scholarly journals Esterase profiling and molecular identification of yeasts isolated from different environmental samples from Morocco

2019 ◽  
Vol 92 (2) ◽  
Author(s):  
Lamya El Aamri ◽  
Fabio Scordino ◽  
Cinzia Barresi ◽  
Orazio Romeo ◽  
Giuseppe Criseo ◽  
...  
Keyword(s):  
Olive Oil ◽  
Environmental Samples ◽  
Qualitative Method ◽  
Agar Plate ◽  
Its2 Region ◽  
Rhodotorula Mucilaginosa ◽  
Wickerhamomyces Anomalus ◽  
Black Olive ◽  
Esterase Production

One hundred and six fungal strains were isolated from different environmental samples (fresh olive oil cake, exhausted olive oil cake, black olive, rancid butter samples, rotten bread and Roquefort) collected from the region of Meknes, Morocco (coordinates: 33°53′42″N 5°33′17″W). Yeast isolates were tested for their esterase production ability using a qualitative method based on Tween agar plate assay. Enzymatic activity was also confirmed by a quantitative method relying on esterase production in liquid medium (6 days at 28°C with shaking). Molecular characterization of the selected esterase-producing yeasts was performed by sequencing the internal transcribed spacer 1 (ITS1), 5.8S and ITS2 region of the rDNA. A total of five different species were identified in this study: Candida aaseri (LE.26, LE.27 and LE.31 strains), Wickerhamomyces anomalus (LE.106, LE.112 and LE.115 strains), Metschnikowia rancensis (LE.153 strain), Pichia sp., (LE.102) and Rhodotorula mucilaginosa (LE.171 strain). Esterase production in C. aaseri and W. anomalus was found to be straindependent, while for M. rancensis this represents the first study reporting this species as an esterase producer.

scholarly journals Characterization of antioxidant olive oil biophenols by spectroscopic methods

2010 ◽  
Vol 91 (2) ◽  
pp. 309-314 ◽  
Author(s):  
Fátima Paiva-Martins ◽  
Vera Rodrigues ◽  
Rita Calheiros ◽  
Maria PM Marques
Keyword(s):  
Olive Oil ◽  
Spectroscopic Methods

scholarly journals Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus

2014 ◽  
Vol 57 (4) ◽  
pp. 587-594 ◽  
Author(s):  
María Alicia Martos ◽  
Ana Paula Butiuk ◽  
Natalia Lorena Rojas ◽  
Roque Alberto Hours
Keyword(s):  
Purification And Characterization ◽  
Wickerhamomyces Anomalus

scholarly journals Isolation and characterization of arsenic resistant soil bacteria and their effects on germination of rice under arsenic contamination

2015 ◽  
Vol 2 (2) ◽  
pp. 229-237
Author(s):  
Istiaq Ahmed ◽  
Md Tofazzal Islam ◽  
Md Akhter Hossain Chowdhury ◽  
Md Kamruzzaman
Keyword(s):  
Contaminated Soils ◽  
Sodium Arsenite ◽  
Agar Plate ◽  
Rice Seedling ◽  
Resistant Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Resistant Strains

This study was carried out to isolate, screen and characterize arsenic (As) resistant bacteria from As contaminated soils of Dumrakandi and Matlab under Faridpur and Chandpur districts and to evaluate their efficiency in reducing As toxicity against rice seedlings during germination. Thirteen strains were isolated from the soils which showed resistance to different levels of sodium arsenite (viz. 5, 10, 20 and 40 mM) in both agar plate and broth assay using BSMY I media. Among the isolates, BTL0011, BTL0012, BTL0015 and BTL0022 showed highest resistance to 40 mM sodium arsenite. Gram staining and KOH solubility test revealed that five strains were gram positive and rest eight was gram negative. They grew well in the liquid media at pH 5.5 to 8.5. In-vitro rice seedling bioassay with two superior isolates (BTL0011 and BTL0022) revealed that As resistant strains significantly enhanced seed germination of BRRI dhan29 and BRRI dhan47 at 60 ppm As. This study was laid out in CRD with three replications. The performance of BTL 0022 was superior to BTL0011. The overall results suggest that BTL0011 and BTL0022 can be used for bioremediation of As contaminated soils and to increase the germination and seedling growth of rice in As contaminated soils.Res. Agric., Livest. Fish.2(2): 229-237, August 2015

scholarly journals Physico-chemical and quality characterization of pulp oil from two varieties of pili (Canarium ovatum Engl.) from Bicol, Philippines

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1937-1946
Author(s):  
M.M.D.R. Tugay ◽  
L.E. Mopera ◽  
E.B. Esguerra ◽  
K.A.T. Castillo-Israel
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Coconut Oil ◽  
Acid Value ◽  
Extra Virgin Olive Oil ◽  
Quality Characteristics ◽  
Physico Chemical ◽  
Dark Color

This study aimed to characterize and compare pili (Canarium ovatum Engl.) pulp oil from two different varieties of pili fruits in Bicol, Philippines namely M. Orolfo and Orbase varieties for possible utilization into oil-based products. The effects of varietal differences in pili fruits on physical, chemical and quality characteristics of its pulp oil were determined. These two oils were also compared with control oils, commercially available coconut oil and extra virgin olive oil. Pili pulp oil from M. Orolfo had dark color while Orbase had color close to extra virgin olive oil. The two varieties did not significantly differ from each other in terms of refractive index, moisture and volatile matters, acid value, iodine value, saponification number and percent unsaponifiable matter but significantly differed from coconut oil and extra virgin olive oil. On the other hand, the two varieties significantly differed from each other in terms of peroxide value, Vitamin A and α-tocopherol contents. In terms of fatty acid profile, high amounts of palmitic acid were determined in both pili varieties (19-25%) compared with coconut oil (6.34%). Oleic acid in Orbase was 71.5% while M. Orolfo had 58.1%, which are comparable with extra virgin olive oil (77.9%). Pili pulp oils from M. Orolfo and Orbase can be utilized into oilbased products because its chemical and quality characteristics are within the standard. Both can be stored for a longer period of time and healthier in terms of fatty acid composition and natural antioxidant content.

scholarly journals Microbial diversity characterization of seawater in a pilot study using Oxford Nanopore Technologies long-read sequencing

2020 ◽  
Author(s):  
Michael Liem ◽  
Tonny Regensburg-Tuïnk ◽  
Christiaan Henkel ◽  
Hans Jansen ◽  
Herman Spaink
Keyword(s):  
Microbial Diversity ◽  
Environmental Samples ◽  
Sea Water ◽  
Flow Cells ◽  
Oxford Nanopore ◽  
Challenging Tasks ◽  
Long Read ◽  
Close Relatives ◽  
Oxford Nanopore Technologies

Abstract Objective: Currently the majority of non-culturable microbes in sea water are yet to be discovered, Nanopore offers a solution to overcome the challenging tasks to identify the genomes and complex composition of oceanic microbiomes. In this study we evaluate the utility of Oxford Nanopore Technologies (ONT) sequencing to characterize microbial diversity in seawater from multiple locations. We compared the microbial species diversity of retrieved environmental samples from two different locations and time points.Results: With only three ONT flow cells we were able to identify thousands of organisms, including bacteriophages, from which a large part at species level. It was possible to assemble genomes from environmental samples with Flye. In several cases this resulted in >1 Mbp contigs and in the particular case of a Thioglobus singularis species it even produced a near complete genome. k-mer analysis reveals that a large part of the data represents species of which close relatives have not yet been deposited to the database. These results show that our approach is suitable for scalable genomic investigations such as monitoring oceanic biodiversity and provides a new platform for education in biodiversity.

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