scholarly journals Morphometric Malformations in Fetal Rats Following Treatment with Aqueous Leaf Extract of Carica Papaya

2011 ◽  
Vol 2 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Moses B Ekong ◽  
Mandu U Akpan ◽  
Theresa B Ekanem ◽  
Mfon I Akpaso
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Tap Water ◽  
Female Rats ◽  
Preg Nancy ◽  
Young Leaves ◽  
Group A ◽  
Aqueous Leaf Extract ◽  
Continuous Use

Objective: Carica papaya popularly known as papaya or pawpaw is a fruit with diverse nutritional and medicinal properties. The continuous use of the leaf extract as enema by pregnant women necessitated this study on the effect of aqueous extract of the young leaves on the morphometry of the fetuses of pregnant Wistar rats. Material & Methods: Twenty seven sexually matured Wistar rats consisting of eighteen females and nine males, and weighing 180-200g were used for the study. The female rats were assigned equally into three groups of A, B and C. Pregnancies were obtained after the introduction of the males into the female cages overnight. On day 12 of preg-nancy, group A (control) was given sham treatment of tap water, while groups B and C were treated with 60mg/kg and 120mg/kg respectively, of the aqueous leaf extract of C. papaya for seven days (days 12-18 of pregnancy). On day 20 of gestation, the animals were anaesthetized using chloroform and euthanized, and the uteri dissected out to study the fetuses. Results: Results revealed reduced number of viable fetuses, reduced fetal weight, and reduced crown-rump, head, and tail lengths in the group treated with 60mg/kg aqueous leaf extract of C. papaya, while there was resorption of all the fetuses in the group treated with 120mg/kg of the aqueous leaf extract of C. papaya. Conclusion: In conclusion, the aqueous leaf extract of C. papaya at the given dosage in this study is detrimental to developing fetuses, and these effects were dose dependent. Hence, the use of the extract especially during the period of gestation should be discouraged. Key Words: C. papaya; fetus; morphometry; malformation; rats DOI: 10.3126/ajms.v2i1.4028 Asian Journal of Medical Sciences 2 (2011) 18-22

scholarly journals The Immuno-modulatory and Thrombocytopaenic Effects of the Varying Concentrations of the Aqueous Leaf Extract of Moringa Oleifera in Male Albino Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
Ojeka Sunday Ogbu ◽  
Zabbey Victor Zigabelbari
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Control Group ◽  
Distribution Width ◽  
Treatment Groups ◽  
Immature Platelet Fraction ◽  
Group A ◽  
Aqueous Leaf Extract

Introduction: Moringa oleifera and related species are commonly used in folk medicine for various human diseases. Aim: The study was undertaken to establish the thrombocytopenic effect of the aqueous leaf extract of moringa oleifera and to find the utilization of platelet parameters in determining the cause of the thrombocytopenia. Methodology: Fresh leaves of moringa were dried and extracted with water. Thirty (30) male albino Wistar rats, weighing between 150-250 g, which were kept under uniform laboratory conditions, were randomly divided into five (5) groups (A-E), based on their weights. The control group (group A) was orally given 0.5 ml of distilled water while the treatment groups (groups B to E) were orally given 250 mg/kg, 450 mg/kg,650 mg/kg and 850 mg/kg body weight respectively of the extract, once a day, for 56days and then sacrificed. At the end of the administration, blood samples were collected from each rat and examined for platelet indices. The effects of treatment with aqueous leaf extract of moringa oleifera on the platelet parameters were compared with the control group. Result: The rats treated with the extract, showed a decrease in platelet count and platelet crit while there was a significant increase in the platelet distribution width, mean platelet volume and immature platelet fraction, concerning the control. Conclusion: The aqueous leaf extract of moringa oleifera is therefore shown to modulate the immune system and cause thrombocytopaenia, through platelet destruction.

Histomorphological Effects of Aqueous Leaf Extract of Tefairia occidentalis On the Liver of Wistar Rats.

2019 ◽  
Vol 2 (2) ◽  
pp. 14-29
Author(s):  
B.C. Eze ◽  
D.N. Ezejindu ◽  
E.C. Ogbodo ◽  
I.P. Ezeugwunne ◽  
A.K. Amah ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Female Rats ◽  
Hepatic Tissue ◽  
Tissue Architecture ◽  
Portal Triad ◽  
Telfairia Occidentalis ◽  
Group I ◽  
Aqueous Leaf Extract

Aim: Telfairia occidentalis has been reported to possess numerous health benefits. This study assessed the histomorphological effect of aqueous leaf extract of Tefairia occidentalis on the liver architecture of wistar rats. Methodology: Sixteen adult female rats weighing between 140-240g were divided into four groups (I-IV) comprising of four (4) rats each. Group I, the control, was given normal rat feed with water, while group II, III, and IV were administered with 150mg/kg, 300mg/kg and 600mg/kg body weight of aqueous leaf extract of Telfairia occidentalis orally respectively via intubation method for 28 days. Thereafter, the experimental animals were sacrificed and their respective liver harvested for histomorphological examination using haematoxylin and eosin (H and E) standard laboratory method. Results: Results showed that there was a significant decrease in the relative weight of the liver in groups II-IV when compared to group I respectively (p<0.05). Photomicrographic section of the liver showed normal hepatic tissue architecture with central vain (CV), portal triad (PT) and hepatocyte (H) in the control animals while photomicrograph section of the liver administered with 150mg/kg aqueous extract of Telfairia occidentalis showed hepatic tissue with focal aggregate inflammatory cell (FAIC) and normal tissue architecture with portal triad (PT) but photomicrograph of liver administered with 300mg/kg and 600mg/kg aqueous extract of Telfairia occidentalis showed alterations. Conclusion: This study revealed that the aqueous leaf extracts of Telfairia occidentalis may possess hepatoprotective effects although it may enhance some physiological changes such as mild hemolysis, infiltration of some inflammatory cells and intra-hepatic hemorrhage detected in this study. Key Words: liver, histomorphology, Telfairia occidentalis, weight, hepatoprotective effect.

scholarly journals Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Alian Désiré Afagnigni ◽  
Maximilienne Ascension Nyegue ◽  
Chantal Florentine Ndoye Foe ◽  
Youchahou Njankouo Ndam ◽  
Frédéric Nico Njayou ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Female Rats ◽  
Male Rats ◽  
Dependent Manner ◽  
Subacute Toxicity ◽  
Antidiarrheal Activity ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

acute and sub-acute toxicity studies of aqueous leaf extract of launaea taraxacifolia (willd.) in wistar rats

2021 ◽  
Vol 12 (1) ◽  
pp. 95
Author(s):  
Millicent Umaru ◽  
Kabiru Abubakar ◽  
Gabriel Uyaiabasi ◽  
Giaze Tijjani ◽  
Yusuf Alkali ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Toxicity Studies ◽  
Aqueous Leaf Extract

scholarly journals Effect of Carica papaya (Linn) aqueous leaf extract on pharmacokinetic profile of ciprofloxacin in rabbits

2017 ◽  
Vol 16 (1) ◽  
pp. 127 ◽  
Author(s):  
Grace E. Ukpo ◽  
Mbang A. Owolabi ◽  
Ngozi O.A. Imaga ◽  
Oluwafunke O. Oribayo ◽  
Akpobomen J. Ejiroghene
Keyword(s):  
Leaf Extract ◽  
Carica Papaya ◽  
Pharmacokinetic Profile ◽  
Aqueous Leaf Extract

scholarly journals Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Florence Nalimu ◽  
Joseph Oloro ◽  
Emanuel L. Peter ◽  
Patrick Engeu Ogwang
Keyword(s):  
Toxicity Test ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Female Rats ◽  
Male Rats ◽  
Aqueous Extracts ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Leaf Extracts

Abstract Background Several local communities in Central, Western, Eastern, and Northern regions of Uganda have been using the whole leaf extracts of Aloe vera (L.) Burm. f. (Asphodelaceae) in the treatment of various ailments. Also, several commercial companies sell A. vera as soft drinks in Uganda. However, there are inadequate reports on the toxicities of such preparations. This paper reports the acute and sub-acute oral toxicity of aqueous extracts of whole leaf and green rind of A. vera in Wistar rats. Methods Acute oral toxicity test was carried out in female Wistar rats at doses of 175, 550, 1750, and 5000 mg/kg, p.o. The animals were observed for signs of toxicity for 14 days. Similarly, a sub-acute oral toxicity test was performed in both sexes of rats at doses of 200, 400, and 800 mg/kg, p.o. daily for 28 days. All the groups of animals were monitored for behavioral, morphological, biochemical, and physiological changes, including mortality and compared with respective controls. Body weights were measured weekly while the animals’ relative organ weights, hematological, biochemical, gross, and microscopic pathology were examined on day 29. Results There was no mortality or apparent behavioral changes at the doses tested in acute and sub-acute oral toxicity tests. Thus, the Median Lethal Dose (LD50) of green rind and whole leaf aqueous extracts was above 5000 mg/kg. Gross anatomy revealed that the rats’ relative spleen weight in green rind extract at 200 mg/kg significantly decreased compared to the control group. The creatinine levels in female rats that received green rind extract and the chloride ion levels in male rats administered whole leaf extract were significantly elevated. Conversely, Mean Corpuscular Hemoglobin Concentration (MCHC) levels significantly decreased at lower doses of the green rind extract compared to the control. Histopathology of the kidney revealed the renal interstitium’s inflammation at doses of 200 and 800 mg/kg of the whole leaf extract. Conclusion The findings demonstrated that A. vera green rind and whole leaf extracts are non-toxic at relatively high doses when used for a short duration. Prolonged use of the aqueous whole leaf extract might be associated with kidney toxicity.

Sign in / Sign up

Export Citation Format

Share Document