Spectrophotometric Determination of Uric Acid in Urine Based-Enzymatic Method Uricase with 4-Aminodiphenylamine Diazonium Sulfate (Variamine Blue RT Salt)

2013 ◽  
Vol S7 ◽  
Author(s):  
Hairul Hisham Hamzah
Keyword(s):  
Uric Acid ◽  
Spectrophotometric Determination ◽  
Enzymatic Method ◽  
Variamine Blue

scholarly journals Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent

2003 ◽  
Vol 68 (8-9) ◽  
pp. 691-698 ◽  
Author(s):  
Milena Jelikic-Stankov ◽  
Predrag Djurdjevic ◽  
Dejan Stankov
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Limit Of Detection ◽  
Ascorbate Oxidase ◽  
Acid Oxidation ◽  
Uric Acid Concentration ◽  
Enzymatic Method ◽  
Limit Of Quantification ◽  
Relative Standard

In this work a new enzymatic method for the determination of uric acid in human serum has been developed. The method is based on the oxidative coupling reaction between the N-methyl-N-(4-aminophenyl)-3-methoxyaniline (NCP) reagent and the hydrogen ? donor reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (TOOS), in the system involving three enzymes: uricase, peroxidase and ascorbate oxidase. Using this method uric acid could be determined in concentrations up to 1.428 mmol/L, with a relative standard deviation of up to 1.8 %. The effect of the medium pH and the NCP concentration on the linearity of the chromogen absorbance versus the uric acid concentration curve was investigated. The influence of the uricase activity on the maximum rate of uric acid oxidation was also examined. The use of the NCP reagent demonstrated a more precise and more sensitive determination of the uric acid compared to the determination with 4-aminoantipyrine (4-AA) as the coupling regent. The sensitivity of the method determined from the calibration curve was 0.71 absorbance units per mmol/L of uric acid; the limit of detection was LOD = 0.0035 mmol/L and the limit of quantification was LOQ = 0.015 mmol/L of uric acid.

scholarly journals An ultramicro enzymatic method for the fluorimetric determination of uric acid in serum.

1979 ◽  
Vol 27 (9) ◽  
pp. 2245-2248 ◽  
Author(s):  
HIDEAKI NAGAI ◽  
KIYOSHI ZAITSU ◽  
CHIZUKO HAMADA ◽  
YOSUKE OHKURA
Keyword(s):  
Uric Acid ◽  
Enzymatic Method ◽  
Fluorimetric Determination

Relative Importance of Liver and Kidney in Synthesis of Uric Acid in Chickens

1972 ◽  
Vol 50 (10) ◽  
pp. 936-939 ◽  
Author(s):  
S. T. Chou
Keyword(s):  
Uric Acid ◽  
Spectrophotometric Determination ◽  
Acid Concentration ◽  
Broiler Chickens ◽  
Organ Weight ◽  
Relative Importance ◽  
Nitrogenous Compound ◽  
Inosinic Acid ◽  
Liver And Kidney

Broiler chickens of 1, 2, and 7 months of age were used for enzymatic spectrophotometric determination of uric acid and its precursors, xanthine, hypoxanthine, and inosinic acid, in the liver and kidney in an attempt to show the relative importance of the two organs in the synthesis of uric acid, the major nitrogenous compound excreted by birds.Uric acid concentration of the kidney was two to three times as high as the liver, probably attributable in part to preformed uric acid trapped in the urinary passages in the kidney. Significantly higher concentrations of xanthine, hypoxanthine, and inosinic acid were found in the kidney than in the liver. The calculations based on organ weight and the concentrations of uric acid precursors suggest that the kidney synthesizes 96, 60, and 73% as much uric acid as does the liver for the 1-, 2-, and 7-month-old chickens, respectively.

Enzymatic method for the spectrophotometric determination of aspartame

1994 ◽  
Vol 50 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Erhan Dinçkaya ◽  
Müge Çağin ◽  
Azmi Telefoncu
Keyword(s):  
Spectrophotometric Determination ◽  
Enzymatic Method
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