Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men

ABSTRACTBackgroundChlamydia trachomatis (CT) infection is often mentioned as a silent disease. Reactive oxygen species (ROS) can also cause Sperm apoptosis and have negative impact on Sperm parameters. The objectives of this study were to elucidate the association between Sperm parameters and ROS caused by CT infections resulting in male Infertility as well as evaluating the role of antibiotic therapy.Materials and methodsA total of 848 infertile males having normal and abnormal Sperm parameters were included. After Semen sampling, the CT IgA were measured by Elisa and confirmed by Nested PCR. ROS was determined by Chemiluminescence. After treatment under the direct supervision of the private urologists. Then, the second Semen samples were taken and subjected to tests on Sperm parameters and ROS levels as assessed again.ResultsThe levels of ROS and morphology were improved following the treatments (P<0.05). Antibiotic therapy due to CT infection, could reduce ROS, improve normal morphology and recover some of Semen parameters.ConclusionsOur findings indicate that CT infection and Sperm parameters were associated with the rate of ROS in infertile men. However, after treatment, ROS value dropped allowing the recovery of certain Sperm parameters. Antibiotic therapy can improve some Semen quality parameters and treat the male Infertility.Reza Azmakan, [email protected], Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran, https://orcid.org/0000-0001-6718-3348.

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Stimulated Reactive Oxygen Species Generation in the Spermatozoa of Infertile Men

The Journal of Urology ◽

10.1016/s0022-5347(17)36000-7 ◽

1993 ◽

Vol 149 (1) ◽

pp. 64-67 ◽

Cited By ~ 46

Author(s):

Donald L. Weese ◽

Michael L. Peaster ◽

Kyle K. Himsl ◽

Gary E. Leach ◽

Pramod M. Lad ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species ◽

Infertile Men

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A Study of Role of Reactive Oxygen Species in Idiopathic Male Infertility & Its Management by Antioxidant Therapy in Uttar Pradesh (U.P.)

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/551 ◽

2021 ◽

Vol 8 (32) ◽

pp. 3023-3027

Author(s):

Namrata Shrivastava ◽

Vaibhav Shrivastava ◽

Manish Pandey

Keyword(s):

Reactive Oxygen Species ◽

Male Infertility ◽

Reactive Oxygen ◽

Antioxidant Therapy ◽

Semen Parameters ◽

Control Group ◽

P Value ◽

Ros Generation ◽

Variable Degree ◽

Oxygen Species

BACKGROUND Infertility is defined as the inability to conceive after at 1 year of regular unprotected intercourse. Male contributes to almost half of infertility cases and in almost 30 % of cases, no definite aetiology is identified, and hence, male infertility is labelled idiopathic in these cases. Oxidative energy production mechanisms are almost always accompanied by reactive oxygen species (ROS), generation whose too much concentrations can lead to extensive protein damage and cytoskeletal modifications and inhibit cellular mechanisms. A number of laboratory techniques have been developed to evaluate oxidative stress by measuring ROS level in the semen. In recent times antioxidant supplements have been proposed as useful agents to increase the scavenging capacity of seminal plasma, controversy still surrounds their actual clinical utility. METHODS 34 male patients were included in this study. Reactive oxygen species detection was done by Flowcytometry using dichloroflurosecindiacetate (DCFH-DA). RESULTS The ROS in the patient group was found to be significantly higher 29.821 (5.6300 than the control group 22.162 (1.6331 having p value < 0.001). The ROS (29.821 ± 5.6300) was found to be significantly reduced after 3 months of antioxidant therapy which got reduced to 19.893 ± 4.2299 respectively. CONCLUSIONS Our study demonstrates that infertile men have significantly higher level of ROS (as measured by flowcytometry) & lower sperm count (oligospermia), decreased progressive & total motility and increased immotile sperms as compared to healthy fertile men. This study further proves that antioxidant therapy based on a combination of carnitine, zinc, coq10, lycopene and vitamin C & E for 3 months is associated with a decrease of ROS as measured by flowcytometry & a variable degree of improvement in above mentioned semen parameters. KEYWORDS Reactive Oxygen Species, Male Infertility

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Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

International Journal of Molecular Sciences ◽

10.3390/ijms21165899 ◽

2020 ◽

Vol 21 (16) ◽

pp. 5899 ◽

Cited By ~ 1

Author(s):

Adam Augustyniak ◽

Izabela Pawłowicz ◽

Katarzyna Lechowicz ◽

Karolina Izbiańska-Jankowska ◽

Magdalena Arasimowicz-Jelonek ◽

...

Keyword(s):

Gene Expression ◽

Reactive Oxygen Species ◽

Cold Acclimation ◽

Antioxidant System ◽

Freezing Tolerance ◽

Photosynthetic Apparatus ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Protein Accumulation ◽

Oxygen Species

Though winter-hardiness is a complex trait, freezing tolerance was proved to be its main component. Species from temperate regions acquire tolerance to freezing in a process of cold acclimation, which is associated with the exposure of plants to low but non-freezing temperatures. However, mechanisms of cold acclimation in Lolium-Festuca grasses, important for forage production in Europe, have not been fully recognized. Thus, two L. multiflorum/F. arundinacea introgression forms with distinct freezing tolerance were used herein as models in the comprehensive research to dissect these mechanisms in that group of plants. The work was focused on: (i) analysis of cellular membranes’ integrity; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gas exchange; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); (iii) analysis of plant antioxidant capacity (reactive oxygen species generation; gene expression, protein accumulation, and activity of selected enzymes); and (iv) analysis of Cor14b accumulation, under cold acclimation. The more freezing tolerant introgression form revealed a higher integrity of membranes, an ability to cold acclimate its photosynthetic apparatus and higher water use efficiency after three weeks of cold acclimation, as well as a higher capacity of the antioxidant system and a lower content of reactive oxygen species in low temperature.

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Low amounts of mitochondrial reactive oxygen species define human sperm quality

Reproduction ◽

10.1530/rep-13-0644 ◽

2014 ◽

Vol 147 (6) ◽

pp. 817-824 ◽

Cited By ~ 10

Author(s):

Mónica Marques ◽

Ana Paula Sousa ◽

Artur Paiva ◽

Teresa Almeida-Santos ◽

João Ramalho-Santos

Keyword(s):

Reactive Oxygen Species ◽

Assisted Reproduction ◽

Density Gradient Centrifugation ◽

Sperm Quality ◽

Gradient Centrifugation ◽

Reactive Oxygen ◽

Human Sperm ◽

Semen Parameters ◽

Oxygen Species ◽

Male Gametes

We have applied the mitochondria-specific superoxide fluorescent probe MitoSOX Red (MitoSOX) to detect mitochondria-specific reactive oxygen species (mROS) production in human sperm samples using flow cytometry. We show that human ejaculates are heterogeneous in terms of mROS production, with three subpopulations clearly detectable, comprising sperm that produce increasing amounts of mROS (MitoSOX−, MitoSOX+, and MitoSOX++). The sperm subpopulation producing the lowest amount of mROS represented the most functional subset of male gametes within the ejaculate, as it was correlated with the highest amount of live and non-apoptotic sperm and increased both in samples with better semen parameters and in samples processed by both density-gradient centrifugation and swim-up, both known to select for higher quality sperm. Importantly, the MitoSOX− subpopulation was clearly more prevalent in samples that gave rise to pregnancies following assisted reproduction. Our work, therefore, not only describe discreet human sperm heterogeneity at the mROS level but also suggests that mROS may represent a strategy to both evaluate sperm samples and isolate the most functional gametes for assisted reproduction.Free Portuguese abstractA Portuguese translation of this abstract is freely available athttp://www.reproduction-online.org/content/147/6/817/suppl/DC1

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