Efectos del ácido giberélico, N6-bencilaminopurina y fluridona sobre la germinación in vitro de Aristotelia chilensis

This work aimed to identify the bioactive compounds present in adult maqui (Aristotelia chilensis) leaves from different stages of development and seasons of the year and compare them with leaves obtained from maqui plants grown in vitro. The qualitative and quantitative analysis of maqui leaf extracts by HPLC-DAD-ESI-MSn showed the presence of different polyphenolic compounds classified into galloyl and caffeoyl quinic acids, ellagitannins and ellagic acid- and flavonoid-derivatives. In general, the total phenolic content of the in vitro samples was higher than that of ex vitro samples, whereas the total flavonoid content was higher in winter basal leaves. Additionally, the analysis by HPLC-MS showed that the extract from spring basal leaves was enriched in quercetin, catechin, kaempferol and 3-caffeoyl quinic acids, while in the in vitro leaves extract, quercetin was not present. As regards lipophilic compounds identified by GC/MS, the samples of in vitro leaves showed a high presence of α-tocopherol and β-sitosterol. In contrast, the samples of adult leaves presented a hight level of linolenic and linoleic acids. These results suggest that maqui leaves could be an excellent source of antioxidants and lipophilic compounds for many industries, such as the nutraceutical and pharmaceutical industries.

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Elicitation of Phenylpropanoids in Maqui (Aristotelia chilensis [Mol.] Stuntz) Plants Micropropagated in Photomixotrophic Temporary Immersion Bioreactors (TIBs).

10.21203/rs.3.rs-255813/v1 ◽

2021 ◽

Author(s):

Giulia E Trentini ◽

Makarena Rojas ◽

Daniela Gajardo ◽

Débora Alburquenque ◽

Evelyn Villagra ◽

...

Keyword(s):

Culture Medium ◽

Wild Plants ◽

Control Treatment ◽

Multiplication Rate ◽

Fresh Weight ◽

Temporary Immersion ◽

Air Treatment ◽

The Third ◽

Aristotelia Chilensis

Abstract A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m− 2s− 1. The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO2 do not differ significantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air. Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not significant differences in polyphenols and anthocyanin contents were verified between the treatments of maqui in TIBs + ABA and controls. The non-significant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no significant differences by the ORAC test from day 5 to day 15 of the study.

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In vitro and in vivo anti-diabetic effects of anthocyanins from Maqui Berry (Aristotelia chilensis)

Food Chemistry ◽

10.1016/j.foodchem.2011.08.066 ◽

2012 ◽

Vol 131 (2) ◽

pp. 387-396 ◽

Cited By ~ 122

Author(s):

Leonel E. Rojo ◽

David Ribnicky ◽

Sithes Logendra ◽

Alex Poulev ◽

Patricio Rojas-Silva ◽

...

Keyword(s):

Aristotelia Chilensis ◽

Maqui Berry

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Assessment of polyphenolic profile stability and changes in the antioxidant potential of maqui berry (Aristotelia chilensis (Molina) Stuntz) during in vitro gastrointestinal digestion

Industrial Crops and Products ◽

10.1016/j.indcrop.2016.09.057 ◽

2016 ◽

Vol 94 ◽

pp. 774-782 ◽

Cited By ~ 50

Author(s):

Raquel Lucas-Gonzalez ◽

Sergio Navarro-Coves ◽

José A. Pérez-Álvarez ◽

Juana Fernández-López ◽

Loreto A. Muñoz ◽

...

Keyword(s):

Antioxidant Potential ◽

Gastrointestinal Digestion ◽

In Vitro Gastrointestinal Digestion ◽

Aristotelia Chilensis ◽

Maqui Berry ◽

Polyphenolic Profile

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Human erythrocytes are affected in vitro by flavonoids of Aristotelia chilensis (Maqui) leaves

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2008.07.005 ◽

2008 ◽

Vol 363 (1-2) ◽

pp. 85-90 ◽

Cited By ~ 45

Author(s):

Mario Suwalsky ◽

Pedro Vargas ◽

Marcia Avello ◽

Fernando Villena ◽

Carlos P. Sotomayor

Keyword(s):

Human Erythrocytes ◽

Aristotelia Chilensis

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Evaluation of protective effect of different dietary fibers on polyphenolic profile stability of maqui berry (Aristotelia chilensis(Molina) Stuntz) duringin vitrogastrointestinal digestion

Food & Function ◽

10.1039/c7fo01671a ◽

2018 ◽

Vol 9 (1) ◽

pp. 573-584 ◽

Cited By ~ 14

Author(s):

Manuel Viuda-Martos ◽

Raquel Lucas-Gonzalez ◽

Carmen Ballester-Costa ◽

José A. Pérez-Álvarez ◽

Loreto A. Muñoz ◽

...

Keyword(s):

Protective Effect ◽

Polyphenolic Compounds ◽

Dietary Fibers ◽

Gastrointestinal Digestion ◽

Aristotelia Chilensis ◽

Maqui Berry ◽

The Stability ◽

Polyphenolic Profile

We investigate the protective effect of dietary fibers on the recovery and bioaccessibility indexes, and the stability of polyphenolic compounds of maqui berry powder subjected toin vitrogastrointestinal digestion.

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Ultrastructural Investigations of the Contractile Filaments of Amoebae

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050950 ◽

1974 ◽

Vol 32 ◽

pp. 188-189

Author(s):

P.L. Moore

Keyword(s):

Cytoplasmic Streaming ◽

Three Dimensional ◽

Freeze Fracture ◽

Amoeboid Movement ◽

Different Types ◽

Chemical Conditions ◽

Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

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In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050676 ◽

1974 ◽

Vol 32 ◽

pp. 132-133

Author(s):

John J. Wolosewick ◽

John H. D. Bryan

Keyword(s):

Endoplasmic Reticulum ◽

Smooth Endoplasmic Reticulum ◽

Nuclear Ring ◽

Rough Er ◽

Distal Direction ◽

In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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