Method development of ramipril and atorvastatin calcium capsule by using simultaneous equation method and absorbance ratio method

2014 ◽  
Vol 8 (4) ◽  
pp. 271
Author(s):  
JS Rajawat ◽  
P Dadeech ◽  
AD Ankalgi ◽  
MS Ranawat ◽  
MS Panwar
Keyword(s):  
Method Development ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Atorvastatin Calcium ◽  
Absorbance Ratio

scholarly journals Development and Validation for the Simultaneous Quantification of Nebivolol Hydrochloride and Hydrochlorothiazide by UV Spectroscopy, RP-HPLC and HPTLC in Tablets

2010 ◽  
Vol 7 (2) ◽  
pp. 341-348 ◽  
Author(s):  
B. Dhandapani ◽  
N. Thirumoorthy ◽  
D. Jose Prakash
Keyword(s):  
Mobile Phase ◽  
Uv Spectroscopy ◽  
Internal Standard ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Simultaneous Quantification ◽  
Rp Hplc ◽  
Absorbance Ratio ◽  
Nebivolol Hydrochloride

Simultaneous quantification of nebivolol hydrochloride (NEB-H) and hydrochlorothiazide (HCT) in tablets by UV spectroscopy, RP-HPLC and HPTLC methods were developed. In UV spectrophotometric determination NEB-H and HCT was quantified by simultaneous equation method and absorbance ratio method. In simultaneous equation method absorbance measurements at 282.5 nm (λmaxNEB-H) and 271.5 nm (λmaxHCT), in absorbance ratio method absorbance measurements at 282.5 nm and 275 nm (iso absorptive point) in methanol. In RP-HPLC method, the drugs were resolved using a mobile phase of 30 mM phosphate buffer (K2HPO4), acetonitrile and triethylamine (50:50:0.1 % v/v) with pH 5.5 using orthophosphoric acid on a C18-ODS- Phenomenex (5 μm, 250 mm x 4.6 mm) column in isocratic mode, Atorvastatin (ATR) used as a internal standard. The retention time of HCT, NEB-H and ATR was 3.31, 4.30 and 6.93 min respectively. In the HPTLC method, the chromatograms were developed using a mobile phase of ethyl acetate: methanol: ammonia (8.5:1:0.5 v/v) on precoated plate of silica gel 60 F254and quantified by densitometric absorbance mode at 285 nm. The Rf of HCT and NEB-H were 0.21 and 0.41 respectively. Recovery studies of 98.88-102.41%, percentage relative std deviation of not more than 0.8 and correlation coefficient (linearity range) of 0.9954-0.9999 shows that developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing NEB-H and HCT.

scholarly journals ULTRAVIOLET SPECTROSCOPY AND ITS PHARMACEUTICAL APPLICATIONS- A BRIEF REVIEW

2018 ◽  
Vol 11 (2) ◽  
pp. 59 ◽  
Author(s):  
Dipali M Atole ◽  
Hrishikesh H Rajput
Keyword(s):  
Simultaneous Equation ◽  
Ratio Method ◽  
Isosbestic Point ◽  
Complex Matrix ◽  
Spectrophotometric Methods ◽  
Factor Method ◽  
Absorbance Ratio ◽  
Present Information ◽  
Different Types ◽  
Absorbance Difference

 Rapid and easy analytical methods are needed due to increasing number of multicomponent formulations, biotherapeutic products and samples of complex matrix in que. Number of Ultraviolet (UV) spectrophotometric methods used for these purpose. Different types of UV spectrometric methods developed on the basis of principle of additivity, absorbance difference, processing absorption spectra. The aim of this review is to present information on simultaneous equation method, difference spectrophotometry, derivative spectrophotometry, absorbance ratio spectra, derivative ratio spectra, successive ratio - derivative spectra, Q-absorbance ratio method, absorptivity factor method, dual wavelength method, absorption factor method, multivariate chemometric methods, and isosbestic point method. A brief summary on theories, mathematical background and some applications of these methods are presented here.

METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND NATEGLINIDE IN COMBINED DOSAGE FORM BY UV SPECTROSCOPY.

2020 ◽  
Vol 7 (9) ◽  
pp. 16-21
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Quantitative Estimation ◽  
Uv Spectroscopy ◽  
Simultaneous Equation ◽  
Dosage Forms ◽  
Chemotherapeutic Agents ◽  
Simultaneous Estimation ◽  
Atorvastatin Calcium ◽  
Ultraviolet Absorbance

Keywords: Simultaneous estimation, combined dosage forms, Atorvastatin, Nateglinide. In the present work, the quantitative estimation of both the drugs in combined dosage forms was carried out. A new, simple, reliable, sensitive, rapid, and economical procedure for simultaneous estimation of atorvastatin calcium and nateglinide in a combined dosage form by UV spectroscopy using the simultaneous equation method was developed. Native ultraviolet absorbance maxima of the two chemotherapeutic agents were used. As both compounds do not interact chemically in methanol, the two wavelengths 246.15 nm for atorvastatin calcium and 206.6 nm for nateglinide were used. Both the drugs obeyed Beer's law in the concentration range (1-10 µg/ml) that was employed in this method. The method developed was validated to determine its linearity (r2=0.996 for atorvastatin and r2=0.997 for nateglinide), precision, reproducibility, and sensitivity. .

scholarly journals A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Gitu Pandey ◽  
Brahmeshwar Mishra
Keyword(s):  
Method Development ◽  
The Other ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Ratio Analysis ◽  
Absorption Ratio ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

A new UV spectrophotometric absorption ratio method was developed and validated for the simultaneous estimation of lamivudine and isoniazid. The method involved Q-absorption ratio analysis using two wavelengths, with one being the λmax of lamivudine (272 nm, λ2) and the other being the isoabsorptive point of both drugs (246 nm, λ1). Beer’s law was obeyed in the concentration range between 5 and 30 µg/mL for both lamivudine and isoniazid. The results of analysis have been validated statistically and by recovery studies as per ICH guidelines. The accuracy ranged between 99.65 and 101.91% and Sandell’s sensitivity ranged between 0.0229 and 0.0347 µg/cm2. The method was found to be simple, precise, reproducible, rapid, and economical. Hence, it could be used in the analysis of laboratory samples and marketed formulations containing these two drugs in the future.

Method Development and Validation for the Estimation of Etizolam and Propranolol Hydrochloride in bulk and Combined Dosage Forms by Simultaneous and Derivative Spectroscopic Methods

2021 ◽  
pp. 263-269
Author(s):  
Susmeena Tabassum Kapatrala ◽  
Vinod Kumar Kondreddy ◽  
Swapna Kandlapalli ◽  
Tejaswi Male
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Spectroscopic Methods ◽  
Propranolol Hydrochloride ◽  
First Derivative ◽  
Derivative Method ◽  
Method Development And Validation ◽  
Development And Validation

Accurate, simple, sensitive and rapid economic UV spectroscopic methods were developed for the estimation of Etizolam and Propranolol Hydrochloride in bulk and combined dosage form. The present study deals with the UV spectroscopic method development and validation for the Simultaneous Equation method and First Derivative method of Etizolam and Propranolol Hydrochloride in bulk and combined dosage form at determined wavelength of Etizolam and Propranolol Hydrochloride at 244nm and 288nm for Simultaneous Equation method and 234nm and 289nm for First Derivative Method. The linearity range for Etizolam and Propranolol Hydrochloride was 1-5µg/ml and 10-50µg/ml, and exhibit good correlation coefficient of Etizolam and Propranolol Hydrochloride was 0.9877 and 0.9977 for Simultaneous Equation method and 0.9872 and 0.9977 for First Derivative method, respectively and excellent mean recovery (98-102%). The precision was found to be within limit (%RSD <2). Comparatively First Derivative method is more sensitive than Simultaneous Equation method. The methods were validated statistically and parameters like linearity, precision, accuracy, specificity and assay was studied according to ICH guidelines and can be applicable in determination of both drugs in routine quality control analysis of drugs in bulk and combined dosage form.

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monika L. Jadhav ◽  
Manoj V. Girase ◽  
Shripad K. Tidme ◽  
Manish S. Junagade
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Absorbance Ratio ◽  
Standard Additions ◽  
Development And Validation ◽  
Tablet Dosage

Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form. The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, λmax for valsartan and hydrochlorothiazide, respectively. The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm (λmax of hydrochlorothiazide). The methods were found to be linear between the range of 5–30 µg/mL for valsartan and 4–24 μg/mL for hydrochlorothiazide using 0.1 N NaOH as solvent. The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions. The precision (intraday, interday) of methods was found within limits (RSD<2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.

scholarly journals SIMULTANEOUS UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CEFADROXIL AND PROBENECID IN TABLET DOSAGE FORM

2018 ◽  
Vol 1 (3) ◽  
pp. 19-23
Author(s):  
Mayur S. Jain ◽  
Sunil R. Bavaskar ◽  
Shashikant D. Barhate ◽  
Jintendra D. Fegade
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Spectrophotometric Methods ◽  
Absorption Ratio ◽  
Tablet Dosage

Two methods for simultaneous estimation of Cefadroxil and Probenecid in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 233 nm and 247 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 242 nm (Isobestic point) and at 247 nm the maximum absorption of Probenecid . The linearity ranges for Cefadroxil and Probenecid both were 10-60μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 99.43±0.75 and 99.69±0.40 for simultaneous equation method and 99.23±0.34 and 99.56±0.16 for absorption ratio method for Cefadroxil and Probenecid respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF VILANTEROL AND FLUTICASONE FUROATE IN PHARMACEUTICAL FORMULATIONS

2017 ◽  
Vol 10 (4) ◽  
pp. 302
Author(s):  
Siva Kishore Masimukku ◽  
Rambabu Chintal
Keyword(s):  
Method Development ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Fluticasone Furoate ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
Development And Validation

Objective:  To develop a simple, rapid,  precise, accurate, sensitive spectrophotometric methods (A&B) were developed for simultaneous estimation and validation of Vilanterol (VTL) and Fluticasone Furoate (FFE) in pure and tablet dosage forms.Method:   Method A is a simultaneous equation method and method B is a first order derivative spectrophotometric method. Pure drug samples of VTL and FFE were dissolved in a mixture of Methanol and Ethanol in the ratio of 1:1 (v/v) and found to have absorbance maxima at 231nm for VTL and 260nm for FFE respectivelyResults:  The linearity lies between 2.5–10µg/ml for VTL and 10–60µg/ml for FFE in these two methods (A&B).  The correlation coefficient (r2) was found to be 0.999 for both VTL and FFE, the limit of detection and limit of quantification were found to be 0.015µg/ml and 0.05µg/ml for VTL and 0.05µg/ml and 0.2µg/ml for FFE respectively. The results of analysis have been validated statistically by recovery studies as per ICH guidelines.Conclusion: The two methods A&B showed good reproducibility and recovery with % RSD less than 2.  Hence both methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of VTL and FFE in pure and combined dosage form.Keywords: Fluticasone furoate, Vilanterol, Derivative spectrophotometric, Simultaneous equation method, Method development and validation.

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