EFFECT OF ALBUGO CANDIDA (PERS. EX CHEV.) KUNTZE ON GROWTH SUBSTANCES IN BRASSICA NAPUS L.

1962 ◽  
Vol 40 (1) ◽  
pp. 53-59 ◽  
Author(s):  
B. I. S. Srivastava ◽  
Michael Shaw ◽  
T. C. Vanterpool
Keyword(s):  
Brassica Napus ◽  
Total Activity ◽  
Growth Substance ◽  
Growth Substances ◽  
Fresh Weight ◽  
Good Source ◽  
Brassica Napus L ◽  
Albugo Candida ◽  
Solvent Systems ◽  
Hydrolysis Of

IAA, IAN, accelerator α, and an ether-insoluble growth substance (designated as 'A') were found in alcohol extracts of healthy inflorescences of Brassica napus L. and in smaller amounts in the malformed, parasitized inflorescences produced by plants infected with Albugo Candida (Pers. ex Chev.) Kuntze. The total activity of diseased extracts (≡ 915 μg IAA/kg fresh weight) was only 61% of that of healthy ones. IAN accounted for approximately 86% of the total activity in both extracts. Brassica is thus a good source of IAN. Both IAA and IAN were identified by their chromogenic reactions, ultraviolet spectra, and Rf values in two solvent systems. Alkaline hydrolysis of 'A' produced an ether-soluble, Ehrlich-positive spot with the same Rf value as IAA. The results are discussed briefly.

scholarly journals Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

2016 ◽  
Vol 44 (2) ◽  
pp. 435-444
Author(s):  
Imen GLAIED GHRAM ◽  
Hatem BELGUITH ◽  
Maha BEN MUSTAPHA ◽  
Issam HIMILA ◽  
Balkiss BOUHAOUALA ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Expression Patterns ◽  
Pcr Amplification ◽  
Gene Families ◽  
Lipase Gene ◽  
Homologous Proteins ◽  
Brassica Napus L ◽  
Calculated Molecular Mass ◽  
Hydrolysis Of

Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of Brassica napus genomic DNA, with oligonucleotide primers derived from the sequence of an Arabidopsis thaliana homologue. The clone included an open reading frame of 1581 bp encoding a polypeptide of 526 amino acids, with a calculated molecular mass of 59.5 kDa. Analysis of the deduced protein sequence, sequence alignment with homologous proteins from related plant species, and a phylogenetic analysis revealed that the BnLIP protein belongs to the ‘classical’ GxSxG-motif lipase family. RT-PCR assays indicated that the BnLIP gene is expressed specifically, but only transiently, during seed germination: the lipase mRNA was not present at detectable levels in ungerminated seeds, was detected only three days after seed imbibition, but its levels decreased rapidly afterwards. No expression was observed in roots, stems or leaves of adult plants. This expression pattern suggests that BnLIP is one of the lipases involved in the hydrolysis of triacylglycerides stored in rapeseed seeds, ultimately providing nutrients and energy to sustain seedling growth until photosynthesis is activated.

INHERITANCE OF YIELD AND OTHER QUANTITATIVE CHARACTERS IN BRASSICA NAPUS VAR. NAPOBRASSICA

1982 ◽  
Vol 24 (4) ◽  
pp. 459-465 ◽  
Author(s):  
I. Grant ◽  
Patricia M. Harney ◽  
B. R. Christie
Keyword(s):  
Brassica Napus ◽  
Combining Ability ◽  
Root Length ◽  
Diallel Cross ◽  
Root Diameter ◽  
Fresh Weight ◽  
Brassica Napus L ◽  
Partial Dominance ◽  
Additive Gene ◽  
Positive Heterosis

Highly significant positive heterosis was found for root length, diameter and fresh weight in F1 hybrids derived in a seven-parent diallel cross of commercially available cultivars of Brassica napus L. var. napobrassica Mill. Significant general combining ability and specific combining ability effects and a preponderance of additive gene action were also noted for root length, diameter and fresh weight. The inheritance of these three traits is thought to be conditioned by additivity at the majority of loci and partial dominance at the remaining ones. Heritability estimates ranged from 0% for dry matter to 26% for root diameter.

scholarly journals Enzymatic Hydrolysis of Kaempferol 3‐ O ‐(2‴‐ O ‐sinapoyl‐ β ‐sophoroside), the Key Bitter Compound of Rapeseed ( Brassica napus L.) Protein Isolate

2021 ◽  
Author(s):  
Mareike Siebert ◽  
Ulrich Krings ◽  
Thorben Günther ◽  
Apostolos Fragalas ◽  
Ralf Günter Berger
Keyword(s):  
Brassica Napus ◽  
Enzymatic Hydrolysis ◽  
Protein Isolate ◽  
Brassica Napus L ◽  
L Protein ◽  
Bitter Compound ◽  
Hydrolysis Of

QTL Mapping for Seedling Dry Weight and Fresh Weight under Salt Stress and Candidate Genes Analysis in Brassica napus L.

2017 ◽  
Vol 43 (2) ◽  
pp. 179
Author(s):  
Lin-Tao HOU ◽  
Teng-Yue WANG ◽  
Hong-Ju JIAN ◽  
Jia WANG ◽  
Jia-Na LI ◽  
...  
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Qtl Mapping ◽  
Candidate Genes ◽  
Dry Weight ◽  
Fresh Weight ◽  
Brassica Napus L

OÖSPORE GERMINATION IN ALBUGO CANDIDA

1959 ◽  
Vol 37 (2) ◽  
pp. 169-172 ◽  
Author(s):  
T. C. Vanterpool
Keyword(s):  
Brassica Napus ◽  
Fresh Water ◽  
Brassica Napus L ◽  
Exit Tube ◽  
Albugo Candida

In addition to the well-known sessile vesicular zoosporangial type of oöspore germination in Albugo candida (Lév.) Kuntze, a second type is described. This consists of an exit tube one to two and a half times the length of the diameter of the oöspore and about one quarter of its width, at the end of which the vesicular zoosporangium is produced. Best results were secured by soaking the oöspores for 7 to 10 days in water at 10° to 12 °C and then changing to fresh water. The oöspore material came from hypertrophied inflorescences of rape (Brassica napus L. var. annua Koch).

Genome-wide association of roots, hypocotyls and fresh weight at germination stage under as stress in Brassica napus L.

2019 ◽  
Vol 45 (2) ◽  
pp. 175
Author(s):  
Cun-Min QU ◽  
Guo-Qiang MA ◽  
Mei-Chen ZHU ◽  
Xiao-Hu HUANG ◽  
Le-Dong JIA ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genome Wide Association ◽  
Fresh Weight ◽  
Brassica Napus L ◽  
Genome Wide ◽  
As Stress ◽  
Germination Stage

scholarly journals Inheritance of resistance to Albugo candida in rape (Brassica napus L.)

1983 ◽  
Vol 25 (5) ◽  
pp. 420-424 ◽  
Author(s):  
Z. Fan ◽  
S. R. Rimmer ◽  
B. R. Stefansson
Keyword(s):  
Brassica Napus ◽  
Genetic Study ◽  
Rust Resistance ◽  
Resistant Cultivar ◽  
Inheritance Of Resistance ◽  
Brassica Napus L ◽  
Albugo Candida ◽  
White Rust ◽  
Dominant Genes

Canadian cultivars of Brassica napus are resistant to white rust caused by Albugo candida while many cultivars of this species grown in China are susceptible. Two Chinese lines susceptible to race 7, GCL, and 2282-9, and one Canadian resistant cultivar, 'Regent,' were chosen for a genetic study of resistance to this pathogen. Inheritance of white rust resistance is conditioned by independent dominant genes at three loci; these were designated Ac7-1, Ac7-2, and Ac7-3. The resistance is conferred by dominance at any one of the three loci and plants with recessive alleles at all loci are susceptible. Since different F2 and BC ratios were obtained for populations derived from different individual plants of 'Regent,' this 'Regent' population is not homogeneous for resistance to white rust. All 'Regent' plants appear to be homogeneous for resistance at two loci while, in addition, some may also carry resistance at a third locus.

A RAPID AND SIMPLE ASSAY FOR IDENTIFYING LOW GLUCOSINOLATE RAPESEED

1975 ◽  
Vol 55 (1) ◽  
pp. 191-196 ◽  
Author(s):  
D. I. McGREGOR ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Napus ◽  
Reliable Method ◽  
Glucosinolate Content ◽  
Test Paper ◽  
Brassica Napus L ◽  
Specific Test ◽  
Glucose Test ◽  
The Stability ◽  
Hydrolysis Of ◽  
Seed Extracts

A rapid, simple and reliable method for the identification of seed from low glucosinolate cultivars of rapeseed (Brassica napus L. and B. campestris L.) was developed using a semi-quantitative, glucose-specific test paper containing glucose oxidase, peroxidase and the chromogen, o-tolidine. Glucose released from hydrolysis of the glucosinolates by the endogenous myrosinase in aqueous seed extracts was measured after removal of interfering substances with charcoal. Greater sensitivity at the lower end of the glucose test-paper range allowed identification of low glucosinolate seed from mixtures with normal glucosinolate seeds when the glucosinolate content of the mixture was double, or more, the content of the low glucosinolate seed. The stability of the reagents, sensitivity, simplicity and speed of the assay, approximately 5 min, suggest that it is suitable for the identification of low glucosinolate seed in commerce.

scholarly journals Antioxidant activity in Brassica napus L. plants expressing lox-dependent BAR gene

1970 ◽  
pp. 271-275
Author(s):  
L. O. Sakhno ◽  
K. V. Lystvan
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Brassica Napus ◽  
Soluble Protein ◽  
Total Soluble Protein ◽  
Bar Gene ◽  
Fresh Weight ◽  
Soluble Protein Content ◽  
Brassica Napus L ◽  
Total Soluble Protein Content

Aim. The study of possible unintended biochemical peculiarities of newly obtained herbicide-resistant canola (Brassica napus L.) plants expressing the lox-dependent BAR gene was the aim. Methods. Total soluble protein content, total free radical scavenging activity, and superoxide dismutase activity have been investigated using Bradford’s, DPPH, and nitroblue tetrazolium assays, respectively. Fresh weight of plants grown on media with or without phosphinothricin was also measured. Results. The antioxidant activity of leaf extracts of untransformed plants under in vitro growth condi-tions had no significant differences in comparison with ones of phospinothricin-resistant plants in the third generation. No significant changes in parameters investigated were observed in transgenic plants cultivated on media with herbicide addition compared to ones grown on media without it. Fresh weight and total soluble protein content were similar in transgenic and untransformed canola plants under growth without phosphinothricin. Conclusions. Lox-dependent BAR gene introduction and expression resulted in no significant differences in leaf antioxidant activity in transgenic canola plants comparing to untransformed controls.Keywords: Brassica napus, antioxidant activity, DPPH, glufosinate, superoxide dismutase.

Sign in / Sign up

Export Citation Format

Share Document