scholarly journals Evaluation of in vitro Antibacterial and Antioxidant Activity of Aqueous Extracts of Olax psittacorum

Author(s):  
R. Majumder ◽  
Moonmun Dhara ◽  
Lopamudra Adhikari ◽  
G. Ghosh ◽  
S. Pattnaik
Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2386 ◽  
Author(s):  
Delgadillo Claudia ◽  
Cuchillo-Hilario Mario ◽  
Navarro Arturo ◽  
Medina-Campos Omar Noel ◽  
Nieto Antonio ◽  
...  

Background: Acacia farnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacity to curb the inflammation process as well as to down-regulate the pro-inflammatory mediators. Methods: Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessment of anti-inflammatory properties was made by the ear edema induced model in CD-1 mice and MPO activity assay. Likewise, histological analysis, IL-1β, IL-6, IL-10, TNF-α, COX measurements plus nitrite and immunohistochemistry analysis were carried out. Results: Methyl gallate, gallic acid, galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays. Conclusion: All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents.


Pharmacologia ◽  
2016 ◽  
Vol 7 (4) ◽  
pp. 193-201 ◽  
Author(s):  
Joao Batista Teixeir Rocha ◽  
Gerlania de Oliveira Leite ◽  
Albys Ferrer Dubois ◽  
Rodrigo Lopes Seeger ◽  
Aline Augusti Boligon ◽  
...  

2017 ◽  
Vol 69 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Renata de F. Mendes ◽  
Nícolas de C. C. Pinto ◽  
Josiane M. da Silva ◽  
Jucélia B. da Silva ◽  
Raquel C. dos S. Hermisdorf ◽  
...  

2008 ◽  
Vol 56 (3) ◽  
pp. 954-963 ◽  
Author(s):  
Elizabeth Joubert ◽  
E. Siân Richards ◽  
J. Debora Van der Merwe ◽  
Dalene De Beer ◽  
Marena Manley ◽  
...  

Author(s):  
Sanjay Kumar ◽  
Mohammad S Javed ◽  
Pawan Kumar ◽  
Rishendra Kumar

Objectives: The aim of our study was to evaluate the phytogenic chemical compounds and assess their antibacterial and antioxidant activity of essential oil of Ajuga parviflora Benth. growing in the Himalayan region. Methods: In the present study, the phytochemical constituents of essential oil were isolated by steam distillation and screened by gas chromatography (GC) and GC–mass spectrometry (GC-MS) analysis from A. parviflora is rich in oxygenated monoterpenoids and sesquiterpenoids. The essential oil was further evaluated for their antibacterial by well-diffusion method and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay at various concentrations. Results: The major chemical constituents were α-cadinol (21.36%), α-muurolol (14.2%), cubebol (12.76%), germacrene D-4-ol (9.6%), germacrene D (4.32%), farnesyl acetate (3.58%), longifolol acetate (2.76%), and β-bourbonene (2.35 %) whereas monoterpenoids were minor constituents. The zone of inhibition (ZOI) shown by essential oil against test bacterial strains at concentration of 500 μg/ml to Gram-negative bacteria Pseudomonas aeruginosa (5.6 mm), Escherichia coli (5 mm), Salmonella typhimurium (8.6 mm), and Proteus vulgaris (10.7 mm) and Gram-positive bacteria Bacillus subtilis (8.3 mm). At the same time, ZOI of standard antibiotic ciprofloxacin against test bacterial strains was P. aeruginosa (9.6 mm), E. coli (14.3mm), S. typhimurium (19.3 mm), P. vulgaris (17.8 mm), and B. subtilis (20.6 mm), respectively. The free radical scavenging property of A. parviflora employed by in vitro assay methods like scavenging activity of DPPH was 81% at 500 μg/ml concentrations, respectively. Conclusion: Our study showed that α-cadinol, α-muurolol, and cubebol as the major components in this oil which was absent in previous findings of A. parviflora and essential oil had potent antibacterial and antioxidant activity, respectively.


2018 ◽  
Vol 14 (30) ◽  
pp. 338
Author(s):  
Koffi Akessé Georges ◽  
Ahoua Angora Remi Constant ◽  
Ekou Lynda ◽  
Ekou Tchirioua ◽  
Kone Mamidou Witabouna

Skin health is a concern for many people. This aim of this study is to evaluate in vitro the antioxidant activity of 10 plants used in cosmetopoeia for the maintenance of skin health. To achieve this objective, 30 extracts were tested using 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6) acid. -sulfonic) (ABTS + •) assays. The extracts studied were rich in total polyphenols, flavonoids, gallic tannins and alkaloids. For the DPPH reduction, 7 extracts including 5 methanolic and 2 aqueous extracts showed a percentage of inhibition similar to that of Vitamin C (95.79 ± 10.53%) used as a control. With ABTS, 11 extracts (5 methanol, 3 aqueous and 3 hexane) gave reduction percentages close to Trolox (95.97 ± 8.51%). These active plants could play a role in the maintaining of skin.


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