scholarly journals BIOINGEGNERIA DEL NERVO ARTIFICIALE

2012 ◽  
Author(s):  
Giorgio Terenghi
Keyword(s):  
Stem Cells ◽  
Schwann Cells ◽  
Adult Stem Cells ◽  
Surgical Techniques ◽  
Optimal Recovery ◽  
Nerve Reconstruction ◽  
Nano Technology ◽  
Nerve Conduits ◽  
New Strategy ◽  
Promising Development

At present, peripheral nerve injury represents a complex challenge for the surgeons, and the repair of these injuries often results in sub-optimal recovery and functional deficit. The neurobiology of nerve regeneration cannot be adequately addressed by the existing surgical techniques, and it has become apparent that tissue engineering and the creation of nerve conduits have significant potential to improve the results of nerve reconstruction. The use of novel biomaterials and more refined fabrication methods, by using micro- and nano-technology, are a promising development for these experimental constructs. Also, the use of cells transplanted into the conduit to create a bioartificial nerve graft represents a new development. Adult stem cells constitute the ideal alternative to Schwann cells, and among the various sources of stem cells with potential application for regenerative medicine the adipose tissue has been proven to be the most promising. Adult adipose-derived stem cells are easily obtained, they expand rapidly and can be differentiated to Schwann cells, hence they represent a new strategy for clinical application in order to improve the results of nerve re generation.

scholarly journals Co-Culturing Human Adipose Derived Stem Cells and Schwann Cells on Spider Silk—A New Approach as Prerequisite for Enhanced Nerve Regeneration

2018 ◽  
Vol 20 (1) ◽  
pp. 71 ◽  
Author(s):  
Annika Resch ◽  
Sonja Wolf ◽  
Anda Mann ◽  
Tamara Weiss ◽  
Alexandra-Larissa Stetco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Spider Silk ◽  
Cell Layer ◽  
Donor Site ◽  
Autologous Transplantation ◽  
Adipose Derived Stem Cells ◽  
Nerve Reconstruction ◽  
New Approach

Fast recovery is crucial for a successful nerve repair and an optimal functional outcome after peripheral nerve injury. Regarding donor site morbidity, autologous transplantation shows great limitations, which urge the need for alternative options in nerve reconstruction. Spider silk was reported as an advantageous material for cell adhesion, migration and proliferation, and its use in conduits is of great interest, especially in combination with cells to improve nerve regeneration. We here described the behavior of a co-culture of human Schwann cells and human adipose-derived stem cells (ADSCs) on spider silk as a new approach. After characterized by immunostaining ADSCs and Schwann cells were seeded in the co-culture on a spider silk scaffold and observed for 21 days. Results showed that cells were attached to the silk and aligned along the silk fibers. With further culture time, cells migrated along the silk and increased in number and formed an almost confluent cell layer. In immunostaining, results suggest that the cell layer was equally composed of ADSCs and Schwann cells. In conclusion, we showed that by providing a guiding structure for directed growth and cells to support nerve regeneration and remyelination, a valid alternative to autologous nerve grafts could have been found.

Sciatic Nerve Regeneration by Cocultured Schwann Cells and Stem Cells on Microporous Nerve Conduits

2013 ◽  
Vol 22 (11) ◽  
pp. 2029-2039 ◽  
Author(s):  
Lien-Guo Dai ◽  
Guo-Shiang Huang ◽  
Shan-Hui Hsu
Keyword(s):  
Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Nerve Conduits ◽  
Sciatic Nerve Regeneration

scholarly journals Emerging Perspectives in Scaffold for Tissue Engineering in Oral Surgery

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Gabriele Ceccarelli ◽  
Rossella Presta ◽  
Laura Benedetti ◽  
Maria Gabriella Cusella De Angelis ◽  
Saturnino Marco Lupi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Regeneration ◽  
Cell Biology ◽  
Adult Stem Cells ◽  
Materials Science ◽  
Oral Surgery ◽  
Surgical Techniques ◽  
Socket Preservation

Bone regeneration is currently one of the most important and challenging tissue engineering approaches in regenerative medicine. Bone regeneration is a promising approach in dentistry and is considered an ideal clinical strategy in treating diseases, injuries, and defects of the maxillofacial region. Advances in tissue engineering have resulted in the development of innovative scaffold designs, complemented by the progress made in cell-based therapies. In vitro bone regeneration can be achieved by the combination of stem cells, scaffolds, and bioactive factors. The biomimetic approach to create an ideal bone substitute provides strategies for developing combined scaffolds composed of adult stem cells with mesenchymal phenotype and different organic biomaterials (such as collagen and hyaluronic acid derivatives) or inorganic biomaterials such as manufactured polymers (polyglycolic acid (PGA), polylactic acid (PLA), and polycaprolactone). This review focuses on different biomaterials currently used in dentistry as scaffolds for bone regeneration in treating bone defects or in surgical techniques, such as sinus lift, horizontal and vertical bone grafts, or socket preservation. Our review would be of particular interest to medical and surgical researchers at the interface of cell biology, materials science, and tissue engineering, as well as industry-related manufacturers and researchers in healthcare, prosthetics, and 3D printing, too.

scholarly journals Past, Present, and Future of Nerve Conduits in the Treatment of Peripheral Nerve Injury

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Aikeremujiang Muheremu ◽  
Qiang Ao
Keyword(s):  
Stem Cells ◽  
Clinical Practice ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Neurotrophic Factors ◽  
Peripheral Nerve Injury ◽  
Functional Outcomes ◽  
Nerve Conduits ◽  
New Construction

With significant advances in the research and application of nerve conduits, they have been used to repair peripheral nerve injury for several decades. Nerve conduits range from biological tubes to synthetic tubes, and from nondegradable tubes to biodegradable tubes. Researchers have explored hollow tubes, tubes filled with scaffolds containing neurotrophic factors, and those seeded with Schwann cells or stem cells. The therapeutic effect of nerve conduits is improving with increasing choice of conduit material, new construction of conduits, and the inclusion of neurotrophic factors and support cells in the conduits. Improvements in functional outcomes are expected when these are optimized for use in clinical practice.

Characterization of Nerve Conduits Seeded with Neurons and Schwann Cells Derived from Hair Follicle Neural Crest Stem Cells

2011 ◽  
Vol 17 (13-14) ◽  
pp. 1691-1698 ◽  
Author(s):  
Haiyan Lin ◽  
Fang Liu ◽  
Chuansen Zhang ◽  
Zhiying Zhang ◽  
Zhengdong Kong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Hair Follicle ◽  
Schwann Cells ◽  
Neural Crest Stem Cells ◽  
Nerve Conduits

Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells

2012 ◽  
pp. 47-57 ◽  
Author(s):  
Cristina Mantovani ◽  
Giorgio Terenghi ◽  
Susan G. Shawcross
Keyword(s):  
Stem Cells ◽  
Schwann Cells ◽  
Adult Stem Cells

Cellular cardiomyoplasty using embryonic cardiomyocytes and adult stem cells

2005 ◽  
Vol 53 (S 3) ◽  
Author(s):  
W Röll ◽  
T Hashemi ◽  
M Breitbach ◽  
O Dewald ◽  
A Welz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Cellular Cardiomyoplasty ◽  
Embryonic Cardiomyocytes
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