The Effect of theophylline on eotaxin mRNA expression in pulmonary epithelial cell line A549

2000 ◽  
Vol 48 (6) ◽  
pp. 898
Author(s):  
Min Soo Han ◽  
Jee Hong Yoo ◽  
Hong Mo Kang
Keyword(s):  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Pulmonary Epithelial Cell

The lack of attachment of transformed embryonic lung epithelial cells to collagen I is corrected by fibronectin and FXIII

1986 ◽  
Vol 86 (1) ◽  
pp. 95-107
Author(s):  
M. Paye ◽  
C.M. Lapiere
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Lung Epithelial Cells ◽  
Collagen I ◽  
Epithelial Cell Line ◽  
Embryonic Lung ◽  
Lung Epithelial ◽  
Minimal Concentration ◽  
Pulmonary Epithelial Cell

PER cells, a transformed pulmonary epithelial cell line that adhered to a large extent to a fibronectin substratum, were found to be attachment-deficient to collagen I. Although fibronectin can bind to collagen I monomers and polymers, the addition of exogenous fibronectin in the attachment medium induced the adhesion of these cells to collagen I polymers but not to monomers. By adding the transglutaminase of blood coagulation, FXIII, in the presence of fibronectin, the attachment of PER cells to collagen I monomers could be recovered while the minimal concentration of fibronectin needed to promote their adhesion to polymers was lowered. These studies indicate that FXIII enhances the fibronectin-mediated attachment of PER cells to collagen I.

Mechanical strain-induced c-fos expression in pulmonary epithelial cell line A549

2006 ◽  
Vol 347 (1) ◽  
pp. 369-372 ◽  
Author(s):  
Binwu Ying ◽  
Hong Fan ◽  
Fuqiang Wen ◽  
Dan Xu ◽  
Daishun Liu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mechanical Strain ◽  
Epithelial Cell Line ◽  
Pulmonary Epithelial Cell ◽  
Fos Expression

Modulation of endothelin-1 production by a pulmonary epithelial cell line

1994 ◽  
Vol 48 (11) ◽  
pp. 2065-2071 ◽  
Author(s):  
Eduardo Calderón ◽  
Celso E. Gómez-Sánchez ◽  
Eduardo N. Cozza ◽  
Mingyi Zhou ◽  
Ronald G. Coffey ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Endothelin 1 ◽  
Pulmonary Epithelial Cell

Transporter mRNA Expression in a Conditionally Immortalized Rat Small Intestine Epithelial Cell Line (TR-SIE)

2004 ◽  
Vol 19 (4) ◽  
pp. 264-269 ◽  
Author(s):  
Ken-ichi Hosoya ◽  
Masatoshi Tomi ◽  
Megumi Takayama ◽  
Yuko Komokata ◽  
Daisuke Nakai ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Rat Small Intestine

scholarly journals Clarithromycin Suppresses Human Respiratory Syncytial Virus Infection-InducedStreptococcus pneumoniaeAdhesion and Cytokine Production in a Pulmonary Epithelial Cell Line

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Shin-ichi Yokota ◽  
Tamaki Okabayashi ◽  
Satoshi Hirakawa ◽  
Hiroyuki Tsutsumi ◽  
Tetsuo Himi ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Human Respiratory Syncytial Virus ◽  
Receptor Expression ◽  
Host Cells ◽  
Epithelial Cell Line ◽  
Paf Receptor ◽  
Pulmonary Epithelial Cell ◽  
Syncytial Virus

Human respiratory syncytial virus (RSV) sometimes causes acute and severe lower respiratory tract illness in infants and young children. RSV strongly upregulates proinflammatory cytokines and the platelet-activating factor (PAF) receptor, which is a receptor forStreptococcus pneumoniae, in the pulmonary epithelial cell line A549. Clarithromycin (CAM), which is an antimicrobial agent and is also known as an immunomodulator, significantly suppressed RSV-induced production of interleukin-6, interleukin-8, and regulated on activation, normal T-cell expressed and secreted (RANTES). CAM also suppressed RSV-induced PAF receptor expression and adhesion of fluorescein-labeledS. pneumoniaecells to A549 cells. The RSV-inducedS. pneumoniaeadhesion was thought to be mediated by the host cell’s PAF receptor. CAM, which exhibits antimicrobial and immunomodulatory activities, was found in this study to suppress the RSV-induced adhesion of respiratory disease-causing bacteria,S. pneumoniae, to host cells. Thus, CAM might suppress immunological disorders and prevent secondary bacterial infections during RSV infection.

Fas-induced death of a murine pulmonary epithelial cell line: modulation by inflammatory cytokines

1999 ◽  
Vol 13 (6) ◽  
pp. 656-661 ◽  
Author(s):  
Alexandre Trifilieff ◽  
Yasushi Futjitani ◽  
Anthony J. Coyle ◽  
Claude Bertrand
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Inflammatory Cytokines ◽  
Epithelial Cell Line ◽  
Pulmonary Epithelial Cell

Global transcriptional characterization of a mouse pulmonary epithelial cell line for use in genetic toxicology

2009 ◽  
Vol 23 (5) ◽  
pp. 816-833 ◽  
Author(s):  
M. Lynn Berndt-Weis ◽  
Lisa M. Kauri ◽  
Andrew Williams ◽  
Paul White ◽  
George Douglas ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Genetic Toxicology ◽  
Pulmonary Epithelial Cell

scholarly journals Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line

2013 ◽  
Vol 79 (7) ◽  
pp. 2225-2232 ◽  
Author(s):  
Murphy Lam-Yim Wan ◽  
Chit-Shing Jackson Woo ◽  
Kevin J. Allen ◽  
Paul C. Turner ◽  
Hani El-Nezami
Keyword(s):  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Defense Mechanisms ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epithelial Cell Line ◽  
Content Type ◽  
Antimicrobial Effects ◽  
Food And Feed

ABSTRACTDefensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four commonFusariumtoxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA), and fumonisin B1 (FB1), on the mRNA expression, protein secretion, and corresponding antimicrobial effects of porcine β-defensins 1 and 2 (pBD-1 and pBD-2) using a porcine jejunal epithelial cell line, IPEC-J2. In general, upregulation of pBD-1 and pBD-2 mRNA expression occurred following exposure toFusariumtoxins, individually and in mixtures (P< 0.05). However, no significant increase in secreted pBD-1 and pBD-2 protein levels was observed, as measured by enzyme-linked immunosorbent assay (ELISA). Supernatants from IPEC-J2 cells exposed to toxins, singly or in combination, however, possessed significantly less antimicrobial activity againstEscherichia colithan untreated supernatants. When single toxins and two-toxin combinations were assessed, toxicity effects were shown to be nonadditive (including synergism, potentiation, and antagonism), suggesting interactive toxin effects when cells are exposed to mycotoxin combinations. The results show thatFusariumtoxins, individually and in mixtures, activate distinct antimicrobial defense mechanisms possessing the potential to alter the intestinal microbiota through diminished antimicrobial effects. Moreover, by evaluating toxin mixtures, this improved understanding of toxin effects will enable more effective risk assessments for common mycotoxin combinations observed in contaminated food and feed.

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