2.5, MH5, MH10, and MH20, respectively. The results showed that all MH scaffolds resulted in burst release of MSM for up to 7 d. Cellular experiments were conducted using MC3T3-E1 preosteoblast cells, which showed no significant difference between the MH2.5 scaffold and the control with respect to the rate of cell proliferation (p > 0.05). There was no significant difference between each group at day 4 for alkaline phosphatase (ALP) activity, though the MH2.5 group showed higher level of activity than other groups at day 10. Calcium deposition, using alizarin red staining, showed that cell mineralization was significantly higher in the MH2.5 scaffold than that in the HAp scaffold (p < 0.0001). This study indicated that the MH2.5 scaffold has potential for both osteoinduction and osteoconduction in bone regeneration.

scholarly journals Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

2020 ◽  
Author(s):  
Isabel Pereira ◽  
José Eduardo Pereira ◽  
Luís Maltez ◽  
Alexandra Rodrigues ◽  
Catarina Rodrigues ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Substitute ◽  
Bone Substitutes ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Micro Computed Tomography ◽  
Synthetic Bone Substitute ◽  
In Situ Forming ◽  
Bioactive Agents ◽  
In Situ Forming Hydrogels

Abstract The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike®) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike® granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.

Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

2007 ◽  
Vol 30 (3) ◽  
pp. 405-410 ◽  
Author(s):  
Chang Seok Ki ◽  
Sook Young Park ◽  
Hyun Jeong Kim ◽  
Hong-Moon Jung ◽  
Kyung Mi Woo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
High Porosity
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