Chemical fractionation of soil boron: I. Method development

1996 ◽  
Vol 76 (4) ◽  
pp. 485-491 ◽  
Author(s):  
J. Hou ◽  
L. J. Evans ◽  
G. A. Spiers
Keyword(s):  
Humic Acid ◽  
Sequential Extraction ◽  
Key Words ◽  
Quartz Sand ◽  
Method Development ◽  
Chemical Fractionation ◽  
Soil Fractionation ◽  
Fractionation Scheme

A chemical fractionation scheme for soil boron was investigated by measuring the amounts of B extracted from minerals and synthetic soils amended with B. The scheme fractionated B into five fractions — readily soluble (solution plus non-specifically adsorbed), specifically adsorbed, oxide bound, organically bound and residual. These five fractions were extracted by 0.01 M CaCl2, 0.05 M KH2PO4, 0.2 M acidic NH4-oxalate, 0.02 M HNO3-30% H2O2, and 1:4:5 HNO3-HF-HCl, respectively. The methodology was tested on goethite and clay mica and three synthetic soils containing mixtures of goethite, clay mica, humic acid, calcite, and quartz sand and amended with three levels of B. Specifically adsorbed and oxide bound B were significantly correlated with the content of goethite in the synthetic soils, while residual B was significantly correlated with the content of clay mica. The recovery of extracted B, estimated as the percentage of total fractions over total B content in minerals and synthetic soils, ranged from 85 to 104%, with a mean of 97%. The overall results obtained with the fractionation scheme were deemed satisfactory as to sensitivity and precision for B fractionation. Key words: Boron, soil fractionation, sequential extraction

scholarly journals Molecular characterization of an end-residue of humeomics applied to a soil humic acid

RSC Advances ◽  
2014 ◽  
Vol 4 (45) ◽  
pp. 23658-23665 ◽  
Author(s):  
A. Nebbioso ◽  
A. Piccolo ◽  
M. Lamshöft ◽  
M. Spiteller
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Molecular Characterization ◽  
Natural Organic Matter ◽  
Molecular Composition ◽  
Chemical Fractionation ◽  
Soil Humic Acid

Humeomics encompasses step-wise chemical fractionation and instrumental determination to fully characterize the heterogeneous molecular composition of natural organic matter.

Humic acid sorption onto a quartz sand surface: A kinetic study and insight into fractionation

2008 ◽  
Vol 325 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Aurélien Pitois ◽  
Liam G. Abrahamsen ◽  
Peter I. Ivanov ◽  
Nick D. Bryan
Keyword(s):  
Humic Acid ◽  
Kinetic Study ◽  
Quartz Sand ◽  
Sand Surface ◽  
Insight Into

METHOD DEVELOPMENT AND DETERMINATION OF PROPIONIC ACID IN FOODS IN HUE CITY

2019 ◽  
pp. 13-18
Author(s):  
Viet Khan Nguyen ◽  
Thi Hoai Nguyen
Keyword(s):  
Key Words ◽  
Propionic Acid ◽  
Method Development ◽  
Food Samples ◽  
Hplc Method ◽  
Optimal Process ◽  
Food Preservative ◽  
System Suitability ◽  
Precision And Accuracy

Background: Propionic acid (PRO) is increasingly being used as a food preservative on markets, today. However, excessive use of this chemical will harm the health of consumers. Objectives: (1) To develope an HPLC method for quantification of PRO in foods and (2) To determinate PRO content in some foods in Hue city. Materials and methods: Breads, cakes, noodles in Hue city. After attaining the optimal process, the method was validated and applied to assess the content of PRO in these materials. Results: The method was validated parameters including: system suitability, specificity, linearity, precision and accuracy. Conclusions: The method development canbe aplied to determine PRO in some foods. PRO contents (using as a preservative) in all foods surveyed in Hue city were under the permitted level of PRO in foods according to Ministry of Health (Vietnam). Key words: propionic acid, food samples, HPLC, content, derivatization

scholarly journals Calf Thymus Composition. A Comparison of Differential Centrifugation and Chemical Fractionation Procedures

1958 ◽  
Vol 4 (6) ◽  
pp. 717-725 ◽  
Author(s):  
Eugene L. Hess ◽  
Saima E. Lagg
Keyword(s):  
Nucleic Acid ◽  
Sequential Extraction ◽  
Extraction Procedure ◽  
Electrophoretic Pattern ◽  
Weight Fraction ◽  
Particulate Material ◽  
Chemical Fractionation ◽  
Nucleic Acid Content ◽  
Suspension Medium ◽  
Differential Sedimentation

The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 M NaCl followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophoretic pattern was obtained from subsequent extracts in the sequence. Sucrose and saline dispersates obtained from tissue fragmented with either the Potter-Elvehjem homogenizer or in a Waring blendor were fractionated, using standard differential sedimentation methods. The fractions obtained by means of four different dispersion procedures were compared in terms of yield, chemical analysis, and electrophoretic composition. The quantity of material in thymus having the sedimentation characteristics of liver mitochondrial and microsomal fractions was remarkably small. Both the suspension medium employed and the method used to bring about a disruption of the cells in the tissue affected the yield of "particulate" material. The components present in the later extracts in the sequence, E4 to E7, in the case of sequential extraction study resembled with respect to chemical composition and electrophoretic characteristics, the microsome fraction prepared by differential sedimentation methods. About 76 per cent of the PNA in the tissue appeared to be in the cytoplasm. The remaining 24 per cent PNA was found in the nucleus and accounted for 1.7 per cent of nucleus on a dry weight basis. From 75 to 88 per cent of cytoplasmic PNA was extracted from the tissue and 76 to 94 per cent of the PNA in the extract was found in the final supernatant solutions, depending upon the dispersion methods and suspension medium used in the extraction procedure. The composition of the final supernatant fractions using differential sedimentation methods were comparable in terms of electrophoretic properties, protein concentration, nucleic acid content, and fractionation behavior to saline extracts E1 to E3, of thymus used in earlier studies.

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