In Vitro Neuro-Protective Studies of Phyto-Compounds from Azadirachta indica, Centella asiatica and Gloriosa superb

2018 ◽  
Vol 08 (08) ◽  
Author(s):  
Preenon Bagchi ◽  
Ajit Kar ◽  
Anuradha M
2011 ◽  
Vol 133 (2) ◽  
pp. 881-887 ◽  
Author(s):  
Yan Pan ◽  
Badrul Amini Abd-Rashid ◽  
Zakiah Ismail ◽  
Rusli Ismail ◽  
Joon Wah Mak ◽  
...  

2010 ◽  
Vol 36 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Cláudia Regina Dias-Arieira ◽  
Lucas da Rocha Ferreira ◽  
Jailson de Oliveira Arieira ◽  
Edenilson Gonçalves Miguel ◽  
Mateus Augusto Donega ◽  
...  

A flor-preta é uma das doenças mais importantes do morangueiro e a busca por alternativas de controle tem sido uma constante, principalmente em áreas de cultivo orgânico. Assim, objetivou-se avaliar a eficiência, in vitro e in vivo, dos óleos de Eucalyptus citriodora e Azadirachta indica no controle de Colletotrichum acutatum em morangueiro. No experimento in vitro determinou-se a inibição do crescimento micelial quando o fungo foi submetido aos extratos nas concentrações de 0; 0,25; 0,5; 1,0 e 1,5%. No campo, avaliou-se o controle da doença com a aplicação dos óleos nas concentrações de 0, 0,5 e 1,0%, pulverizados em intervalos de 7, 15 e 30 dias, em plantas inoculadas com suspensão de 10(6) conídios/mL. As avaliações foram realizadas semanalmente, observando-se a ocorrência e tamanho de lesões no pedúnculo e nos frutos, abortamento floral, produtividade, e ocorrência natural da doença. In vitro todos os tratamentos apresentaram redução significativa do crescimento micelial do fungo quando comparados ao controle. No campo, apenas o óleo de nim apresentou efeito significativo, reduzindo o abortamento floral e a ocorrência de frutos doentes advindos de flores inoculadas. Porém, maior ocorrência natural de doença foi observada quando a dosagem de 1,0% foi aplicada semanalmente.


2014 ◽  
Vol 49 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Emmanuelle Rodrigues Araújo ◽  
Wolfgang Harand ◽  
Iwanne Coelho Lima ◽  
Fernanda Carolina Ribeiro Dias ◽  
Adelmo Adriane Duarte de Santana ◽  
...  

O objetivo deste trabalho foi avaliar o efeito de extratos de capeba (Piper marginatum) e nim (Azadirachta indica) sobre o fungo Colletotrichum scovillei e determinar o componente mais ativo no controle pós-colheita da antracnose em pimentão. A atividade de extratos metanólicos das folhas (P.marginatum e A. indica) e das sementes (A. indica) a 0, 125, 250, 500, 1.000 e 2.000 ppm, na inibição do crescimento micelial de C. scovillei in vitro foram avaliadas. O extrato metanólico de folhas de P.marginatum foi o mais ativo e, consequentemente, foi submetido ao fracionamento biomonitorado ("bioassay-guided fractionation"). Esse processo rendeu 10 frações majoritárias, obtidas por cromatografia líquida de alta performance, das quais a fração à concentração de 1,5 ppm inibiu o desenvolvimento de C. scovillei de forma mais eficiente do que o fungicida mancozeb.


2009 ◽  
Vol 53 (2) ◽  
pp. 360-364 ◽  
Author(s):  
P. Srivastava ◽  
M. Singh ◽  
P. Mathur ◽  
R. Chaturvedi

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).


2015 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Laureen Michelle Houllou ◽  
Robson Antônio de Souza ◽  
Elizabete Cristina Pacheco dos Santos ◽  
José Jackson Pereira da Silva ◽  
Marta Ribeiro Barbosa ◽  
...  

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.


2016 ◽  
Vol 42 (1) ◽  
pp. 41-48
Author(s):  
Trisha Saha ◽  
Shamim Shamsi

Anthracnose and blight were recorded on Houttuynia cordata Thunb. during April 2013 to December 2013. The isolated fungi from the symptomatic plants were identified as Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporoides (Penz.) Sacc. Ethanol leaf extracts of five plants viz.,Azadirachta indica L., Citrus limon L., Datura metel L., Sennaalata L. and Tagetes erecta L.were evaluated against the pathogenic fungi A. alternata and C. gloeosporoides at 5%, 10% and 20% concentrations in vitro. A. indica recorded as good inhibitor against the test fungi followed by C. limon, S. alata, D. metel and T.erecta. In vivo treatment also showed that A.indica is the most effective in controlling diseases at 10% concentration. The plants treated with A. indica were fresh and healthy up to one month of observation.J. Asiat. Soc. Bangladesh, Sci. 42(1): 41-48, June 2016


2021 ◽  
Vol 12 ◽  
Author(s):  
Jia Hui Wong ◽  
Anna M. Barron ◽  
Jafri Malin Abdullah

Natural products remain a crucial source of drug discovery for accessible and affordable solutions for healthy aging. Centella asiatica (L.) Urb. (CA) is an important medicinal plant with a wide range of ethnomedicinal uses. Past in vivo and in vitro studies have shown that the plant extract and its key components, such as asiatic acid, asiaticoside, madecassic acid and madecassoside, exhibit a range of anti-inflammatory, neuroprotective, and cognitive benefits mechanistically linked to mitoprotective and antioxidant properties of the plant. Mitochondrial dysfunction and oxidative stress are key drivers of aging and neurodegenerative disease, including Alzheimer’s disease and Parkinson’s disease. Here we appraise the growing body of evidence that the mitoprotective and antioxidative effects of CA may potentially be harnessed for the treatment of brain aging and neurodegenerative disease.


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