scholarly journals Drug resistant urinary isolates of Pseudomonas Aeruginosa and Acinetobacter species

2010 ◽  
Vol 2 (3) ◽  
pp. 315
Author(s):  
Jyoti Sharma ◽  
Neelam Gulati ◽  
Jagdish Chander
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistant ◽  
Acinetobacter Species ◽  
Urinary Isolates

scholarly journals Effectiveness of Commonly Used Antibiotics in Combination with Honey Against Bacterial Infection

2020 ◽  
Vol 7 ◽  
pp. 51-61
Author(s):  
Niraj Shrestha ◽  
Shova Shrestha ◽  
Bharat Mani Pokharel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Infection ◽  
Drug Resistant ◽  
Inhibitory Effects ◽  
Acinetobacter Species ◽  
The Difference ◽  
Antibiotic Combination

Objectives: The study was carried out to compare the inhibitory effects between commonly used antibiotics and bee honey samples, so as to correlate the inhibitory effects between bee honey alone and in combination with antibiotics. Methods: This study was carried out between December 2012 to September 2013. A total of one hundred and twenty-two clinical microbiological specimens and five different floral sourced honey samples were collected between December 2012 to September 2013. Twenty-three multi-drug resistant organisms were selected. Then, AST for commonly used antibiotics, honey alone and combination of honey-antibiotics discs was done. The difference in ZOI of antibiotic contrasting with the antibiotics containing honey were statistically analysed to define the synergism. Results: The inhibition due to honey is variable among bacteria types (F=39.17, p<0.05). From means plot, Staphylococcus and Acinetobacter were recognized as highly susceptible bacteria for honey (Χ = 21.1 ± 6.2 mm and Χ = 18.3 ± 3.3 mm respectively) but Acinetobacter species could not show synergism to honey-antibiotic combination. The tested organisms from Enterobacteriaceae family showed effective susceptibility to Chloramphenicol-honey mixture. Imipenem-honey combination and Gentamicin-honey combination showed significant effects against Pseudomonas aeruginosa. Conclusion: Thus, honey can be used in various bacteria-directed infections and found to be effective in various infections. Incorporation of honey in antibiotics like Chloramphenicol, Imipenem, and Gentamicin work better in healing various infection.  

scholarly journals 1597. Ceftolozane-Tazobactam and Meropenem Synergy Testing Against Multi-Drug and Extensively-Drug Resistant Pseudomonas aeruginosa

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S794-S795
Author(s):  
Mary Francine P Chua ◽  
Syeda Sara Nida ◽  
Jerry Lawhorn ◽  
Janak Koirala
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Multidrug Resistant ◽  
Additive Effect ◽  
Teaching Hospitals ◽  
Drug Resistant ◽  
Extensively Drug Resistant ◽  
Synergy Testing ◽  
Concentration Indices

Abstract Background Multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa (PA) have limited therapeutic options for treatment. Ceftolozane/tazobactam is a newer anti-pseudomonal drug effective against resistant PA infections, however resistance against this drug has now also developed and is increasing. In this study, we explored the combination of ceftolozane/tazobactam (CT) and meropenem (MP) as a possible effective regimen against MDR and XDR PA. Methods We obtained 33 non-duplicate isolates of MDR and XDR PA grown from blood, urine and respiratory samples collected from patients admitted between 2015 and 2019 at our two affiliate teaching hospitals. MDR PA was defined as resistance to 3 or more classes of anti-pseudomonal antibiotics, and XDR PA as resistance to all but two or less classes of anti-pseudomonal antibiotics. Antimicrobial preparations of both MP and CT were made according to manufacturer instructions. Susceptibility testing was performed using the checkerboard method in accordance to CLSI guidelines (CLSI M100, 2017). The ATCC 27853 strain of PA used as control. Synergy, additive effect, indifference and antagonism were defined as FIC (fractional inhibitory concentration) indices of ≤0.5, &gt;0.5 to &lt;1, &gt;1 to &lt;4, and &gt;4, respectively. Results Thirteen (39%) of 33 PA isolates were classified as XDR, while 20 (61%) PA isolates were MDR. All isolates were resistant to MP (MIC50 &gt;32 ug/mL), while only 2 (6%) isolates were susceptible to CT (MIC50 64 ug/mL). A synergistic effect was seen in 9 (27.3%) of PA isolates (FIC index range 0.28 to 0.5)— 2 of which were XDR PA, and 7 were MDR PA. An additive effect was seen in 12 (36.4%), with indifference seen in 12 (36.4%) of isolates. In this study, no antagonism was seen when CT and MP were combined. Conclusion When used in combination, CT and MP can exert a synergistic effect against MDR and XDR PA. Additive effect and indifference can also be seen when both antibiotics were used. Moreover, there was no antagonism seen when both antibiotics were combined. This study shows that the use of CT and MP in combination may be an option against XDR and MDR PA infections. Disclosures All Authors: No reported disclosures

scholarly journals Genomic analysis of an extensively drug-resistant Pseudomonas aeruginosa ST312 harbouring IncU plasmid-mediated blaKPC-2 isolated from ascitic fluid

2021 ◽  
Vol 25 ◽  
pp. 151-153
Author(s):  
Daniela Cristina Tartari ◽  
Caetana Paes Zamparette ◽  
Graciele Martini ◽  
Sandra Christakis ◽  
Luiz Henrique Costa ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ascitic Fluid ◽  
Genomic Analysis ◽  
Drug Resistant ◽  
Extensively Drug Resistant

scholarly journals Draft Genome Sequence of a Clinically Isolated Extensively Drug-Resistant Pseudomonas aeruginosa Strain

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Bhavani Manivannan ◽  
Niranjana Mahalingam ◽  
Sudhir Jadhao ◽  
Amrita Mishra ◽  
Pravin Nilawe ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Genome Assembly ◽  
Biofilm Formation ◽  
Draft Genome ◽  
Drug Resistant ◽  
Draft Genome Assembly ◽  
Extensively Drug Resistant ◽  
History Of ◽  
Urinary Tuberculosis

We present the draft genome assembly of an extensively drug-resistant (XDR) Pseudomonas aeruginosa strain isolated from a patient with a history of genito urinary tuberculosis. The draft genome is 7,022,546 bp with a G+C content of 65.48%. It carries 7 phage genomes, genes for quorum sensing, biofilm formation, virulence, and antibiotic resistance.

scholarly journals Development of Multi-Drug Resistant Mutants of Clinical Isolates of Pseudomonas aeruginosa after Exposure to Fluoroquinolone

1996 ◽  
Vol 70 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Miyuki HASEGAWA ◽  
Intetsu KOBAYASHI ◽  
Takeshi SAIKA ◽  
Mitsunobu SHIMAZU ◽  
Minoru NISHIDA
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Resistant Mutants

PcrV antibody protects multi-drug resistant Pseudomonas aeruginosa induced acute lung injury

2014 ◽  
Vol 193 ◽  
pp. 21-28 ◽  
Author(s):  
Qin Wang ◽  
Huayin Li ◽  
Jian Zhou ◽  
Ming Zhong ◽  
Duming Zhu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Drug Resistant

Phenotypic and Molecular Characteristics of Pseudomonas Aeruginosa Isolated from Burn Unit

2021 ◽  
Vol 30 (1) ◽  
pp. 19-28
Author(s):  
Yasser M. Ismail ◽  
Sahar M. Fayed ◽  
Fatma M. Elesawy ◽  
Nora Z Abd El-Halim ◽  
Ola S. El-Shimi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Antimicrobial Susceptibility ◽  
Virulence Gene ◽  
Molecular Characteristics ◽  
Drug Resistant ◽  
Burn Wound ◽  
Burn Wounds ◽  
Related Data ◽  
Genes Encoding

Background: The biggest concern for a burn team is a nosocomial infection in burn patients, which is a significant health issue. Pseudomonas aeruginosa is an extremely troublesome drug-resistant bacterium in the world today. We are now faced with rising P. aeruginosa pan-drug-resistant clones in hospital settings. Objectives: To evaluate the distribution of different virulence factors generated by P. aeruginosa isolated from burn wound infections, together with its antimicrobial susceptibility. Methodology: The isolates reported as P. aeruginosa were further tested for the presence of various phenotypic and genotypic virulence factors including (Biofilm formation, lipase, protease, gelatinase, DNase, bile esculin hydrolysis & hemolysin). Also, genes encoding (nan 1 and Exo A) were investigated by PCR using specific primers. All the isolates were tested for their antimicrobial susceptibility patterns. Results: The study reported that toxins and enzymes were expressed by the tested strains in varying proportions; (92.0%) were producing β-hemolysin, lipase (86%), and protease (86%). The formation of biofilm was observed in 84%. Exo A (70%) was the main virulence gene found in the tested strains. Nan 1 gene was identified in 30% of the samples. 82% of MDRPA isolates were found. There is indeed a relationship between biofilm production and drug resistance, as well as the presence of virulence genes (nan 1 and Exo A) were associated with certain patients and burn wounds characteristics as burn size, burn wound depth, length of hospital stays, and socioeconomic status. Conclusions: Correlation of Pseudomonas aeruginosa virulence profiles with burn wounds and patient-related data can be useful in establishing of an appropriate preventive protocol for hospitalized patients with P. aeruginosa burn serious infections. The targeting of these bacterial virulence arsenals is also a promising approach to developing alternative drugs, which act by attenuating the aggressiveness of the pathogen and reducing its potential to cause vigorous infection.

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