scholarly journals Characterization of membrane-bound fatty acid desaturases

2013 ◽  
Vol 32 (04) ◽  
pp. 445-458 ◽  
Author(s):  
Tatiana Klempova ◽  
Daniel Mihalik ◽  
Milan Certik
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturases ◽  
Membrane Bound

scholarly journals Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0123281 ◽  
Author(s):  
Wei Liu ◽  
Wei Li ◽  
Qiuling He ◽  
Muhammad Khan Daud ◽  
Jinhong Chen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Low Temperature ◽  
Expression Profiles ◽  
Fatty Acid Desaturases ◽  
Genes Encoding ◽  
Gossypium Raimondii ◽  
Membrane Bound

scholarly journals Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini

2004 ◽  
Vol 37 (2) ◽  
pp. 156-173 ◽  
Author(s):  
Andrew W. McCartney ◽  
John M. Dyer ◽  
Preetinder K. Dhanoa ◽  
Peter K. Kim ◽  
David W. Andrews ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturases ◽  
Membrane Bound

scholarly journals Identification of amino acid residues that determine the substrate specificity of mammalian membrane-bound front-end fatty acid desaturases

2015 ◽  
Vol 57 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Kenshi Watanabe ◽  
Makoto Ohno ◽  
Masahiro Taguchi ◽  
Seiji Kawamoto ◽  
Kazuhisa Ono ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Substrate Specificity ◽  
Amino Acid Residues ◽  
Fatty Acid Desaturases ◽  
Front End ◽  
Membrane Bound

scholarly journals Identification and characterization of new Δ-17 fatty acid desaturases

2012 ◽  
Vol 97 (5) ◽  
pp. 1973-1985 ◽  
Author(s):  
Zhixiong Xue ◽  
Hongxian He ◽  
Dieter Hollerbach ◽  
Daniel J. Macool ◽  
Narendra S. Yadav ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturases ◽  
Identification And Characterization

Separation, Partial Purification and Characterization of a Fatty Acid Hydroperoxide Cleaving Enzyme from Apple and Tomato Fruits

1982 ◽  
Vol 37 (3-4) ◽  
pp. 165-173 ◽  
Author(s):  
P. Schreier ◽  
G. Lorenz
Keyword(s):  
Fatty Acid ◽  
Inhibitory Effect ◽  
Fatty Acid Hydroperoxide ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Ph Optimum ◽  
Isoelectric Points ◽  
Acid Hydroperoxide ◽  
Membrane Bound

Abstract A membrane-bound enzyme catalysing the cleavage of 13-hydroperoxy-(Z)-9,(E)-11-oc-tadecadienoic acid (13-LHPO) and 13-hydroperoxy-(Z)-9,(E)-11,(Z)-15-octadecadienoic acid (13-LnHPO) to C6-aldehydes was isolated and partially purified from apples and tomatoes. Attempts to employ Ultrogel AcA 34 and AcA 22 in a gel chromatographic purification step were partially frustrated by reaggregation phenomena. However, by using Sepharose CL-4 B an enzyme fraction (MW 200 000 Da) with lipoxygenase and fatty acid hydroperoxide cleaving activity could be separated from a high molecular-weight active eluate. By applying preparative isoelec­ tric focussing to the tomato protein we succeeded in separating the fatty acid cleaving activity from the lipoxygenase, because o f their different isoelectric points of pH 5.8 -6 .1 and pH 5.0, respectively, An 8.4-fold purification of the fatty acid cleaving activity was achieved. A pH-optimum of 5.5 and a Km-value of 2.6 × 10-5 м/1 for the 13-hydroperoxide of linoleic acid were measured. p-Chloromercuribenzoic acid (1 mм) showed significant inhibitory effect on the fatty acid hydroperoxide cleaving enzyme, but no evidence o f inhibition was found with 1 mм H2O2, KCN, DABCO and EDTA or superoxide dismutase (270 U). The maximum amount of fatty acid hydroperoxide decomposition (C6-aldehyde formation) was determined to be 59%.

scholarly journals New Insight into Phaeodactylum tricornutum Fatty Acid Metabolism. Cloning and Functional Characterization of Plastidial and Microsomal Δ12-Fatty Acid Desaturases

2003 ◽  
Vol 131 (4) ◽  
pp. 1648-1660 ◽  
Author(s):  
Frédéric Domergue ◽  
Patricia Spiekermann ◽  
Jens Lerchl ◽  
Christoph Beckmann ◽  
Oliver Kilian ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Phaeodactylum Tricornutum ◽  
Acid Metabolism ◽  
Functional Characterization ◽  
Fatty Acid Desaturases ◽  
Insight Into

scholarly journals A novel Delta12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115.

2006 ◽  
Vol 53 (4) ◽  
pp. 753-759 ◽  
Author(s):  
D Sh Wei ◽  
M Ch Li ◽  
X X Zhang ◽  
H Zhou ◽  
L J Xing
Keyword(s):  
Fatty Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Methylotrophic Yeast ◽  
Pastoris Gs115 ◽  
Desaturase Gene ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Membrane Bound ◽  
Fatty Acid Desaturase Gene

The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Delta12 and Delta15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Delta12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Delta12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Delta15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Delta12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Delta12-fatty acid desaturase gene in methylotrophic yeast.

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