Comparison of patient sensitivity using dentin bonding agent versus zinc phosphate base beneath the amalgam restorations: An in vivo comparative study

ABSTRACT Introduction The success of bonded restorations depends on effective bonding between restorative materials and tooth structure, and it prevents microleakage. New dentin bonding systems have been introduced which promotes this concept. The aim of this study was to compare the amount of microleakage between ‘single bond’ vs ‘Prompt L-Pop’ during different time periods. Materials and methods In this clinical trial study, 60 nonretentive class five cavities were prepared on buccal surfaces of human premolars which were scheduled to be removed for orthodontic purposes. The cavities were restored using two different bonding agents; fifth generation (Single Bond) and sixth generation (Prompt L-Pop) and then filled with resin composite (Filtek Z-250). The samples in both groups were extracted after placing the restoration in three periods of time: Immediately, 1 week and 6 months postplacement. Specimens were sealed with two layers of nail varnish except for 1 mm around restoration and immersed in a silver nitrate solution for 24 hours. The teeth were then sectioned longitudinally and the degree of microleakage was determined by stereomicroscope based on standard usual criteria. Results Single bond showed more leakage at dentin margin than enamel margin in all three extraction periods and differences were significant among three groups (p < 0.05). In samples which were restored with Prompt L-Pop only in groups of 6 months there were significant differences between enamel and dentin margins. At enamel margin there were no significant differences between microleakage of groups according to the passage of time (p > 0.05). Conclusion The results of this study using two different bonding systems, indicate that adhesion to enamel was more valuable than dentin and perhaps systems that include self-etching primers are less sensitive to remaining moisture on dentin and they are less affected during different time periods. How to cite this article Samimi P, Barekatain M, Alaei S. Comparison of Microleakage of Composite Restorations using Fifth and Sixth Generation Dentin Bonding Agent: An in vivo Study. J Contemp Dent Pract 2012;13(5):632-636.

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Use of a dentin bonding agent for the fixation of strain gauges on bone

Journal of the Mechanical Behavior of Biomedical Materials ◽

10.1016/j.jmbbm.2021.104545 ◽

2021 ◽

pp. 104545

Author(s):

Tanja Grobecker-Karl ◽

Kamran Orujov ◽

Virgilia Klär ◽

Matthias Karl

Keyword(s):

Bonding Agent ◽

Strain Gauges ◽

Dentin Bonding ◽

Dentin Bonding Agent

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In vitro antibacterial effect of fifth generation dentin bonding agent incorporated with nisin on Streptococcus mutans

Applied Adhesion Science ◽

10.1186/s40563-021-00135-w ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Gopal Keerthipriya ◽

Nesamani Ravikumar ◽

Sekar Mahalaxmi

Keyword(s):

Streptococcus Mutans ◽

Optical Density ◽

Bacterial Growth ◽

Membrane Integrity ◽

Bonding Agent ◽

Control Group ◽

Dentin Bonding ◽

Fifth Generation ◽

Live Bacteria ◽

Dentin Bonding Agent

Abstract Introduction Bacterial adherence to restorative materials such as composite resin is one of the aetiology of secondary caries. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with nisin, against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the experimental bonding agents were obtained using 250 μl Brain Heart Infusion (BHI) broth and the groups were control (BA with 0% Nisin), bonding agent with 1 wt% (NBA 1) and 5 wt% nisin (NBA 5). To this, 10 μl S. mutans culture was added and incubated at 37 °C. Bacterial growth was estimated by changes in optical density using spectrophotometer every 20 min for 2 h. The results were statistically analysed using one way ANOVA followed by Tukey Post Hoc test. For adherence and membrane integrity test, 10 μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 h. After rinsing, 1 ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under confocal laser scanning microscope (CLSM) for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals with maximum value at 2 h (0.83 ± 0.008), while there was a concentration dependant reduction in bacterial growth with the NBA groups (0.50 ± 0.007). Correspondingly, the NBA groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent nisin in dentin bonding agent may render the resin dentin interface more resistant to bacterial penetration, and adherence of cariogenic bacteria like S. mutans.

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