Dental pulp stem cells stimulate neuronal differentiation of PC12 cells

Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics ofCd146+,Cd166+, andCd31−in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation ofNestinwas observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereasTub3was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.

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Neuronal differentiation of dental pulp stem cells from human permanent and deciduous teeth following coculture with rat auditory brainstem slices

The Anatomical Record ◽

10.1002/ar.24368 ◽

2020 ◽

Vol 303 (11) ◽

pp. 2931-2946

Author(s):

Thanasup Gonmanee ◽

Hathaitip Sritanaudomchai ◽

Kutkao Vongsavan ◽

Tassanee Faisaikarm ◽

Anupong Songsaad ◽

...

Keyword(s):

Stem Cells ◽

Neuronal Differentiation ◽

Dental Pulp ◽

Auditory Brainstem ◽

Dental Pulp Stem Cells ◽

Deciduous Teeth ◽

Brainstem Slices

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Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair

Stem Cells International ◽

10.1155/2018/1731289 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15 ◽

Cited By ~ 36

Author(s):

Lihua Luo ◽

Yan He ◽

Xiaoyan Wang ◽

Brian Key ◽

Bae Hoon Lee ◽

...

Keyword(s):

Stem Cells ◽

Nervous System ◽

Neuronal Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Neural Regeneration ◽

Differentiation Potential ◽

Neural Repair ◽

Nervous Systems ◽

Potential Applications

This review summarizes current advances in dental pulp stem cells (DPSCs) and their potential applications in the nervous diseases. Injured adult mammalian nervous system has a limited regenerative capacity due to an insufficient pool of precursor cells in both central and peripheral nervous systems. Nerve growth is also constrained by inhibitory factors (associated with central myelin) and barrier tissues (glial scarring). Stem cells, possessing the capacity of self-renewal and multicellular differentiation, promise new therapeutic strategies for overcoming these impediments to neural regeneration. Dental pulp stem cells (DPSCs) derive from a cranial neural crest lineage, retain a remarkable potential for neuronal differentiation, and additionally express multiple factors that are suitable for neuronal and axonal regeneration. DPSCs can also express immunomodulatory factors that stimulate formation of blood vessels and enhance regeneration and repair of injured nerve. These unique properties together with their ready accessibility make DPSCs an attractive cell source for tissue engineering in injured and diseased nervous systems. In this review, we interrogate the neuronal differentiation potential as well as the neuroprotective, neurotrophic, angiogenic, and immunomodulatory properties of DPSCs and its application in the injured nervous system. Taken together, DPSCs are an ideal stem cell resource for therapeutic approaches to neural repair and regeneration in nerve diseases.

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Basic fibroblast growth factor inhibits mineralization but induces neuronal differentiation by human dental pulp stem cells through a FGFR and PLCγ signaling pathway

Journal of Cellular Biochemistry ◽

10.1002/jcb.23097 ◽

2011 ◽

Vol 112 (7) ◽

pp. 1807-1816 ◽

Cited By ~ 63

Author(s):

Thanaphum Osathanon ◽

Nunthawan Nowwarote ◽

Prasit Pavasant

Keyword(s):

Stem Cells ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Signaling Pathway ◽

Neuronal Differentiation ◽

Basic Fibroblast Growth Factor ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Fibroblast Growth ◽

Human Dental Pulp

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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Journal of Visualized Experiments ◽

10.3791/59282 ◽

2019 ◽

Cited By ~ 1

Author(s):

Stefano Martellucci ◽

Costantino Santacroce ◽

Valeria Manganelli ◽

Francesca Santilli ◽

Luca Piccoli ◽

...

Keyword(s):

Stem Cells ◽

Protein Expression ◽

Neuronal Differentiation ◽

Prion Protein ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells

Current Protocols in Human Genetics ◽

10.1002/cphg.28 ◽

2017 ◽

Vol 92 (1) ◽

Cited By ~ 10

Author(s):

Sarita Goorha ◽

Lawrence T. Reiter

Keyword(s):

Stem Cells ◽

Neuronal Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Comparison of the differentiation of dental pulp stem cells and periodontal ligament stem cells into neuron-like cells and their effects on focal cerebral ischemia

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmaa082 ◽

2020 ◽

Vol 52 (9) ◽

pp. 1016-1029

Author(s):

Tingting Wu ◽

Wanting Xu ◽

Hanlin Chen ◽

Shasha Li ◽

Rengang Dou ◽

...

Keyword(s):

Stem Cells ◽

Ischemic Stroke ◽

Cerebral Ischemia ◽

Neuronal Differentiation ◽

Dental Pulp ◽

Periodontal Ligament ◽

Treated Group ◽

Dental Pulp Stem Cells ◽

Therapeutic Effects ◽

Periodontal Ligament Stem Cells

Abstract Recent studies have reported an increasing incidence of ischemic stroke, particularly in younger age groups. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) are the most common stem cells acquired from the teeth of adults, even elderly people. However, there are no detailed reports on whether DPSCs or PDLSCs are suitable for the treatment of ischemic stroke. In this study, the in vitro differentiation of DPSCs and PDLSCs into neuron-like cells was evaluated. Then, we established a rat model of cerebral ischemia. DPSCs or PDLSCs were administered to animals, and the therapeutic effects of these two types of cells were investigated. The results showed that PDLSCs had a higher differentiation rate than DPSCs. Immunofluorescence studies showed that the expression of the neuronal differentiation marker Thy-1 was higher in PDLSCs than in DPSCs, and other gene markers of neuronal differentiation showed corresponding trends, which were confirmed by western blot analysis. In this process, the Notch and Wnt signaling pathways were inhibited and activated, respectively. Finally, rats with transient occlusion of the right middle cerebral artery were used as a model to assess the therapeutic effect of PDLSCs and DPSCs on ischemia. The results showed that rats in the PDLSC-treated group emitted significantly greater red fluorescence signal than the DPSC-treated group. PDLSC transplantation promoted the recovery of neurological function more effectively than DPSC transplantation. Hence, PDLSCs represent an autogenous source of adult mesenchymal stem cells with desirable biological properties and may be an ideal candidate for clinical applications.

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