scholarly journals Shiga Toxin Produced by EnterohemorrhagicEscherichia coliInhibits PI3K/NF-κB Signaling Pathway in Globotriaosylceramide-3-Negative Human Intestinal Epithelial Cells

2007 ◽  
Vol 178 (12) ◽  
pp. 8168-8174 ◽  
Author(s):  
Alain P. Gobert ◽  
Marjolaine Vareille ◽  
Anne-Lise Glasser ◽  
Thomas Hindré ◽  
Thibaut de Sablet ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathway ◽  
Shiga Toxin ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

M1614 Expression of Delta Ligands Is Regulated By Notch-Hes1 Signaling Pathway in Human Intestinal Epithelial Cells

2009 ◽  
Vol 136 (5) ◽  
pp. A-395
Author(s):  
Junko Akiyama ◽  
Ryuichi Okamoto ◽  
Kiichiro Tsuchiya ◽  
Tetsuya Nakamura ◽  
Mamoru Watanabe
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathway ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Cell invasion and survival of Shiga toxin-producing Escherichia coli within cultured human intestinal epithelial cells

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_8) ◽  
pp. 1683-1694 ◽  
Author(s):  
Fabiana Cordeiro ◽  
Rita Ifuoe K. da Silva ◽  
Thaís L. Z. Vargas-Stampe ◽  
Aloysio M. F. Cerqueira ◽  
João R. C. Andrade
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Cell Invasion ◽  
Shiga Toxin ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

scholarly journals Macropinocytosis in Shiga toxin 1 uptake by human intestinal epithelial cells and transcellular transcytosis

2009 ◽  
Vol 296 (1) ◽  
pp. G78-G92 ◽  
Author(s):  
Irina Malyukova ◽  
Karen F. Murray ◽  
Chengru Zhu ◽  
Edgar Boedeker ◽  
Anne Kane ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Shiga Toxin ◽  
Intestinal Epithelial Cells ◽  
Enterohemorrhagic Escherichia Coli ◽  
Apical Surface ◽  
Intestinal Epithelial ◽  
Shiga Toxins ◽  
E Coli ◽  
Human Intestinal Epithelial Cells ◽  
Toxin Uptake

Shiga toxin 1 and 2 production is a cardinal virulence trait of enterohemorrhagic Escherichia coli infection that causes a spectrum of intestinal and systemic pathology. However, intestinal sites of enterohemorrhagic E. coli colonization during the human infection and how the Shiga toxins are taken up and cross the globotriaosylceramide (Gb3) receptor-negative intestinal epithelial cells remain largely uncharacterized. We used samples of human intestinal tissue from patients with E. coli O157:H7 infection to detect the intestinal sites of bacterial colonization and characterize the distribution of Shiga toxins. We further used a model of largely Gb3-negative T84 intestinal epithelial monolayers treated with B-subunit of Shiga toxin 1 to determine the mechanisms of non-receptor-mediated toxin uptake. We now report that E. coli O157:H7 were found at the apical surface of epithelial cells only in the ileocecal valve area and that both toxins were present in large amounts inside surface and crypt epithelial cells in all tested intestinal samples. Our in vitro data suggest that macropinocytosis mediated through Src activation significantly increases toxin endocytosis by intestinal epithelial cells and also stimulates toxin transcellular transcytosis. We conclude that Shiga toxin is taken up by human intestinal epithelial cells during E. coli O157:H7 infection regardless of the presence of bacterial colonies. Macropinocytosis might be responsible for toxin uptake by Gb3-free intestinal epithelial cells and transcytosis. These observations provide new insights into the understanding of Shiga toxin contribution to enterohemorrhagic E. coli-related intestinal and systemic diseases.

scholarly journals Spirulina Crude Protein Promotes the Migration and Proliferation in IEC-6 Cells by Activating EGFR/MAPK Signaling Pathway

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 205
Author(s):  
Su-Jin Jeong ◽  
Jeong-Wook Choi ◽  
Min-Kyeong Lee ◽  
Youn-Hee Choi ◽  
Taek-Jeong Nam
Keyword(s):  
Cell Cycle ◽  
Epithelial Cells ◽  
Signaling Pathway ◽  
Crude Protein ◽  
Cell Cycle Progression ◽  
Intestinal Epithelial Cells ◽  
Mapk Signaling ◽  
S Phase ◽  
Intestinal Epithelial ◽  
Cycle Progression

Spirulina is a type of filamentous blue-green microalgae known to be rich in nutrients and to have pharmacological effects, but the effect of spirulina on the small intestine epithelium is not well understood. Therefore, this study aims to investigate the proliferative effects of spirulina crude protein (SPCP) on a rat intestinal epithelial cells IEC-6 to elucidate the mechanisms underlying its effect. First, the results of wound-healing and cell viability assays demonstrated that SPCP promoted migration and proliferation in a dose-dependent manner. Subsequently, when the mechanisms of migration and proliferation promotion by SPCP were confirmed, we found that the epidermal growth factor receptor (EGFR) and mitogen-activated protein (MAPK) signaling pathways were activated by phosphorylation. Cell cycle progression from G0/G1 to S phase was also promoted by SPCP through upregulation of the expression levels of cyclins and cyclin-dependent kinases (Cdks), which regulate cell cycle progression to the S phase. Meanwhile, the expression of cyclin-dependent kinase inhibitors (CKIs), such as p21 and p27, decreased with SPCP. In conclusion, our results indicate that activation of EGFR and its downstream signaling pathway by SPCP treatment regulates cell cycle progression. Therefore, these results contribute to the research on the molecular mechanism for SPCP promoting the migration and proliferation of rat intestinal epithelial cells.

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