Chlorella sorokiniana: Effect of Nitrate Replete Concentration on Biomass Yield, Cell and Nitrate Concentration

2019 ◽  
Vol 797 ◽  
pp. 365-372
Author(s):  
Nor Azalina Zakariah ◽  
Norazah Abd Rahman ◽  
Noor Amelina Abdul Rahim
Keyword(s):  
Optical Density ◽  
Lipid Content ◽  
Biomass Yield ◽  
Cell Concentration ◽  
Nitrate Concentration ◽  
Basal Medium ◽  
Chlorella Sorokiniana ◽  
High Biomass ◽  
Nitrate Concentrations ◽  
High Biomass Yield

Effect of various nitrate replete concentrations have been investigated in terms of biomass yield, cell and nitrate concentrations. Media used in this study is Bold’s basal medium which contains nitrate that act as nutrient. Its concentrations have been varied for obtaining the high biomass yield. The nitrate concentrations used were 30 mg nitrate/L, 35 mg nitrate/L, 40 mg nitrate/L, 45 mg nitrate/L and 50 mg nitrate/L) with 30 mg nitrate/L as a control. Microalgae Chlorella sp. is cultured and aerated in a Schott bottle with presents of light. Results showed that the best concentration to obtain highest biomass yield was 45 mg nitrate/L. It also gave the highest optical density reading at day 9 with 2.100 ± 0.070 and showed the highest cell concentration with 703 ± 29 x 106 cells/mL. Dry algae produced by this concentration after the end of the cycle was 291 ± 9 mg which was the highest compared to other concentration. It is suggested that as the biomass yield is increased by using 45 mg nitrate/L concentration, other methods to increase lipid content can be paired with nitrate replete method and can be further studied in the future.

scholarly journals A High Biomass Yield and Whole Crop Silage Rice Cultivar ‘Mogyang’

2013 ◽  
Vol 45 (4) ◽  
pp. 405-409 ◽  
Author(s):  
Chang-Ihn Yang ◽  
Sang-Bok Lee ◽  
Yong-Jae Won ◽  
Eok-Keun Ahn ◽  
Myeong-Ki Kim ◽  
...  
Keyword(s):  
Biomass Yield ◽  
Rice Cultivar ◽  
High Biomass ◽  
High Biomass Yield

High biomass yield energy sorghum: developing a genetic model for C4 grass bioenergy crops

2012 ◽  
Vol 6 (6) ◽  
pp. 640-655 ◽  
Author(s):  
Sara N. Olson ◽  
Kimberley Ritter ◽  
William Rooney ◽  
Armen Kemanian ◽  
Bruce A. McCarl ◽  
...  
Keyword(s):  
Biomass Yield ◽  
Genetic Model ◽  
Bioenergy Crops ◽  
High Biomass ◽  
C4 Grass ◽  
Energy Sorghum ◽  
High Biomass Yield

Murphy Oat

2002 ◽  
Vol 82 (3) ◽  
pp. 555-557 ◽  
Author(s):  
Solomon Kibite, V. Baron ◽  
D. McCartney ◽  
N. Fairey ◽  
G. Clayton
Keyword(s):  
Key Words ◽  
Avena Sativa ◽  
Biomass Yield ◽  
Research Centre ◽  
High Biomass ◽  
Lodging Resistance ◽  
Cultivar Description ◽  
High Biomass Yield

Murphy is a high-yielding forage oat (Avena sativa L.) cultivar developed by Agriculture and Agri-Food Canada, Lacombe Research Centre, Lacombe, AB, and released in 2000. It is a late-maturing cultivar with high biomass yield and good lodging resistance. It was developed from a cross of Waldern/IH-1863-515. Murphy is well adapted to Alberta and the rust-free areas of Saskatchewan. Key words: Avena sativa, forage oat (spring), cultivar description

Breeding for High Biomass Yield in Spring Barley

Crop Science ◽  
1990 ◽  
Vol 30 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Lakhdar Boukerrou ◽  
Donald D. Rasmusson
Keyword(s):  
Biomass Yield ◽  
Spring Barley ◽  
High Biomass ◽  
High Biomass Yield

scholarly journals Variation in biomass related variables of reed canary grass

2003 ◽  
Vol 12 (3-4) ◽  
pp. 213-225 ◽  
Author(s):  
M. SAHRAMAA ◽  
H. IHAMÄKI ◽  
L. JAUHIAINEN
Keyword(s):  
Plant Height ◽  
Biomass Yield ◽  
Local Group ◽  
Yield Potential ◽  
Reed Canary Grass ◽  
High Biomass ◽  
Panicle Number ◽  
Shoot Number ◽  
Canary Grass ◽  
High Biomass Yield

Reed canary grass, Phalaris arundinacea L., is a relatively new biomass crop in northern Europe, which produces raw material for bioenergy and paper pulp. Breeding reed canary grass for industrial purposes is under way in the absence of domestic cultivars being available. Knowledge of the extent of variation in biomass related traits is a basic requirement of the breeding programme. The aim of this study was to describe variation in biomass related traits and evaluate the relationships among the variables. Field experiment was carried out between 1994 and 1998 in Finland. Research material included wild and elite populations, which were divided into ten groups according to their origin. Biomass yield, plant fractions, shoot number, node number, leaf area and overwintering ability were measured. Panicle number, plant height and seed ripening were included to the analyses of the relationships. Results indicated the high biomass yield potential of reed canary grass, reaching over 13 t DM ha-1 in the fourth year after establishment. Elite material and a local group from southern Finland had the highest biomass yield, whereas the northernmost local group had the lowest. Three factors established accounted for 45% of the variance and they were defined as “high biomass yield”, “leaf-shoot relationship” and “fast development”. The first factor indicated positive connections among biomass yield, panicle number, plant height, straw fraction and node fraction. This study indicated variation in agronomic traits of reed canary grass, which enables breeding of new cultivars with desired trait combinations.;

scholarly journals Effect of Different Cultivation Modes (Photoautotrophic, Mixotrophic, and Heterotrophic) on the Growth of Chlorella sp. and Biocompositions

2021 ◽  
Vol 9 ◽  
Author(s):  
Hyun-Sik Yun ◽  
Young-Saeng Kim ◽  
Ho-Sung Yoon
Keyword(s):  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Inorganic Carbon ◽  
Biomass Yield ◽  
Carbon Sources ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Raw Material ◽  
Heterotrophic Cultivation ◽  
Mixotrophic Conditions

In the past, biomass production using microalgae culture was dependent on inorganic carbon sources as microalgae are photosynthetic organisms. However, microalgae utilize both organic and inorganic carbon sources, such as glucose. Glucose is an excellent source of organic carbon that enhances biomass yield and the content of useful substances in microalgae. In this study, photoautotrophic, mixotrophic, and heterotrophic cultivation conditions were applied to three well-known strains of Chlorella (KNUA104, KNUA114, and KNUA122) to assess biomass productivity, and compositional changes (lipid, protein, and pigment) were evaluated in BG11 media under photoautotrophic, mixotrophic, and heterotrophic conditions utilizing different initial concentrations of glucose (5, 10, 15, 20, and 25 g L−1). Compared to the photoautotrophic condition (biomass yield: KNUA104, 0.35 ± 0.04 g/L/d; KNUA114, 0.40 ± 0.08 g/L/d; KNUA122, 0.38 ± 0.05 g/L/d) glucose was absent, and the biomass yield improved in the mixotrophic (glucose: 20 g L−1; biomass yield: KNUA104, 2.99 ± 0.10 g/L/d; KNUA114, 5.18 ± 0.81 g/L/d; KNUA122, 5.07 ± 0.22 g/L/d) and heterotrophic conditions (glucose: 20 g L−1; biomass yield: KNUA104, 1.72 ± 0.26 g/L/d; KNUA114, 4.26 ± 0.27 g/L/d; KNUA122, 4.32 ± 0.32 g/L/d). All strains under mixotrophic and heterotrophic conditions were optimally cultured when 15–20 g L−1 initial glucose was provided. Although bioresourse productivity improved under both mixotrophic and heterotrophic conditions where mixotrophic conditions were found to be optimal as the yields of lipid and pigment were also enhanced. Protein content was less affected by the presence of light or the concentration of glucose. Under mixotrophic conditions, the highest lipid content (glucose: 15 g L−1; lipid content: 68.80 ± 0.54%) was obtained with Chlorella vulgaris KNUA104, and enhanced pigment productivity of Chlorella sorokiniana KNUA114 and KNUA122 (additional pigment yield obtained with 15 g L−1 glucose: KNUA 114, 0.33 ± 0.01 g L−1; KNUA122, 0.21 ± 0.01 g L−1). Also, saturated fatty acid (SFA) content was enhanced in all strains (SFA: KNUA104, 29.76 ± 1.31%; KNUA114, 37.01 ± 0.98%; KNUA122, 33.37 ± 0.17%) under mixotrophic conditions. These results suggest that mixotrophic cultivation of Chlorella vulgaris and Chlorella sorokiniana could improve biomass yield and the raw material quality of biomass.

The Influence of Various pH Values on Monoraphidium sp. FXY-10 Growth and Lipid Parameters in Autotrophic and Heterotrophic Conditions

2013 ◽  
Vol 864-867 ◽  
pp. 60-66
Author(s):  
Yu Qiang Gao ◽  
Qiu Yan Zhang ◽  
Li Huang ◽  
Lin Wang ◽  
Xu Ya Yu
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Biomass Yield ◽  
Lipid Yield ◽  
Culture Time ◽  
Ph Values ◽  
Heterotrophic Condition ◽  
Influence Of Ph ◽  
Lipid Parameters ◽  
High Biomass Yield

The influence of pH on Monoraphidium sp. FXY-10 growth, lipid content, lipid yield, biomass yield, and fatty acid composition is studied in autotrophic and heterotrophic conditions. The results reveal that Monoraphidium sp. FXY-10 can grow better in an acidic environment. Under autotrophic and heterotrophic conditions, the culture time is 37 and 9 day, respectively. And the maximum biomass of algal cells is 32 and 367mg/l/d with the lipid content in autotrophic and heterotrophic conditions reached to 49% and 39%, respectively, with high biomass yield, lipid yield, the saturated fatty acid and monounsaturated fatty acid under heterotrophic condition, proving that the algal cells are a viable material for the production of biodiesel.

scholarly journals The Effect of Steam Explosion Treatment on Technical Hemp Fibres

2015 ◽  
Vol 1 ◽  
pp. 230 ◽  
Author(s):  
Silvija Kukle ◽  
Jānis Grāvītis ◽  
Anna Putniņa ◽  
Anete Stikute
Keyword(s):  
Steam Explosion ◽  
Biomass Yield ◽  
High Strength ◽  
Renewable Resource ◽  
High Biomass ◽  
Environment Friendly ◽  
Experimental Part ◽  
Wide Range ◽  
Hemp Fibres ◽  
High Biomass Yield

As hemp is a renewable resource with the high biomass yield it could be considered as potential abundant local biomass material for a wide range of applications. In this article hemp fibres architecture as a source of high strength cellulose are analysed. In experimental part steam explosion technology is applied to disintegrate technical hemp fibres to elementary fibres with the aim to find out the best way of procedure without usage to environment harmful chemical pre-treatments and looking forward to solve problems on further nano-level environment friendly hemp cellulose disintegration.

Sign in / Sign up

Export Citation Format

Share Document