scholarly journals CHEMICAL COMPOSITION, IN VITRO ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM LEAVES OF AJUGA PARVIFLORA BENTH

2018 ◽  
Vol 11 (14) ◽  
pp. 57
Author(s):  
Sanjay Kumar ◽  
Mohammad S Javed ◽  
Pawan Kumar ◽  
Rishendra Kumar
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Vitro Assay ◽  
Germacrene D ◽  
Antibacterial And Antioxidant Activity

Objectives: The aim of our study was to evaluate the phytogenic chemical compounds and assess their antibacterial and antioxidant activity of essential oil of Ajuga parviflora Benth. growing in the Himalayan region. Methods: In the present study, the phytochemical constituents of essential oil were isolated by steam distillation and screened by gas chromatography (GC) and GC–mass spectrometry (GC-MS) analysis from A. parviflora is rich in oxygenated monoterpenoids and sesquiterpenoids. The essential oil was further evaluated for their antibacterial by well-diffusion method and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay at various concentrations. Results: The major chemical constituents were α-cadinol (21.36%), α-muurolol (14.2%), cubebol (12.76%), germacrene D-4-ol (9.6%), germacrene D (4.32%), farnesyl acetate (3.58%), longifolol acetate (2.76%), and β-bourbonene (2.35 %) whereas monoterpenoids were minor constituents. The zone of inhibition (ZOI) shown by essential oil against test bacterial strains at concentration of 500 μg/ml to Gram-negative bacteria Pseudomonas aeruginosa (5.6 mm), Escherichia coli (5 mm), Salmonella typhimurium (8.6 mm), and Proteus vulgaris (10.7 mm) and Gram-positive bacteria Bacillus subtilis (8.3 mm). At the same time, ZOI of standard antibiotic ciprofloxacin against test bacterial strains was P. aeruginosa (9.6 mm), E. coli (14.3mm), S. typhimurium (19.3 mm), P. vulgaris (17.8 mm), and B. subtilis (20.6 mm), respectively. The free radical scavenging property of A. parviflora employed by in vitro assay methods like scavenging activity of DPPH was 81% at 500 μg/ml concentrations, respectively. Conclusion: Our study showed that α-cadinol, α-muurolol, and cubebol as the major components in this oil which was absent in previous findings of A. parviflora and essential oil had potent antibacterial and antioxidant activity, respectively.

scholarly journals Essential Oil from Leaves of Lantana canescens and L. lopez-palacii Grown in Colombia

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Andrés F. Peralta-Bohórquez ◽  
Clara Quijano-Célis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Jorge A. Pino
Keyword(s):  
Essential Oil ◽  
Volatile Compounds ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Enterobacter Sakazakii ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Germacrene D ◽  
In Vitro Antibacterial Activity

The chemical composition of the volatile compounds from the leaves of Lantana canescens Kunth (Verbenaceae) and L. lopez-palacii Moldenke grown in Colombia were analyzed by GC and GC/MS. One hundred and thirty-nine volatile compounds were identified in L. canescens, of which the major ones were β-caryophyllene (13.5%), germacrene D (10.3%) and 1-octen-3-ol (8.4%). In the oil obtained from L. lopez-palacii, eighty-three compounds were identified, of which the most prominent were 1-octen-3-ol (24.4%) and β-caryophyllene (15.2%). The in vitro antibacterial activity of the L. lopez-palacii essential oil was studied against three bacterial strains using the disc diffusion method. No antimicrobial activity was found against Escherichia coli, Enterobacter sakazakii and Listeria monocytogenes.

scholarly journals Chemical Composition, Antibacterial and Antioxidant Activity of the Essential Oil of Bupleurum longiradiatum

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Baojun Shi ◽  
Wei Liu ◽  
Shao-peng Wei ◽  
Wen-jun Wu
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Bupleurum Longiradiatum

The essential oil from the roots of Bupleurum longiradiatum, obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial activity and antioxidant activity. Fifty-one compounds were identified, representing 99.3% of the total oil. The major constituents were thymol (7.0%), butylidene phthalide (6.8%), 5-indolol (5.6%), heptanal (5.3%), 4-hydroxy-2-methylacetophenone (5.3%), 4,5-diethyl-octane (5.3%), bormeol (5.1%) and hexanoic acid (5.1%). The oil was tested against 4 bacteria at different concentrations using disc diffusion and 96-well dilution methods. The inhibition zones and minimum inhibitory concentration values for bacterial strains were in the range of 7.0–18.0 mm and 250 −500 μg/mL, respectively. The in vitro antioxidant activity was assessed by DPPH radical scavenging and inhibition of lipid peroxidation methods. The oil showed a potent free radical scavenging activity, as evidenced by the low IC50 value for DPPH radical (566.2μg/mL) and inhibition of lipid peroxidation (induced by FeSO4, H2O2 and CCl4) with IC50 values of 275.2 μg/mL, 296.9 μg/mL and 118.7 μg/mL, respectively.

Antibacterial and Antioxidant Activity and Essential Oil Composition of Grammosciadium scabridum Boiss. from Iran

2005 ◽  
Vol 60 (7-8) ◽  
pp. 534-538 ◽  
Author(s):  
Ali Sonboli ◽  
Peyman Salehi ◽  
Mohammad Reza Kanani ◽  
Samad Nejad Ebrahimi
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Dpph Assay ◽  
Scavenging Capacity ◽  
Main Components ◽  
Antibacterial And Antioxidant Activity

The in vitro antibacterial and antioxidant activity of the essential oil and its two main components of Grammosciadium scabridum Boiss. (Apiaceae) growing wild in Iran, as well as the composition of its essential oil were studied. A total of 19 compounds representing 99.9% of the oil has been identified. γ-Terpinene (73.5%), p-cymene (14.2%) and (E)-β-farnesene (5.3%) were characterized as the main components. The oil showed remarkable activity against three Gram-negative and four Gram-positive test bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.31 to 10.00 mg/ml. The oil and its two main components were also subjected to screening for their possible antioxidant activity by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The free radical scavenging capacity of the oil was determined with an IC50 value of 6.6 mg/ml.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

10.5219/1695 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1112-1119
Author(s):  
Hana Ďúranová ◽  
Veronika Valková ◽  
Lucia Galovičová ◽  
Jana Štefániková ◽  
Miroslava Kačániová
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Vapor Phase ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Wheat Bread ◽  
Citrus Reticulata ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

In-Vitro Antioxidant, Antibacterial and Anticancer properties of Leaf Extract Annona Muricata

2021 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Anticancer Properties

Annona muricata is one of the important herbal plant that are widely used to treat antidiabetic, anti-inflammatory, insecticidal, antimalarial, anticancer, antibacterial and antioxidant activities. In the present study, extraction procedure was carried out in few step processes method. The extract contains high percentage of steroid, alkaloid, flavonoid, phenolic and saponin. The extract was used to study the antioxidant activity; antibacterial activities and anticancer activity by standard methods. The antioxidant activity was studied by using radical scavenging DPPH, FRAP and H2O2 method and disc diffusion method. The results of antibacterial activity of the following bacteria’s such as Pseudomonas, Staphylococcus, Bacillus, E.coli shows the maximum zone of inhibition against Staphylococcus aureus which is around 1.7 cm. The anticancer activity was carried out by MTT assay using Hep-G2 as cell line and results are reported in the paper.

scholarly journals Essential Oil from Origanum vulgare Completely Inhibits the Growth of Multidrug-Resistant Cystic Fibrosis Pathogens

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Giovanna Pesavento ◽  
Valentina Maggini ◽  
Isabel Maida ◽  
Antonella Lo Nostro ◽  
Carmela Calonico ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Essential Oil ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Achromobacter Xylosoxidans ◽  
Bacterial Strains ◽  
Origanum Vulgare ◽  
Disk Diffusion Method ◽  
Wide Range

Essential oils (EOs) are known to inhibit the growth of a wide range of microorganisms. Particularly interesting is the possible use of EOs to treat multidrug-resistant cystic fibrosis (CF) pathogens. We tested the essential oil (EO) from Origanum vulgare for in vitro antimicrobial activity, against three of the major human opportunistic pathogens responsible for respiratory infections in CF patients; these are methicillin-resistant Staphylococcus aureus, Stenotrophomonas maltophilia and Achromobacter xylosoxidans. Antibiotic susceptibility of each strain was previously tested by the standard disk diffusion method. Most strains were resistant to multiple antibiotics and could be defined as multi-drug-resistant (MDR). The antibacterial activity of O. vulgare EO (OEO) against a panel of 59 bacterial strains was evaluated, with MIC and MBC determined at 24, 48 and 72 hours by a microdilution method. The OEO was effective against all tested strains, although to a different extent. The MBC and MIC of OEO for S. aureus strains were either lower or equal to 0.50%, v/v, for A. xylosoxidans strains were lower or equal to 1% and 0.50%, v/v, respectively; and for S. maltophilia strains were lower or equal to 0.25%, v/v. The results from this study suggest that OEO might exert a role as an antimicrobial in the treatment of CF infections.

scholarly journals Chemical Composition and Antioxidant Activities of the Essential Oil from Tornabenea bischoffii (Apiaceae)

2011 ◽  
Vol 6 (8) ◽  
pp. 1934578X1100600
Author(s):  
Risoleta Ortet ◽  
Erik L. Regalado ◽  
Olivier P. Thomas ◽  
Jorge A. Pino ◽  
Miguel D. Fernández
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Assay ◽  
Cape Verde ◽  
Major Constituent ◽  
Vitro Assay

The chemical composition of Tornabenea bischoffii J. A. Schmidt essential oil from Cape Verde was studied by GC and GC/MS. Sixty volatile compounds were identified of which myristicin was the major constituent (33.6%). Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant antioxidant activities were evidenced.

scholarly journals Composition and Antioxidant Properties of the Essential Oil of the Endemic Cape Verdean Satureja forbesii

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Risoleta Ortet ◽  
Erik L. Regalado ◽  
Olivier P. Thomas ◽  
Jorge A. Pino ◽  
Miguel D. Fernández ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Assay ◽  
Cape Verde ◽  
Cape Verdean ◽  
Vitro Assay ◽  
Aerial Parts

The chemical composition of essential oil from the air-dried aerial parts of Satureja forbesii (Benth.) Briq. from Cape Verde was studied by GC and GC/MS. Thirty-nine volatile compounds were identified of which geranial (42.0%) and neral (31.2%) were the major constituents. Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant activities were evidenced.

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