scholarly journals Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Shahrul Hisham Zainal Ariffin ◽  
Shabnam Kermani ◽  
Intan Zarina Zainol Abidin ◽  
Rohaya Megat Abdul Wahab ◽  
Zulham Yamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Neuronal Differentiation ◽  
Dental Pulp ◽  
Morphological Changes ◽  
Dental Pulp Stem Cells ◽  
Complete Medium ◽  
Free Medium ◽  
Hematopoietic Stem

Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics ofCd146+,Cd166+, andCd31−in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation ofNestinwas observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereasTub3was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.

Dental pulp stem cells in serum-free medium for regenerative medicine

2019 ◽  
Vol 50 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Dawn E. Coates ◽  
Mohammad Alansary ◽  
Lara Friedlander ◽  
Diogo G. Zanicotti ◽  
Warwick J. Duncan
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Comparative Study of the Biological Characteristics of Serum-Free and Fetal Bovine Serum-Contained Medium Cultured Umbilical Cord-Derived Mesenchymal Stem Cells

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4737-4737 ◽  
Author(s):  
Guanghua Chen ◽  
Ting Yang ◽  
Man Qiao ◽  
Huiwen Liu ◽  
Wu Depei
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
Umbilical Cord ◽  
Complete Medium ◽  
Human Umbilical Cord ◽  
Serum Free Medium ◽  
Free Medium ◽  
Early Passage ◽  
Serum Free

Abstract Abstract 4737 Objective: To compare the difference of biological characteristics between human umbilical cord-derived mesenchymal stem cells (UC-MSC) cultured by serum free medium and fetal bovine serum-contained complete medium and to create a xenogeneic protein-free UC-MSC culture system. Methods: Healthy human umbilical cord segments were digested with collagenase. Umbilical cord-derived mesenchymal stem cells were cultured by serum free MesenCult-XF medium and FBS-based αMEM complete medium. We analysed the morphology, immunophenotype, expansion potential, trilineage differentiation potential, karyotype and immunosuppression of early passage of UC-MSC. Results: The average cell diameters of UC-MSC in suspension cultured by serum free medium and FBS-based medium are 26 (18–39) μm and 35 (20–61) μm, respectively. Cell expansion folds with serum free medium and FBS-based medium were (5.2±0.2) and (3.5±0.1) in the first five passage, respectively. The expansion potential of MSCs was significantly higher with serum free medium compared to FBS-based medium (P<0.05). A panel of markers as CD29, CD44, CD90, CD73, CD105 and HLA-ABC were expressed by human UC-MSC. Hematopoietic lineage markers CD34, CD45 and HLA-DR were not detectable on UC-MSC. The cpm were (4.57±0.14)×104, (2.04±0.16)×104 and(0.42±0.04)×104 when serum free medium cultured MSCs were added to the cultures at ratios MSCs/T cell of 1:100, 1:10 and 1:5. While the cpm were (4.57±0.14)×104, (2.04±0.16)×104 and(0.42±0.04)×104when serum free medium cultured UC-MSCs were added to the cultures. The immunosuppressive potential of serum free medium-cultured UC-MSC was higher than that of serum-contained medium cultured UC-MSC at three different ratios MSC/T cell (P<0.05). Conclusion Compare with serum-contained medium cultured early passage of UC-MSC, the cell diameter of serum free medium cultured MSCs was smaller and the expansion potential was higher. No xenogeneic proteins were presented in UC-MSC preparation when UC-MSC was cultured with serum free medium. Human UC-MSC suppresses T-cell proliferation in a dose-dependent manner. The immunosuppressive potential of UC-MSC was higher when cultured in serum free medium compared with FBS-based medium. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Dental pulp stem cells stimulate neuronal differentiation of PC12 cells

2021 ◽  
Vol 16 (9) ◽  
pp. 1821
Author(s):  
Nessma Sultan ◽  
LailaE Amin ◽  
AhmedR Zaher ◽  
MohammedE Grawish ◽  
BenA Scheven
Keyword(s):  
Stem Cells ◽  
Neuronal Differentiation ◽  
Pc12 Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells

scholarly journals Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Lina M. Escobar ◽  
Zita Bendahan ◽  
Andrea Bayona ◽  
Jaime E. Castellanos ◽  
María-Clara González
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Dental Pulp ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Osteoblastic Differentiation ◽  
Dental Pulp Stem Cells ◽  
Alizarin Red ◽  
Human Dental Pulp

Introduction. The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs). Methods. In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods. Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively. Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR. Results. Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h. Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment. After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes. Conclusions. Vitamins D and E induced osteoblastic differentiation of hDPSCs, as evidenced by the decrease in cell proliferation, morphological changes, and the formation of calcified nodules, increasing the expression of differentiation genes. Concurrent treatment with vitamins D + E induces a synergistic effect in differentiation toward an osteoblastic lineage.

Protein Interacting with Never in Mitosis A-1 Induces Glutamatergic and GABAergic Neuronal Differentiation in Human Dental Pulp Stem Cells

2016 ◽  
Vol 42 (7) ◽  
pp. 1055-1061 ◽  
Author(s):  
Young-Ah Cho ◽  
Duck-Su Kim ◽  
Miyeoun Song ◽  
Won-Jung Bae ◽  
Soojung Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neuronal Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

High-purity Hepatic Lineage Differentiated from Dental Pulp Stem Cells in Serum-free Medium

2012 ◽  
Vol 38 (4) ◽  
pp. 475-480 ◽  
Author(s):  
Nikolay Ishkitiev ◽  
Ken Yaegaki ◽  
Toshio Imai ◽  
Tomoko Tanaka ◽  
Taka Nakahara ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Purity ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Tissue Engineering of Necrotic Dental Pulp of Immature Teeth with Apical Periodontitis in Dogs: Radiographic and Histological Evaluation

2018 ◽  
Vol 42 (5) ◽  
pp. 373-382 ◽  
Author(s):  
Eman A El Ashiry ◽  
Najlaa M Alamoudi ◽  
Mahmoud K El Ashiry ◽  
Hagar A Bastawy ◽  
Douaa A El Derwi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Significant Difference ◽  
Group A ◽  
Group B

Aim: To evaluate tissue engineering technology to regenerate pulp-dentin like tissues in pulp canals of immature necrotic permanent teeth with apical periodontitis in dogs. Study design: The study was performed on 36 teeth in 12 dogs. The experiment was carried out using split mouth design. In each dog 3 teeth were selected for implementing the study procedure. Apical periodontitis was induced in Group A and B teeth. Group (A): immature upper left 2nd permanent incisors that were transplanted with a construct of autologous dental pulp stem cells with growth factors seeded in a chitosn hydrogel scaffold. Group (B): immature upper right 2nd permanent incisor that received only growth factors with scaffold. A third tooth in each dog was selected randomly for isolation of dental pulp stem cells (DPSCs). Both groups were closed with a double coronal seal of white MTA (Mineral trioxide aggregate) and glass ionomer cement. Both groups were monitored radiographically for 4 months and histologically after sacrificing the animals. Results: There was no statistically significant difference in radiographic findings between group (A) and group (B) for healing of radiolucencies, while there was statistically significant difference between group (A) and group (B) regarding radicular thickening, root lengthening and apical closure. Histologically, group (A) teeth showed regeneration of pulp- dentin like tissue while group (B) teeth did not show any tissue regeneration. Conclusion: Dental pulp stem cells and growth factors incorporated in chitosan hydrogel are able to regenerate pulp- dentine like tissue and help in complete root maturation of non-vital immature permanent teeth with apical periodontitis in dogs.

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