Protective effect of Carica papaya leaf extract against mercuric chloride-induced nephrotoxicity in wistar rats

Objective: Carica papaya popularly known as papaya or pawpaw is a fruit with diverse nutritional and medicinal properties. The continuous use of the leaf extract as enema by pregnant women necessitated this study on the effect of aqueous extract of the young leaves on the morphometry of the fetuses of pregnant Wistar rats. Material & Methods: Twenty seven sexually matured Wistar rats consisting of eighteen females and nine males, and weighing 180-200g were used for the study. The female rats were assigned equally into three groups of A, B and C. Pregnancies were obtained after the introduction of the males into the female cages overnight. On day 12 of preg-nancy, group A (control) was given sham treatment of tap water, while groups B and C were treated with 60mg/kg and 120mg/kg respectively, of the aqueous leaf extract of C. papaya for seven days (days 12-18 of pregnancy). On day 20 of gestation, the animals were anaesthetized using chloroform and euthanized, and the uteri dissected out to study the fetuses. Results: Results revealed reduced number of viable fetuses, reduced fetal weight, and reduced crown-rump, head, and tail lengths in the group treated with 60mg/kg aqueous leaf extract of C. papaya, while there was resorption of all the fetuses in the group treated with 120mg/kg of the aqueous leaf extract of C. papaya. Conclusion: In conclusion, the aqueous leaf extract of C. papaya at the given dosage in this study is detrimental to developing fetuses, and these effects were dose dependent. Hence, the use of the extract especially during the period of gestation should be discouraged. Key Words: C. papaya; fetus; morphometry; malformation; rats DOI: 10.3126/ajms.v2i1.4028 Asian Journal of Medical Sciences 2 (2011) 18-22

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PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus L.) YANG HIPERGLIKEMIK

JURNAL ILMIAH SAINS ◽

10.35799/jis.17.2.2017.17681 ◽

2017 ◽

Vol 17 (2) ◽

pp. 143

Author(s):

Frendy Grenaldy Tangkumahat ◽

Johnly A Rorong ◽

Feti Ftimah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Carica Papaya ◽

Control Group ◽

Glucose Levels ◽

Flower Extract

PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus L.) YANG HIPERGLIKEMIKABSTRAKTelah dilakukan penelitian tentang Pengaruh Pemberian Ekstrak Bunga dan Daun Pepaya (Carica papaya L.) terhadap Kadar Glukosa Darah Tikus Wistar (Rattus norvegicus L.) yang hiperglikemik. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bunga dan daun pepaya terhadap kadar glukosa darah tikus wistar. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan 30 ekor tikus wistar jantan yang dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol positif (K+) diberi Air, kelompok kontrol negatif (K-) diberi Sukrosa, Ekstrak bunga pepaya dosis 150 mg/kg BB (B1), Ekstrak bunga pepaya dosis 260 mg/kg BB (B2), Ekstrak daun pepaya dosis 100 mg/kg BB (D1) dan Ekstrak daun pepaya dosis 170 mg/kg BB (D2). Data diperoleh dari pemeriksaan kadar glukosa darah puasa setelah tikus diinduksi aloksan secara intraperitional dan setelah masa adaptasi selama 7 hari dan masa perlakuan selama 3 hari setelah diinduksi sediaan per oral (p.o.). Pemberian ekstrak Ethanol bunga dan daun pepaya (Carica Papaya L.) berpengaruh terhadap penurunan kadar glukosa darah tikus wistar (Ratus Norvegicus L.) yang Hiperglikemik. Dosis ekstrak bunga dan daun pepaya yang efektif dalam menurunkan kadar glukosa darah tikus yang hiperglikemia akibat diinduksi dengan aloksan adalah 260 mg/Kg BB dan dosis ekstrak daun 170 mg/Kg BB atau setara dengan bunga dan daun pepaya basah 200 g.Kata Kunci : Carica papaya L, Kadar Glukosa Darah, Aloksan, Hiperglikemik. EFFECT OF FLOWER AND PAPAYA LEAF EXTRACT (Carica papaya L.) ON WISTAR RATS BLOOD GLUCOSE LEVEL (Rattus norvegicus L.) WHICH HYPERGLYCEMIC ABSTRACTA Research had been carried out to determine the Effect of Flower and Papaya Leaf Extract (Carica papaya L.) on Wistar Rats Blood Glucose Level (Rattus norvegicus L.) which hyperglycemic. The purpose of this research is to know the effect of flower and papaya leaf extract on wistar rats blood glucose level. This research used glucose tolerance test method. Used 30 male wistar rats divided into 5 groups of treatment that is positive control group (K+) given Water, negative control group (K-) given Sucrose, papaya flower extract dose 150 mg/kg BW (B1), papaya flower extract dose 260 mg/kg BW (B2), papaya leaf extract dose 100 mg/kg BW (D1) and papaya leaf extract dose 170 mg/kg BW (D2). Data were obtained from fasting blood glucose examination after rats induced alloxan intraperitional and after a 7-day adaptation period and a 3-day treatment period after induced oral preparation (p.o.). The giving of Ethanol flower extract and papaya leaf (Carica Papaya L.) had an effect on the decrease of blood glucose level of Wistar rats (Ratus norvegicus L.) which Hyperglycemic. The dose of flower and papaya leaf extract is effective in lowering blood glucose levels of hyperglycemic rats induced by alloxan is dose of papaya extract 260 mg/Kg BW and dose of leaf extract 170 mg/kg BW or equal of 200g wet papaya flowers and leaves.Keywords: Carica Papaya L, Blood Glucose Levels, Alloxan, Hyperglycemic.

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PROTECTIVE EFFECT OF Geophila obvallata (SHUMACH) DIDR LEAF EXTRACT AND ITS FRACTIONS AGAINST CADMIUM-INDUCED NEPHROTOXICITY IN MALE WISTAR RATS

Toxicology Reports ◽

10.1016/j.toxrep.2021.12.008 ◽

2021 ◽

Author(s):

Lucky Osafanme Iserhienrhien ◽

Okolie Ngozi Paulinus

Keyword(s):

Protective Effect ◽

Wistar Rats ◽

Leaf Extract ◽

Male Wistar Rats

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Effects of Ethyl Acetate Leaf Extract of Carica papaya on Hematological Indices in Plasma of 5 - Fluorouracil Administered Male Wistar Rats

SSRN Electronic Journal ◽

10.2139/ssrn.3568427 ◽

2019 ◽

Author(s):

Fikayo N. Adegboyega ◽

O. T. Adedosu ◽

E. O. Ayoola ◽

G. A. Hamzat ◽

T. O. Abodunrin

Keyword(s):

Ethyl Acetate ◽

Wistar Rats ◽

Leaf Extract ◽

Carica Papaya ◽

Hematological Indices ◽

Male Wistar Rats

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Uji efek ekstrak daun papaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.12291 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Cynthia C.C. Senduk ◽

Henoch Awaloei ◽

Edward Nangoy

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Carica Papaya ◽

Control Group ◽

Elevated Blood ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Sugar Levels

Abstract: Indonesia is a tropical country with more than 9,600 kinds of medicinal plants; one of them is papaya. Extract of papaya leaf (Carica papaya) is presumed to have hypoglycemia effect because it contains flavonoid, alkaloid, saponin, and tannin. This study aimed to evaluate the effect of papaya leaf extract on elevated blood glucose levels on Wistar rats induced with alloxan. This was an experimental study with 18 male Wistar rats as subjects, divided into 6 groups (3 rats in each group). Group 1, the negative control group, was given aquadest only. Group 2, the positive control group, was treated with alloxan 120 mg/kg body weight (BW) followed by novomix 0.2 iu/200 g BW. Group 3 and 4 were treated with alloxan 120 mg/kg BW followed by papaya leaf extract dosing 250 mg and 500 mg/kg BW respectively. Group 5 and 6 were treated with papaya leaf extract dosing 250 mg and 500 mg/kg BW without alloxan induction. Blood glucose levels were measured on day 1, day 2, and day 3 every six hours at 0, 6, 12, 18, and 24 hours. The results showed that 250 mg/kg BW and 500 mg/kg BW of papaya leaf extract could reduce the elevated blood glucose on Wistar rats for 12 hours after treatment. Conclusion: The extract of papaya leaves could reduce blood sugar levels in hyperglicemic Wistar rats induced by alloxan. Keywords: papaya leaves (carica papaya L.), blood sugar levels, alloxan. Abstrak: Indonesia merupakan salah satu negara beriklim tropis yang memiliki banyak jenis tumbuhan. Sekitar 9.600 spesies tumbuhan merupakan tumbuhan yang berkhasiat obat, salah satunya ialah pepaya. Ekstrak daun pepaya (Carica papaya L.) diduga mempunyai efek hipoglikemia karena mengandung flavonoid, alkaloid, saponin, dan tannin. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun pepaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan. Jenis penelitian ini eksperimental. Subyek penelitian yang digunakan ialah 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok (setiap kelompok terdiri dari 3 tikus). Kelompok 1 (K1) merupakan kelompok kontrol negatif hanya diberikan aquades; kelompok 2 (K2) merupakan kelompok kontrol positif diberikan aloksan dan novomix 0,2 iu/200 g BB; kelompok 3 (K3) dan kelompok 4 (K4) merupakan kelompok perlakuan diberikan aloksan kemudian ekstrak daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB; kelompok 5 (K5) dan kelompok 6 (K6) merupakan kelompok perlakuan diberikan daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB tanpa induksi aloksan. Data diperoleh dari pemeriksaan kadar gula darah dari semua kelompok tikus Wistar pada hari ke-1, ke-2, dan ke-3 pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pepaya dengan dosis 250 mg dan 500 mg/kg BB tikus berefek menurunkan kadar gula darah tikus wistar selama 12 jam pasca pemberian ekstrak daun pepaya. Simpulan: Ekstrak daun pepaya berpotensi memiliki efek dalam menurunkan kadar gula darah pada tikus Wistar.Kata kunci: daun pepaya (carica papaya L.), kadar gula darah, aloksan

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