scholarly journals Characterisation of the Human Oral Microbiome in Patients with Coronary Artery Disease Using Next-generation Sequencing of 16SrRNA Amplicons

2017 ◽  
Vol 35 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Thangam Menon ◽  
Sathya Narayanan Gopalakrishnan ◽  
Rayvathy Balasubramanian ◽  
Stalin Roy Justin
Keyword(s):  
Coronary Artery Disease ◽  
Next Generation Sequencing ◽  
Coronary Artery ◽  
Oral Microbiome ◽  
Next Generation ◽  
Artery Disease ◽  
Generation Sequencing

scholarly journals Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Sheng-Nan Chang ◽  
Jien-Jiun Chen ◽  
Jo-Hsuan Wu ◽  
Yao-Te Chung ◽  
Jin-Wun Chen ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Next Generation Sequencing ◽  
Coronary Artery ◽  
Next Generation ◽  
Altered Expression ◽  
Freeze And Thaw ◽  
Micro Rnas ◽  
Exosomal Mirnas ◽  
Artery Disease ◽  
Generation Sequencing

Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.

scholarly journals Composition of the Oral Microbiome in Patients with Coronary Artery Disease

2020 ◽  
Author(s):  
Thangam Menon ◽  
Supraja Kalyanaraman ◽  
Seethalakshmi Srinivasan
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Bacterial Flora ◽  
Human Microbiome ◽  
Oral Microbiome ◽  
Metagenomic Analysis ◽  
Control Group ◽  
Oral Microbiota ◽  
Normal Human ◽  
Artery Disease

Introduction: Distinct microbial communities reside in the oral cavity and the composition of the oral microbiota has important implications for human health and disease. Identification of bacterial flora of the microbiome is done by metagenomic analysis of 16S ribosomal RNA sequences. Aim: The aim of this study was to characterise the human microbiome in patients with Coronary Artery Disease (CAD) in comparison with the normal human microbiome. Materials and Methods: A pilot study was carried out in tertiary hospital, Chennai. Oral mouthwash samples collected from nine patients with CAD were selected, with one control group. They were studied by metagenomic analysis of V3-V4 region of 16SrRNA gene sequences.. Sequencing of the variable V3 and V4 regions was done using Illumina platform. Results: The six major phyla, Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Spirochaetes, and Fusobacteriacontained 99% of the taxa in all the samples analysed. Conclusion: Diversity of the microbiome in patients with CAD was similar to the normal human microbiome.

scholarly journals Next Generation Sequencing Discoveries of the Nitrate-Responsive Oral Microbiome and Its Effect on Vascular Responses

2019 ◽  
Vol 8 (8) ◽  
pp. 1110 ◽  
Author(s):  
Melissa M. Grant ◽  
Daniel Jönsson
Keyword(s):  
Nitric Oxide ◽  
Next Generation Sequencing ◽  
Human Condition ◽  
Oral Microbiome ◽  
Oral Microbiota ◽  
Next Generation ◽  
Contributing Factors ◽  
Future Directions ◽  
Generation Sequencing

Cardiovascular disease is a worldwide human condition which has multiple underlying contributing factors: one of these is long-term increased blood pressure—hypertension. Nitric oxide (NO) is a small nitrogenous radical species that has a number of physiological functions including vasodilation. It can be produced enzymatically through host nitric oxide synthases and by an alternative nitrate–nitrite–NO pathway from ingested inorganic nitrate. It was discovered that this route relies on the ability of the oral microbiota to reduce nitrate to nitrite and NO. Next generation sequencing has been used over the past two decades to gain deeper insight into the microbes involved, their location and the effect of their removal from the oral cavity. This review article presents this research and comments briefly on future directions.

scholarly journals Case Report: Metagenomics Next-Generation Sequencing Can Help Define the Best Therapeutic Strategy for Brain Abscesses Caused by Oral Pathogens

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhonghui Ma ◽  
Su Yan ◽  
Haoxin Dong ◽  
Huifen Wang ◽  
Yonggang Luo ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Brain Abscess ◽  
Genomic Analysis ◽  
Critical Factor ◽  
Oral Microbiome ◽  
Next Generation ◽  
Gram Staining ◽  
Brain Abscesses ◽  
Generation Sequencing

Brain abscesses are associated with an increased long-term risk of new seizures and increased mortality within several years after infection. Common microorganisms that cause brain abscesses include bacteria, fungi, and mycoplasma. We report a 75-year-old man with a brain abscess caused by Prevotella denticola, an oral pathogen. Based on the clinical condition, we suspected that the patient had a blood-borne brain abscess, and he received antibiotics and systemic supportive treatment. The patient developed shock for the second time after negative Gram-staining results. Metagenomics next-generation sequencing showed one strain from the oral microbiome, confirming our hypothesis, and targeted antibiotic treatment was administered quickly. Thus, we report a case in which genomic analysis was the critical factor in determining the best antimicrobial therapy for administration.

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