Independent assortment of seed color and hairy leaf genes in Brassica rapa L.

Rahman, M. 2014. Independent assortment of seed color and hairy leaf genes in Brassica rapa L. Can. J. Plant Sci. 94: 615–620. A genetic study of seed color and hairy leaf in Brassica rapa was conducted in progeny originating from the brown-seeded, hairy leaf B. rapa subsp. chinensis line and the Bangladeshi B. rapa var. trilocularis line. A joint segregation of both traits was also examined in the F2 and backcross populations. Seed color segregated into brown, yellow–brown, and yellow, which suggests that digenic control of brown or yellow–brown color was dominant over yellow seed color. Hairy leaves were found to be under monogenic control, and hairy leaf was dominant over non-hairy leaf. The data show that genes controlling seed color and hairy leaf are inherited independently.

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INHERITANCE OF SEED COAT COLOR IN BRASSICA JUNCEA

Canadian Journal of Plant Science ◽

10.4141/cjps79-100 ◽

1979 ◽

Vol 59 (3) ◽

pp. 635-637 ◽

Cited By ~ 27

Author(s):

C. L. VERA ◽

D. L. WOODS ◽

R. K. DOWNEY

Keyword(s):

Brassica Juncea ◽

Seed Coat ◽

Coat Color ◽

Seed Color ◽

Dominant Allele ◽

Seed Coat Color ◽

Yellow Seed ◽

Brown Color

The genetics of seed coat color inheritance in Brassica juncea (L.) Coss. were studied. It was concluded that this character is controlled by two duplicate pairs of genes (R1, R2) for brown color, either of which can produce brown seed color when a single dominant allele is present. Yellow seed results when all alleles at both loci are recessive.

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Mapping and identification of yellow seed coat color genes in Brassica juncea L.

10.21203/rs.3.rs-25889/v1 ◽

2020 ◽

Author(s):

Zhen Huang ◽

Yang Wang ◽

Hong Lu ◽

Xiang Liu ◽

Lu Liu ◽

...

Keyword(s):

Candidate Gene ◽

Brassica Juncea ◽

Seed Coat ◽

Coat Color ◽

Seed Color ◽

Seed Coat Color ◽

Flavonoid Pathway ◽

Yellow Seed ◽

Color Formation ◽

Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

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A Large Insertion in bHLH Transcription Factor BrTT8 Resulting in Yellow Seed Coat in Brassica rapa

PLoS ONE ◽

10.1371/journal.pone.0044145 ◽

2012 ◽

Vol 7 (9) ◽

pp. e44145 ◽

Cited By ~ 53

Author(s):

Xia Li ◽

Li Chen ◽

Meiyan Hong ◽

Yan Zhang ◽

Feng Zu ◽

...

Keyword(s):

Transcription Factor ◽

Brassica Rapa ◽

Seed Coat ◽

Bhlh Transcription Factor ◽

Yellow Seed ◽

Yellow Seed Coat ◽

Large Insertion

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Inheritance of hull pubescence and seed color in annual canarygrass

Canadian Journal of Plant Science ◽

10.4141/p02-062 ◽

2003 ◽

Vol 83 (3) ◽

pp. 471-474 ◽

Cited By ~ 10

Author(s):

M. A. Matus-Cádiz ◽

P. Hucl ◽

A. Vandenberg

Keyword(s):

Key Words ◽

Growth Stage ◽

Seed Color ◽

Breeding Line ◽

Food Crop ◽

Yellow Seed ◽

Segregation Ratios ◽

Recessive Genes ◽

Hybrid Cross ◽

Phalaris Canariensis

The availability of glabrous-hulled annual canarygrass (Phalaris canariensis L.) cultivars with yellow seed color may pave the way for developing this species into a food crop. The objective of this research was to study the inheritance of hull pubescence and seed color in annual canarygrass. A gametocide was applied to plants at Zadoks Growth Stage 42 to induce male sterility. CDC Maria, a glabrous-hulled and brown-seeded cultivar, was crossed with six pubescent-hulled, brownseeded annual canarygrass accessions and with CY193, a pubescent-hulled and yellow-seeded breeding line. In mono-hybrid crosses, segregation ratios of F2 populations were not significantly different from the phenotypic ratios of 3 pubescent-hulled: 1 glabrous-hulled for hull pubescence and 3 brown seeded: 1 yellow seeded for seed color. In the di-hybrid cross, a phenotypic ratio of 9 pubescent-hulled/brown seeded: 3 pubescent-hulled/yellow seeded: 3 glabrous-hulled/brown seeded: 1 glabrous-hulled/yellow seeded was observed. Glabrous-hulled and yellow seeded traits are each controlled by single recessive genes that segregate independently in annual canarygrass. Key words: Phalaris canariensis, canaryseed, inheritance, hull pubescence, seed color

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Deciphering the transcriptional regulatory networks that control size, color, and oil content in Brassica rapa seeds

10.21203/rs.3.rs-18068/v1 ◽

2020 ◽

Author(s):

Yue Niu ◽

Limin Wu ◽

Yanhua Li ◽

Hualei Huang ◽

Mingchao Qian ◽

...

Keyword(s):

Cell Cycle ◽

Transcription Factor ◽

Brassica Rapa ◽

Seed Yield ◽

Oil Content ◽

Target Genes ◽

Regulatory Mechanisms ◽

Great Promise ◽

Seed Color ◽

Brassica Crops

Abstract Background Brassica rapa is an important oilseed and vegetable crop species and is the A subgenome donor of two important oilseed Brassica crops, Brassica napus and Brassica juncea. Although seed size (SZ), seed color (SC), and oil content (OC) substantially affect seed yield and quality, the mechanisms regulating these traits in Brassica crops remain unclear. Results We collected seeds from a pair of B. rapa accessions with significantly different SZ, SC, and OC at seven seed developmental stages (every 7 days from 7 to 49 days after pollination), and identified 24,835 differentially expressed genes (DEGs) from seven pairwise comparisons between accessions at each developmental stage. K-means clustering identified a group of cell cycle-related genes closely connected to variation in SZ of B. rapa. A weighted correlation analysis using the WGCNA package in R revealed two important co-expression modules comprising genes whose expression was positively correlated with SZ increase and negatively correlated with seed yellowness, respectively. Upregulated expression of cell cycle-related genes in one module was important for the G2/M cell cycle transition, and the transcription factor Bra.A05TSO1 seemed to positively stimulate the expression of two CYCB1;2 genes to promote seed development. In the second module, a conserved complex regulated by the transcription factor TT8 appear to determine SC through downregulation of TT8 and its target genes TT3, TT18, and ANR. Further, upregulation of genes involved in triacylglycerol biosynthesis and storage in the seed oil body may increase OC. We further validated the accuracy of the transcriptome data by quantitative real-time PCR of 15 DEGs. Finally, we used our results to construct detailed models to clarify the regulatory mechanisms underlying variations in SZ, SC, and OC in B. rapa. Conclusions This study based on transcriptome comparison provides insight into the regulatory mechanisms underlying the variations of SZ, SC, and OC in plants. The findings hold great promise for improving seed yield, quality and OC through genetic engineering of critical genes in future molecular breeding.

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Genetic Study and QTL Mapping of Seed Glucosinolate Content in Brassica rapa L.

Crop Science ◽

10.2135/cropsci2013.06.0391 ◽

2014 ◽

Vol 54 (2) ◽

pp. 537-543 ◽

Cited By ~ 10

Author(s):

Habibur Rahman ◽

Berisso Kebede ◽

Céline Zimmerli ◽

Rong-Cai Yang

Keyword(s):

Qtl Mapping ◽

Brassica Rapa ◽

Genetic Study ◽

Glucosinolate Content

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Comparison of linseed (Linum usitatissimum L.) genotypes with respect to the content of polyunsaturated fatty acids

Chemical Papers ◽

10.2478/s11696-012-0209-4 ◽

2012 ◽

Vol 66 (10) ◽

Cited By ~ 7

Author(s):

Marie Bjelková ◽

Janka Nôžková ◽

Katarína Fatrcová-Šramková ◽

Eva Tejklová

Keyword(s):

Fatty Acids ◽

Linoleic Acid ◽

Linear Dependence ◽

Linum Usitatissimum ◽

Oil Content ◽

Seed Color ◽

Alpha Linolenic Acid ◽

Yellow Seed ◽

Linum Usitatissimum L ◽

Total Fat

AbstractThe aim of our work was to characterize linseed (Linum usitatissimum L.) genotypes divided into groups with high and low content of alpha-linolenic acid (ALA). Out of 32 linseed genotypes, 68.75 % represented high alpha-linolenic genotypes and 31.25 % were genotypes with low ALA content. Proportional representation of fatty acids was realized according to the norm (Czech Office for Standards, Metrology and Testing, 1994). Oil content was analyzed according to the internal methodology of Agritec Ltd., based on the norm (Czech Office for Standards, Metrology and Testing, 2011). The content of total fat ranged from 36.22 % to 46.35 %, that of ALA from 1.10 % to 65.20 %, and that of linoleic acid (LA) from 11.10 % to 75.00 % in the analyzed seed samples within all groups. The genotypes were divided also according to the seed color and a linear correlation between all three parameters within these groups was observed. Negative linear dependence was confirmed between parameters; ALA and LA content in the groups: high ALA brown seed (p < 0.0001; correlation coefficient (r) = −0.70), and high ALA yellow seed (p < 0.001; r = −0.36). Also, positive linear dependence between the total fat and the LA content in the groups: low ALA brown seed (p < 0.001; r = 0.34); low ALA yellow seed (p < 0.0001; r = 0.62), was found.

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Comparative transcriptome and flavonoids components analysis reveal the structural genes responsible for the yellow seed coat color of Brassica rapa L.

PeerJ ◽

10.7717/peerj.10770 ◽

2021 ◽

Vol 9 ◽

pp. e10770

Author(s):

Yanjing Ren ◽

Ning Zhang ◽

Ru Li ◽

Xiaomin Ma ◽

Lugang Zhang

Keyword(s):

Brassica Rapa ◽

Seed Coat ◽

Biosynthetic Pathway ◽

Coat Color ◽

Seed Coat Color ◽

Rna Seq ◽

Yellow Seed ◽

Color Formation ◽

Flavonoid Biosynthetic Pathway ◽

Yellow Seed Coat

Background Seed coat color is an important horticultural trait in Brassica crops, which is divided into two categories: brown/black and yellow. Seeds with yellow seed coat color have higher oil quality, higher protein content and lower fiber content. Yellow seed coat color is therefore considered a desirable trait in hybrid breeding of Brassica rapa, Brassica juncea and Brassica napus. Methods Comprehensive analysis of the abundance transcripts for seed coat color at three development stages by RNA-sequencing (RNA-seq) and corresponding flavonoids compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out in B. rapa. Results We identified 41,286 unigenes with 4,989 differentially expressed genes between brown seeds (B147) and yellow seeds (B80) at the same development stage. Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 19 unigenes associated with the phenylpropanoid, flavonoid, flavone and flavonol biosynthetic pathways as involved in seed coat color formation. Interestingly, expression levels of early biosynthetic genes (BrCHS, BrCHI, BrF3H, BrF3’H and BrFLS) in the flavonoid biosynthetic pathway were down-regulated while late biosynthetic genes (BrDFR, BrLDOX and BrBAN) were hardly or not expressed in seeds of B80. At the same time, BrTT8 and BrMYB5 were down-regulated in B80. Results of LC-MS also showed that epicatechin was not detected in seeds of B80. We validated the accuracy of our RNA-seq data by RT-qPCR of nine critical genes. Epicatechin was not detected in seeds of B80 by LC-MS/MS. Conclusions The expression levels of flavonoid biosynthetic pathway genes and the relative content of flavonoid biosynthetic pathway metabolites clearly explained yellow seed color formation in B. rapa. This study provides a foundation for further research on the molecular mechanism of seed coat color formation.

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Localization of QTLs for seed color using recombinant inbred lines of Brassica napus in different environments

Genome ◽

10.1139/g07-068 ◽

2007 ◽

Vol 50 (9) ◽

pp. 840-854 ◽

Cited By ~ 50

Author(s):

Fu-You Fu ◽

Lie-Zhao Liu ◽

You-Rong Chai ◽

Li Chen ◽

Tao Yang ◽

...

Keyword(s):

Brassica Napus ◽

Candidate Gene ◽

Recombinant Inbred ◽

Seed Color ◽

Srap Marker ◽

Linkage Groups ◽

Yellow Seed ◽

Seed Trait ◽

Major Qtl ◽

Selection Of

Yellow seed is one of the most important traits of Brassica napus L. Efficient selection of the yellow-seed trait is one of the most important objectives in oilseed rape breeding. Two recombinant inbred line (RIL) populations (RIL-1 and RIL-2) were analyzed for 2 years at 2 locations. Four hundred and twenty SSR, RAPD, and SRAP marker loci covering 1744 cM were mapped in 26 linkage groups of RIL-1, while 265 loci covering 1135 cM were mapped in 20 linkage groups of RIL-2. A total of 19 QTLs were detected in the 2 populations. A major QTL was detected adjacent to the same marker (EM11ME20/200) in both maps in both years. This major QTL could explain 53.71%, 39.34%, 42.42%, 30.18%, 24.86%, and 15.08% of phenotypic variation in 6 combinations (location × year × population). BLASTn analysis of the sequences of the markers flanking the major QTL revealed that the homologous region corresponding to this major QTL was anchored between genes At5g44440 and At5g49640 of Arabidopsis thaliana chromosome 5 (At C5). Based on comparative genomic analysis, the bifunctional gene TT10 is nearest to the homologue of EM11ME20/200 on At C5 and can be considered an important candidate gene for the major QTL identified here. Besides providing an effective strategy for marker-assisted selection of the yellow-seed trait in B. napus, our results also provide important clues for cloning of the candidate gene corresponding to this major QTL.

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