A RAPID AND SIMPLE ASSAY FOR IDENTIFYING LOW GLUCOSINOLATE RAPESEED

1975 ◽  
Vol 55 (1) ◽  
pp. 191-196 ◽  
Author(s):  
D. I. McGREGOR ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Napus ◽  
Reliable Method ◽  
Glucosinolate Content ◽  
Test Paper ◽  
Brassica Napus L ◽  
Specific Test ◽  
Glucose Test ◽  
The Stability ◽  
Hydrolysis Of ◽  
Seed Extracts

A rapid, simple and reliable method for the identification of seed from low glucosinolate cultivars of rapeseed (Brassica napus L. and B. campestris L.) was developed using a semi-quantitative, glucose-specific test paper containing glucose oxidase, peroxidase and the chromogen, o-tolidine. Glucose released from hydrolysis of the glucosinolates by the endogenous myrosinase in aqueous seed extracts was measured after removal of interfering substances with charcoal. Greater sensitivity at the lower end of the glucose test-paper range allowed identification of low glucosinolate seed from mixtures with normal glucosinolate seeds when the glucosinolate content of the mixture was double, or more, the content of the low glucosinolate seed. The stability of the reagents, sensitivity, simplicity and speed of the assay, approximately 5 min, suggest that it is suitable for the identification of low glucosinolate seed in commerce.

NUTRITIONAL VALUE OF LOW GLUCOSINOLATE RAPESEED MEAL FOR SWINE

1975 ◽  
Vol 55 (1) ◽  
pp. 61-70 ◽  
Author(s):  
J. M. BELL
Keyword(s):  
Brassica Napus ◽  
Soybean Meal ◽  
Nutritional Value ◽  
Control Diet ◽  
Rapeseed Meal ◽  
Glucosinolate Content ◽  
Protein Digestion ◽  
Brassica Napus L ◽  
Ad Libitum

Five swine experiments were conducted to evaluate rapeseed meal (RSM) of low glucosinolate content (Brassica napus L. cv. Bronowski). Two experiments involved 0, 25, 50, 75 and 100% substitution of either Bronowski meal or regular (B. campestris) meal for soybean meal or fishmeal used in the control diet. One experiment compared ad libitum-fed and partially restricted pigs. Another experiment involved digestibility studies, and the final one involved methionine and lysine supplementation. As the dietary levels of either Bronowski or regular RSM increased in the ration, protein digestion coefficients decreased from 79 and 80% to 76 and 78%, respectively, and energy coefficients decreased from 82% to 79 and 78%, respectively. The protein and energy digestibility coefficients for Bronowski RSM were estimated to be 68 and 59%; for regular RSM, 65 and 54%. With barley–wheat–RSM diets, pigs responded to 0.1% methionine, but not to lysine (P > 0.05). Pigs fed ad libitum consumed more Bronowski than regular RSM diet and performed as well as pigs fed soybean meal diets.

scholarly journals Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.

Genes ◽  
2015 ◽  
Vol 6 (4) ◽  
pp. 1215-1229 ◽  
Author(s):  
Cun-Min Qu ◽  
Shi-Meng Li ◽  
Xiu-Jian Duan ◽  
Jin-Hua Fan ◽  
Le-Dong Jia ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Candidate Genes ◽  
Glucosinolate Content ◽  
Brassica Napus L

Variation in the glucosinolate content of vegetative tissues of Chinese lines of Brassica napus L

1999 ◽  
Vol 134 (1) ◽  
pp. 131-136 ◽  
Author(s):  
YUNCHANG LI ◽  
GUY KIDDLE ◽  
RICHARD BENNETT ◽  
KEVIN DOUGHTY ◽  
ROGER WALLSGROVE
Keyword(s):  
Brassica Napus ◽  
Glucosinolate Content ◽  
Brassica Napus L ◽  
Vegetative Tissues

scholarly journals Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

2016 ◽  
Vol 44 (2) ◽  
pp. 435-444
Author(s):  
Imen GLAIED GHRAM ◽  
Hatem BELGUITH ◽  
Maha BEN MUSTAPHA ◽  
Issam HIMILA ◽  
Balkiss BOUHAOUALA ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Expression Patterns ◽  
Pcr Amplification ◽  
Gene Families ◽  
Lipase Gene ◽  
Homologous Proteins ◽  
Brassica Napus L ◽  
Calculated Molecular Mass ◽  
Hydrolysis Of

Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of Brassica napus genomic DNA, with oligonucleotide primers derived from the sequence of an Arabidopsis thaliana homologue. The clone included an open reading frame of 1581 bp encoding a polypeptide of 526 amino acids, with a calculated molecular mass of 59.5 kDa. Analysis of the deduced protein sequence, sequence alignment with homologous proteins from related plant species, and a phylogenetic analysis revealed that the BnLIP protein belongs to the ‘classical’ GxSxG-motif lipase family. RT-PCR assays indicated that the BnLIP gene is expressed specifically, but only transiently, during seed germination: the lipase mRNA was not present at detectable levels in ungerminated seeds, was detected only three days after seed imbibition, but its levels decreased rapidly afterwards. No expression was observed in roots, stems or leaves of adult plants. This expression pattern suggests that BnLIP is one of the lipases involved in the hydrolysis of triacylglycerides stored in rapeseed seeds, ultimately providing nutrients and energy to sustain seedling growth until photosynthesis is activated.

EFFECT OF ALBUGO CANDIDA (PERS. EX CHEV.) KUNTZE ON GROWTH SUBSTANCES IN BRASSICA NAPUS L.

1962 ◽  
Vol 40 (1) ◽  
pp. 53-59 ◽  
Author(s):  
B. I. S. Srivastava ◽  
Michael Shaw ◽  
T. C. Vanterpool
Keyword(s):  
Brassica Napus ◽  
Total Activity ◽  
Growth Substance ◽  
Growth Substances ◽  
Fresh Weight ◽  
Good Source ◽  
Brassica Napus L ◽  
Albugo Candida ◽  
Solvent Systems ◽  
Hydrolysis Of

IAA, IAN, accelerator α, and an ether-insoluble growth substance (designated as 'A') were found in alcohol extracts of healthy inflorescences of Brassica napus L. and in smaller amounts in the malformed, parasitized inflorescences produced by plants infected with Albugo Candida (Pers. ex Chev.) Kuntze. The total activity of diseased extracts (≡ 915 μg IAA/kg fresh weight) was only 61% of that of healthy ones. IAN accounted for approximately 86% of the total activity in both extracts. Brassica is thus a good source of IAN. Both IAA and IAN were identified by their chromogenic reactions, ultraviolet spectra, and Rf values in two solvent systems. Alkaline hydrolysis of 'A' produced an ether-soluble, Ehrlich-positive spot with the same Rf value as IAA. The results are discussed briefly.

Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content

1995 ◽  
Vol 90 (2) ◽  
pp. 194-204 ◽  
Author(s):  
M. Uzunova ◽  
W. Ecke ◽  
K. Weissleder ◽  
G. Röbbelen
Keyword(s):  
Brassica Napus ◽  
Linkage Map ◽  
Glucosinolate Content ◽  
Brassica Napus L

Variation in the glucosinolate content of oilseed rape (Brassica napus L.) leaves.

1991 ◽  
Vol 118 (2) ◽  
pp. 469-477 ◽  
Author(s):  
K. J. DOUGHTY ◽  
A. J. R. PORTER ◽  
A. M. MORTON ◽  
G. KIDDLE ◽  
C. H. BOCK ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Glucosinolate Content ◽  
Brassica Napus L
Sign in / Sign up

Export Citation Format

Share Document