scholarly journals Feline panleukopenia virus revisited : molecular characteristics and pathological lesions associated with three recent isolates

2000 ◽  
Vol 71 (3) ◽  
Author(s):  
M. Van Vuuren ◽  
A. Steinel ◽  
T. Goosen ◽  
E. Lane ◽  
J. Van der Lugt ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Domestic Cat ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Feline Panleukopenia Virus ◽  
Pathological Lesions ◽  
Polymerase Chain ◽  
Low Incidence ◽  
Canine Parvovirus Type 2

The low incidence of clinical signs or pathological lesions compatible with feline panleukopenia in cats has created the perception among practitioners that the disease has disappeared since the emergence of canine parvovirus type 2 in the late 1970s.Three parvoviruses that were recently isolated from a domestic cat and 2 cheetahs in cell culture or detected by means of the polymerase chain reaction were shown to be typical feline parvoviruses. Phylogenetic comparison with other FPV isolates did not reveal a particular African cluster.

Molecular characterisation of ovine herpesvirus type 2 (OvHV-2) in Turkey

2012 ◽  
Vol 60 (4) ◽  
pp. 521-527 ◽  
Author(s):  
Yakup Yildirim ◽  
Seval Bilge Dağalp ◽  
Volkan Yilmaz ◽  
Ali Faraji Majarashin
Keyword(s):  
Clinical Signs ◽  
Peripheral Blood Leukocyte ◽  
Malignant Catarrhal Fever ◽  
Molecular Characterisation ◽  
Laboratory Examination ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Polymerase Chain ◽  
Blood Leukocyte

In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.

scholarly journals Clinical and Virological Findings in Pups Naturally Infected by Canine Parvovirus Type 2 Glu-426 Mutant

2005 ◽  
Vol 17 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Nicola Decaro ◽  
Costantina Desario ◽  
Marco Campolo ◽  
Gabriella Elia ◽  
Vito Martella ◽  
...  
Keyword(s):  
Real Time ◽  
Virus Isolation ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Viral Shedding ◽  
Polymerase Chain ◽  
Loss Of Appetite ◽  
Hematological Changes ◽  
Canine Parvovirus Type 2

An outbreak of canine parvovirus type 2 infection caused by the Glu-426 mutant in 2 litters of pups is reported. The infected pups ( n = 6) were monitored daily for evidence of clinical signs and hematological changes and for the evaluation of viral shedding in the feces. The disease induced by the Glu-426 mutant was mild in all the infected pups. Vomiting and hemorrhagic diarrhea were not observed; however, the pups developed mucoid diarrhea (3.5 median days), depression (1.5 median days), and relative leukopenia and lymphopenia (2.5 median days). Fever and loss of appetite were observed only in 2 pups. Virus was detected in the feces for 4.5, 6.5, and 46 median days by hemagglutination, virus isolation on cell cultures, and real-time polymerase chain reaction (PCR), respectively. By real-time PCR, the highest viral DNA titers were detected in the feces of both litters at day 10, reaching median values of more than 1010 DNA copies/mg of feces.

scholarly journals Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

2010 ◽  
Vol 30 (11) ◽  
pp. 918-920 ◽  
Author(s):  
Priscilla F Gerber ◽  
Flávia F Pinto ◽  
Marcos B Heinemann ◽  
Zélia I.P Lobato
Keyword(s):  
Real Time ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Polymerase Chain

The dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars.

scholarly journals Molecular Characteristics of the Serological Weak D Phenotype in Koreans

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 920
Author(s):  
Dajeong Jeong ◽  
Sujin Oh ◽  
Eun Young Song ◽  
Yun Ji Hong ◽  
Kyoung Un Park
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Serological Tests ◽  
Molecular Tests ◽  
Specific Amplification ◽  
Polymerase Chain

Serological weak D is a reaction of 2+ or less to anti-D reagent and includes weak D and partial D phenotypes. Although identifying the RhD subtype is important for transfusion safety, serological tests are insufficient for defining the RhD subtype, and molecular tests are needed. To analyze the molecular characteristics of D variants in Koreans to facilitate the formulation of individualized transfusion strategies, molecular tests such as RhD genotyping using real-time polymerase chain reaction (PCR) and partial-D and/or weak-D sequence-specific amplification (SSP) were performed on 105 Korean Rare Blood Program (KRBP) patients exhibiting serological weak D. In total, 58 out of 68 serologically determined weak D KRBP patients were typed as having weak D or partial D phenotypes via RhD genotyping. In detail, eight (13.8%) were typed as partial DVa or DBS, nine (15.5%) as weak D type 15, and four others (6.8%) as partial DVI, partial DVII, weak D type 2, or weak D type 41 or 45, whereas the rest (n = 37, 63.8%) was typed as having either weak D or partial D. This suggests that serological weak D Koreans who require transfusion should be treated as D-negative.

scholarly journals Comparison of Three Rapid Commercial Canine Parvovirus Antigen Detection Tests with Electron Microscopy and Polymerase Chain Reaction

2009 ◽  
Vol 21 (3) ◽  
pp. 344-345 ◽  
Author(s):  
Silke Schmitz ◽  
Christina Coenen ◽  
König Matthias ◽  
Thiel Heinz-Jürgen ◽  
Reto Neiger
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Chronic Diarrhea ◽  
Gastrointestinal Disease ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Group A ◽  
Polymerase Chain ◽  
Group B

Different antibody-based tests for rapid detection of Canine parvovirus antigens in feces are commercially available, allowing quick diagnosis in a clinical setting. However, the diagnostic accuracy of these tests compared with standard methods has not been evaluated so far. In the current study, 3 commercial tests were compared with immune-electron microscopy (IEM) and polymerase chain reaction (PCR). Dogs were divided into 3 groups: group A, samples from dogs with acute hemorrhagic diarrhea ( n = 50); group B, dogs with chronic diarrhea ( n = 10); and group C, dogs with no evidence of gastrointestinal disease ( n = 40). Specificity of all 3 commercial tests versus PCR and IEM was good to excellent (92.2–100%). Sensitivity, in contrast, was poor: 15.8–26.3% versus PCR and 50–60% versus IEM. In group A, 10 dogs were positive by IEM and 24 dogs were positive by PCR. Positive PCR results were also obtained from animals in control groups (group B, 1 dog; group C, 5 dogs). No dog in group B or C was positive by IEM. In conclusion, the rapid tests are useful to diagnose canine parvoviral enteritis, but they do not rule out parvovirus infection in an animal with typical clinical signs. In addition, a small percentage of healthy dogs and dogs with chronic diarrhea showed positive PCR results; this may be due to asymptomatic/persistent infection or intestinal passage of virus. The significance of this finding remains unclear.

scholarly journals First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 182-185 ◽  
Author(s):  
Nilo Fernandes Leça Júnior ◽  
Valter dos Anjos Almeida ◽  
Fábio Santos Carvalho ◽  
George Rego Albuquerque ◽  
Fabiana Lessa Silva
Keyword(s):  
Endemic Area ◽  
Natural Infection ◽  
Clinical Signs ◽  
Domestic Dogs ◽  
Chain Reaction ◽  
First Report ◽  
Molecular Tests ◽  
Southern Bahia ◽  
Polymerase Chain ◽  
Cruzi Infection

In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.

scholarly journals Carbapenemase Production and Epidemiological Characteristics of Carbapenem-Resistant Klebsiella pneumoniae in Western Chongqing, China

2022 ◽  
Vol 11 ◽  
Author(s):  
Wan Huang ◽  
Jisheng Zhang ◽  
Lingyi Zeng ◽  
Chengru Yang ◽  
Lining Yin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Klebsiella Pneumoniae ◽  
Virulence Genes ◽  
Resistance Rate ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Detection Rates ◽  
Carbapenem Resistant ◽  
Polymerase Chain ◽  
Epidemiological Characteristics

BackgroundThis study aimed to determine the molecular characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates in a hospital in western Chongqing, southwestern China.MethodsA total of 127 unique CRKP isolates were collected from the Yongchuan Hospital of Chongqing Medical University, identified using a VITEK-2 compact system, and subjected to microbroth dilution to determine the minimal inhibitory concentration. Enterobacteriaceae intergenic repeat consensus polymerase chain reaction and multilocus sequence typing were used to analyze the homology among the isolates. Genetic information, including resistance and virulence genes, was assessed using polymerase chain reaction. The genomic features of the CRKP carrying gene blaKPC-2 were detected using whole-genome sequencing.ResultsST11 was the dominant sequence type in the homology comparison. The resistance rate to ceftazidime-avibactam in children was much higher than that in adults as was the detection rate of the resistance gene blaNDM (p < 0.0001). Virulence genes such as mrkD (97.6%), uge (96.9%), kpn (96.9%), and fim-H (84.3%) had high detection rates. IncF (57.5%) was the major replicon plasmid detected, and sequencing showed that the CRKP063 genome contained two plasmids. The plasmid carrying blaKPC-2, which mediates carbapenem resistance, was located on the 359,625 base pair plasmid IncFII, together with virulence factors, plasmid replication protein (rep B), stabilizing protein (par A), and type IV secretion system (T4SS) proteins that mediate plasmid conjugation transfer.ConclusionOur study aids in understanding the prevalence of CRKP in this hospital and the significant differences between children and adults, thus providing new ideas for clinical empirical use of antibiotics.

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